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Q754T3

- SEN34_ASHGO

UniProt

Q754T3 - SEN34_ASHGO

Protein

tRNA-splicing endonuclease subunit SEN34

Gene

SEN34

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (09 Jan 2013)
      Previous versions | rss
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    Functioni

    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei202 – 2021By similarity
    Active sitei210 – 2101By similarity
    Active sitei243 – 2431By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: InterPro

    GO - Biological processi

    1. tRNA-type intron splice site recognition and cleavage Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonuclease subunit SEN34 (EC:4.6.1.16)
    Alternative name(s):
    tRNA-intron endonuclease SEN34
    Gene namesi
    Name:SEN34
    Ordered Locus Names:AFL012C
    OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
    Taxonomic identifieri284811 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
    ProteomesiUP000000591: Chromosome VI

    Subcellular locationi

    GO - Cellular componenti

    1. tRNA-intron endonuclease complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 292292tRNA-splicing endonuclease subunit SEN34PRO_0000109465Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi33169.AGOS_AFL012C.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family.Curated

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000175584.
    KOiK15323.
    OrthoDBiEOG7N905X.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR016690. tRNA_splic_SEN34.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017250. tRNA_splic_SEN34. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q754T3-1 [UniParc]FASTAAdd to Basket

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    MPHTKVAISL NSKTGVPLVF DLDDVRRIRG LGVLGTLCGS LPTAAQQNLF    50
    LTVPLRLMLE DAVWLVLSGH AYFSFDDKLL VQAAGSLTAA EVCRWRQDTE 100
    RGLEQQRELR RREQQLKLAA LHRTADETDT QRRLLEQSLF LETENCSKMI 150
    AAAEPAGLQL AILREMVAQH LDLGNYLIYR YLREEQYFLS AGGRFGARYV 200
    AYPGDPLRYH SHMAVQPAMD YYNESLDLLH VVGGGRLGTG VKKLWVVGGV 250
    RYREAEAAAT AEEQIPPPDT LDRLLATSPP VSVFSIEWSG FG 292
    Length:292
    Mass (Da):32,584
    Last modified:January 9, 2013 - v2
    Checksum:iBFFFBD0B40DC4B6A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016819 Genomic DNA. Translation: AAS53360.2.
    RefSeqiNP_985536.2. NM_210890.2.

    Genome annotation databases

    GeneIDi4621773.
    KEGGiago:AGOS_AFL012C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016819 Genomic DNA. Translation: AAS53360.2 .
    RefSeqi NP_985536.2. NM_210890.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 33169.AGOS_AFL012C.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4621773.
    KEGGi ago:AGOS_AFL012C.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000175584.
    KOi K15323.
    OrthoDBi EOG7N905X.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR016690. tRNA_splic_SEN34.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017250. tRNA_splic_SEN34. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
      Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
      Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
    2. "Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
      Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
      G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 51; 53; 70-72; 83-84; 98; 100 AND 102.
      Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

    Entry informationi

    Entry nameiSEN34_ASHGO
    AccessioniPrimary (citable) accession number: Q754T3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: January 9, 2013
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3