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Protein

tRNA-splicing endonuclease subunit SEN34

Gene

SEN34

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei202By similarity1
Active sitei210By similarity1
Active sitei243By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit SEN34 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease SEN34
Gene namesi
Name:SEN34
Ordered Locus Names:AFL012C
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
Proteomesi
  • UP000000591 Componenti: Chromosome VI

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094651 – 292tRNA-splicing endonuclease subunit SEN34Add BLAST292

Proteomic databases

PRIDEiQ754T3

Interactioni

Subunit structurei

Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15 (By similarity).By similarity

Protein-protein interaction databases

STRINGi33169.AAS53360

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

HOGENOMiHOG000175584
InParanoidiQ754T3
KOiK15323
OMAiRTFSIEW
OrthoDBiEOG092C3X38

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006676 tRNA_splic
IPR016690 tRNA_splic_SEN34
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PIRSFiPIRSF017250 tRNA_splic_SEN34, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

Q754T3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHTKVAISL NSKTGVPLVF DLDDVRRIRG LGVLGTLCGS LPTAAQQNLF
60 70 80 90 100
LTVPLRLMLE DAVWLVLSGH AYFSFDDKLL VQAAGSLTAA EVCRWRQDTE
110 120 130 140 150
RGLEQQRELR RREQQLKLAA LHRTADETDT QRRLLEQSLF LETENCSKMI
160 170 180 190 200
AAAEPAGLQL AILREMVAQH LDLGNYLIYR YLREEQYFLS AGGRFGARYV
210 220 230 240 250
AYPGDPLRYH SHMAVQPAMD YYNESLDLLH VVGGGRLGTG VKKLWVVGGV
260 270 280 290
RYREAEAAAT AEEQIPPPDT LDRLLATSPP VSVFSIEWSG FG
Length:292
Mass (Da):32,584
Last modified:January 9, 2013 - v2
Checksum:iBFFFBD0B40DC4B6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016819 Genomic DNA Translation: AAS53360.2
RefSeqiNP_985536.2, NM_210890.2

Genome annotation databases

EnsemblFungiiAAS53360; AAS53360; AGOS_AFL012C
GeneIDi4621773
KEGGiago:AGOS_AFL012C

Similar proteinsi

Entry informationi

Entry nameiSEN34_ASHGO
AccessioniPrimary (citable) accession number: Q754T3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 9, 2013
Last modified: May 23, 2018
This is version 92 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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