Q754T3 (SEN34_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease subunit SEN34
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease SEN34

Gene names

Name:	SEN34
Ordered Locus Names:	AFL012C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium › Ashbya gossypii

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage By similarity.
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron.
Subunit structure	Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15 By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA-type intron splice site recognition and cleavage Inferred from electronic annotation. Source: InterPro
Cellular_component	tRNA-intron endonuclease complex Inferred from electronic annotation. Source: InterPro
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	tRNA-splicing endonuclease subunit SEN34		PRO_0000109465
Sites
	Active site	202	1	By similarity
	Active site	210	1	By similarity
	Active site	243	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q754T3 [UniParc]. Last modified January 9, 2013. Version 2. Checksum: BFFFBD0B40DC4B6A	FASTA	292	32,584
	10 20 30 40 50 60 MPHTKVAISL NSKTGVPLVF DLDDVRRIRG LGVLGTLCGS LPTAAQQNLF LTVPLRLMLE 70 80 90 100 110 120 DAVWLVLSGH AYFSFDDKLL VQAAGSLTAA EVCRWRQDTE RGLEQQRELR RREQQLKLAA 130 140 150 160 170 180 LHRTADETDT QRRLLEQSLF LETENCSKMI AAAEPAGLQL AILREMVAQH LDLGNYLIYR 190 200 210 220 230 240 YLREEQYFLS AGGRFGARYV AYPGDPLRYH SHMAVQPAMD YYNESLDLLH VVGGGRLGTG 250 260 270 280 290 VKKLWVVGGV RYREAEAAAT AEEQIPPPDT LDRLLATSPP VSVFSIEWSG FG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]	Dietrich F.S., Voegeli S., Philippsen P. Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 51; 53; 70-72; 83-84; 98; 100 AND 102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016819 Genomic DNA. Translation: AAS53360.2.
RefSeq	NP_985536.2. NM_210890.2.
3D structure databases
ProteinModelPortal	Q754T3.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_AFL012C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4621773.
KEGG	ago:AGOS_AFL012C.
Phylogenomic databases
eggNOG	COG1676.
HOGENOM	HOG000175584.
KO	K15323.
OMA	RYVAYPG.
OrthoDB	EOG4WDHN7.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006676. tRNA_splic. IPR016690. tRNA_splic_SEN34. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. [Graphical view]
PIRSF	PIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit.
TIGRFAMs	TIGR00324. endA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SEN34_ASHGO

Accession

Primary (citable) accession number: Q754T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	January 9, 2013
Last modified:	May 1, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program