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Q754R5 (UBP4_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 4

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene names
Name:DOA4
Synonyms:UBP4
Ordered Locus Names:AFR007W
OrganismAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]
Taxonomic identifier284811 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium

Protein attributes

Sequence length852 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulation

RFU1 is an inhibitor of deubiquitination activity By similarity.

Subcellular location

Cytoplasm By similarity. Late endosome membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 rhodanese domain.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 852852Ubiquitin carboxyl-terminal hydrolase 4
PRO_0000376818

Regions

Domain172 – 296125Rhodanese
Domain488 – 849362USP
Compositional bias370 – 45485Ser-rich

Sites

Active site4971Nucleophile By similarity
Active site8061Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q754R5 [UniParc].

Last modified January 9, 2013. Version 2.
Checksum: 770745A86B229FAD

FASTA85296,523
        10         20         30         40         50         60 
MPHIQESRVW CRSISQLSHL AEQFVTENGT ESTDMKLLLQ QCVDTLSNYQ DECKKIKSVS 

        70         80         90        100        110        120 
KPVPSKELYD LYETAYVYFK IVSLIVLNKI PKLEEYARAK SDAVDRTGKQ LLEIYNMLVN 

       130        140        150        160        170        180 
RLVKDDRIAE IKRFVKENSR RDPEAKNEQI GVESGKSIPA TLLRNLLMGP SASGTVLLVD 

       190        200        210        220        230        240 
VRPRLDFMRC HIKSSSIICI EPVSFKESYT DIDLGKKSMI TSPDAEIALF QDRDKFDYIV 

       250        260        270        280        290        300 
VYTQDSEKNK HNVQQQQLLV DLLINRSFEK ALDRTKVFIL AGGFSEWSNA HPDFCVSSQG 

       310        320        330        340        350        360 
DSVYLNGDTS GLSLQLMPQT TPQKQYNNMF QTMLSGPTDV HGIIRNPHNF PTQQKSKLKR 

       370        380        390        400        410        420 
VPSFRDYFRS SSSSSNINER PGSVPPQLSN GSTIYPETPK LMTNDEYMKS LPQLSPITAR 

       430        440        450        460        470        480 
AITSPSRALS AVGVSKSSAS NSISSLLANS GSASPMKPPD TPLPFTDSIK TLGQQNLTVA 

       490        500        510        520        530        540 
VSNLNFSVGL VNCGNSCYMS CIIQCLLGTQ ELCTMFLNNS YQNHINLNSR LGSKGLLARY 

       550        560        570        580        590        600 
FSQLIHQMYQ YGKDIRKKMG NEKTAVIPTQ FKIACGSINS SFKDNTQQDC QEFCQFLLDG 

       610        620        630        640        650        660 
LHEDLNQCGN NPPLKELSEE AEKMREMMPM RLASAIEWER YLTTDFSVIV DLFQGQYASQ 

       670        680        690        700        710        720 
LQCKVCQRTS TTYQPFSVLS VPVPSTRTCT LTDCFTEFTK IETLEQEEQW SCPSCKKRQP 

       730        740        750        760        770        780 
STKKITITRL PRNLIIHLKR FDNMLNKNNV FVSYPSVLDL TAFWANDYDK KVTNNNVELP 

       790        800        810        820        830        840 
SRGQVPPFNY QLYGIACHDG TLRAGHYTAY VNKGAVLGWC YYDDTNWRQI RSAREYITQN 

       850 
AYVLFYHRIH ST 

« Hide

References

« Hide 'large scale' references
[1]"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]"Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO C-TERMINUS.
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016819 Genomic DNA. Translation: AAS53378.2.
RefSeqNP_985554.2. NM_210908.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING33169.AGOS_AFR007W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4621793.
KEGGago:AGOS_AFR007W.

Phylogenomic databases

eggNOGCOG5533.
HOGENOMHOG000248489.
KOK11839.
OrthoDBEOG7R2BSX.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP4_ASHGO
AccessionPrimary (citable) accession number: Q754R5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: January 9, 2013
Last modified: April 16, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries