ID   SEN34_EREGS             Reviewed;         292 AA.
AC   Q754T3;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   24-JAN-2024, entry version 111.
DE   RecName: Full=tRNA-splicing endonuclease subunit SEN34;
DE            EC=4.6.1.16;
DE   AltName: Full=tRNA-intron endonuclease SEN34;
GN   Name=SEN34; OrderedLocusNames=AFL012C;
OS   Eremothecium gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL
OS   Y-1056) (Yeast) (Ashbya gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 51; 53; 70-72; 83-84; 98; 100
RP   AND 102.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Constitutes one of the two catalytic subunit of the tRNA-
CC       splicing endonuclease complex, a complex responsible for identification
CC       and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at
CC       the 5'- and 3'-splice sites to release the intron. The products are an
CC       intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and
CC       5'-OH termini. There are no conserved sequences at the splice sites,
CC       but the intron is invariably located at the same site in the gene,
CC       placing the splice sites an invariant distance from the constant
CC       structural features of the tRNA body. It probably carries the active
CC       site for 3'-splice site cleavage (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-
CC         half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with
CC         a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
CC   -!- SUBUNIT: Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54.
CC       Interacts directly with SEN15 (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family.
CC       {ECO:0000305}.
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DR   EMBL; AE016819; AAS53360.2; -; Genomic_DNA.
DR   RefSeq; NP_985536.2; NM_210890.2.
DR   AlphaFoldDB; Q754T3; -.
DR   STRING; 284811.Q754T3; -.
DR   EnsemblFungi; AAS53360; AAS53360; AGOS_AFL012C.
DR   GeneID; 4621773; -.
DR   KEGG; ago:AGOS_AFL012C; -.
DR   eggNOG; KOG4133; Eukaryota.
DR   HOGENOM; CLU_049366_1_0_1; -.
DR   InParanoid; Q754T3; -.
DR   OMA; FIAYPGD; -.
DR   OrthoDB; 35871at2759; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0000214; C:tRNA-intron endonuclease complex; IEA:EnsemblFungi.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0000213; F:tRNA-intron endonuclease activity; IBA:GO_Central.
DR   GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; IBA:GO_Central.
DR   CDD; cd22363; tRNA-intron_lyase_C; 1.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR   InterPro; IPR006677; tRNA_intron_Endonuc_cat-like.
DR   InterPro; IPR006676; tRNA_splic.
DR   InterPro; IPR016690; tRNA_splic_SEN34.
DR   NCBIfam; TIGR00324; endA; 1.
DR   PANTHER; PTHR13070:SF0; TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34; 1.
DR   PANTHER; PTHR13070; TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34-RELATED; 1.
DR   Pfam; PF01974; tRNA_int_endo; 1.
DR   PIRSF; PIRSF017250; tRNA_splic_SEN34; 1.
DR   SUPFAM; SSF53032; tRNA-intron endonuclease catalytic domain-like; 1.
PE   3: Inferred from homology;
KW   Lyase; Reference proteome; tRNA processing.
FT   CHAIN           1..292
FT                   /note="tRNA-splicing endonuclease subunit SEN34"
FT                   /id="PRO_0000109465"
FT   ACT_SITE        202
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        210
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        243
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   292 AA;  32584 MW;  BFFFBD0B40DC4B6A CRC64;
     MPHTKVAISL NSKTGVPLVF DLDDVRRIRG LGVLGTLCGS LPTAAQQNLF LTVPLRLMLE
     DAVWLVLSGH AYFSFDDKLL VQAAGSLTAA EVCRWRQDTE RGLEQQRELR RREQQLKLAA
     LHRTADETDT QRRLLEQSLF LETENCSKMI AAAEPAGLQL AILREMVAQH LDLGNYLIYR
     YLREEQYFLS AGGRFGARYV AYPGDPLRYH SHMAVQPAMD YYNESLDLLH VVGGGRLGTG
     VKKLWVVGGV RYREAEAAAT AEEQIPPPDT LDRLLATSPP VSVFSIEWSG FG
//