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Q754T3 (SEN34_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease subunit SEN34

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease SEN34
Gene names
Name:SEN34
Ordered Locus Names:AFL012C
OrganismAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]
Taxonomic identifier284811 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage By similarity.

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Subunit structure

Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15 By similarity.

Sequence similarities

Belongs to the tRNA-intron endonuclease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 292292tRNA-splicing endonuclease subunit SEN34
PRO_0000109465

Sites

Active site2021 By similarity
Active site2101 By similarity
Active site2431 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q754T3 [UniParc].

Last modified January 9, 2013. Version 2.
Checksum: BFFFBD0B40DC4B6A

FASTA29232,584
        10         20         30         40         50         60 
MPHTKVAISL NSKTGVPLVF DLDDVRRIRG LGVLGTLCGS LPTAAQQNLF LTVPLRLMLE 

        70         80         90        100        110        120 
DAVWLVLSGH AYFSFDDKLL VQAAGSLTAA EVCRWRQDTE RGLEQQRELR RREQQLKLAA 

       130        140        150        160        170        180 
LHRTADETDT QRRLLEQSLF LETENCSKMI AAAEPAGLQL AILREMVAQH LDLGNYLIYR 

       190        200        210        220        230        240 
YLREEQYFLS AGGRFGARYV AYPGDPLRYH SHMAVQPAMD YYNESLDLLH VVGGGRLGTG 

       250        260        270        280        290 
VKKLWVVGGV RYREAEAAAT AEEQIPPPDT LDRLLATSPP VSVFSIEWSG FG 

« Hide

References

« Hide 'large scale' references
[1]"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]"Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 51; 53; 70-72; 83-84; 98; 100 AND 102.
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016819 Genomic DNA. Translation: AAS53360.2.
RefSeqNP_985536.2. NM_210890.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING33169.AGOS_AFL012C.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4621773.
KEGGago:AGOS_AFL012C.

Phylogenomic databases

eggNOGCOG1676.
HOGENOMHOG000175584.
KOK15323.
OrthoDBEOG7N905X.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR016690. tRNA_splic_SEN34.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
PIRSFPIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAMSSF53032. SSF53032. 1 hit.
TIGRFAMsTIGR00324. endA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSEN34_ASHGO
AccessionPrimary (citable) accession number: Q754T3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 9, 2013
Last modified: June 11, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families