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Q754T3

- SEN34_ASHGO

UniProt

Q754T3 - SEN34_ASHGO

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Protein
tRNA-splicing endonuclease subunit SEN34
Gene
SEN34, AFL012C
Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage By similarity.

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei202 – 2021 By similarity
Active sitei210 – 2101 By similarity
Active sitei243 – 2431 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: InterPro

GO - Biological processi

  1. tRNA-type intron splice site recognition and cleavage Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit SEN34 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease SEN34
Gene namesi
Name:SEN34
Ordered Locus Names:AFL012C
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
ProteomesiUP000000591: Chromosome VI

Subcellular locationi

GO - Cellular componenti

  1. tRNA-intron endonuclease complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292tRNA-splicing endonuclease subunit SEN34
PRO_0000109465Add
BLAST

Interactioni

Subunit structurei

Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15 By similarity.

Protein-protein interaction databases

STRINGi33169.AGOS_AFL012C.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000175584.
KOiK15323.
OrthoDBiEOG7N905X.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR016690. tRNA_splic_SEN34.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
PIRSFiPIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q754T3-1 [UniParc]FASTAAdd to Basket

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MPHTKVAISL NSKTGVPLVF DLDDVRRIRG LGVLGTLCGS LPTAAQQNLF    50
LTVPLRLMLE DAVWLVLSGH AYFSFDDKLL VQAAGSLTAA EVCRWRQDTE 100
RGLEQQRELR RREQQLKLAA LHRTADETDT QRRLLEQSLF LETENCSKMI 150
AAAEPAGLQL AILREMVAQH LDLGNYLIYR YLREEQYFLS AGGRFGARYV 200
AYPGDPLRYH SHMAVQPAMD YYNESLDLLH VVGGGRLGTG VKKLWVVGGV 250
RYREAEAAAT AEEQIPPPDT LDRLLATSPP VSVFSIEWSG FG 292
Length:292
Mass (Da):32,584
Last modified:January 9, 2013 - v2
Checksum:iBFFFBD0B40DC4B6A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016819 Genomic DNA. Translation: AAS53360.2.
RefSeqiNP_985536.2. NM_210890.2.

Genome annotation databases

GeneIDi4621773.
KEGGiago:AGOS_AFL012C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016819 Genomic DNA. Translation: AAS53360.2 .
RefSeqi NP_985536.2. NM_210890.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 33169.AGOS_AFL012C.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4621773.
KEGGi ago:AGOS_AFL012C.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000175584.
KOi K15323.
OrthoDBi EOG7N905X.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR016690. tRNA_splic_SEN34.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
[Graphical view ]
PIRSFi PIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAMi SSF53032. SSF53032. 1 hit.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
    Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
    Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
  2. "Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
    Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
    G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 51; 53; 70-72; 83-84; 98; 100 AND 102.
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Entry informationi

Entry nameiSEN34_ASHGO
AccessioniPrimary (citable) accession number: Q754T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 9, 2013
Last modified: June 11, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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