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Q754R5

- UBP4_ASHGO

UniProt

Q754R5 - UBP4_ASHGO

Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

DOA4

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (09 Jan 2013)
      Previous versions | rss
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    Functioni

    Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Enzyme regulationi

    RFU1 is an inhibitor of deubiquitination activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei497 – 4971NucleophilePROSITE-ProRule annotation
    Active sitei806 – 8061Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. endopeptidase activity Source: EnsemblFungi
    2. ubiquitin-specific protease activity Source: EnsemblFungi

    GO - Biological processi

    1. endocytosis Source: EnsemblFungi
    2. free ubiquitin chain depolymerization Source: EnsemblFungi
    3. regulation of DNA replication Source: EnsemblFungi
    4. ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: EnsemblFungi
    5. ubiquitin homeostasis Source: EnsemblFungi

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 4
    Ubiquitin thioesterase 4
    Ubiquitin-specific-processing protease 4
    Gene namesi
    Name:DOA4
    Synonyms:UBP4
    Ordered Locus Names:AFR007W
    OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
    Taxonomic identifieri284811 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
    ProteomesiUP000000591: Chromosome VI

    Subcellular locationi

    Cytoplasm By similarity. Late endosome membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. late endosome membrane Source: UniProtKB-SubCell
    2. mitochondrion Source: EnsemblFungi
    3. proteasome complex Source: EnsemblFungi

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 852852Ubiquitin carboxyl-terminal hydrolase 4PRO_0000376818Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi33169.AGOS_AFR007W.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini172 – 296125RhodanesePROSITE-ProRule annotationAdd
    BLAST
    Domaini488 – 849362USPAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi370 – 45485Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5533.
    HOGENOMiHOG000248489.
    KOiK11839.
    OrthoDBiEOG7R2BSX.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q754R5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPHIQESRVW CRSISQLSHL AEQFVTENGT ESTDMKLLLQ QCVDTLSNYQ    50
    DECKKIKSVS KPVPSKELYD LYETAYVYFK IVSLIVLNKI PKLEEYARAK 100
    SDAVDRTGKQ LLEIYNMLVN RLVKDDRIAE IKRFVKENSR RDPEAKNEQI 150
    GVESGKSIPA TLLRNLLMGP SASGTVLLVD VRPRLDFMRC HIKSSSIICI 200
    EPVSFKESYT DIDLGKKSMI TSPDAEIALF QDRDKFDYIV VYTQDSEKNK 250
    HNVQQQQLLV DLLINRSFEK ALDRTKVFIL AGGFSEWSNA HPDFCVSSQG 300
    DSVYLNGDTS GLSLQLMPQT TPQKQYNNMF QTMLSGPTDV HGIIRNPHNF 350
    PTQQKSKLKR VPSFRDYFRS SSSSSNINER PGSVPPQLSN GSTIYPETPK 400
    LMTNDEYMKS LPQLSPITAR AITSPSRALS AVGVSKSSAS NSISSLLANS 450
    GSASPMKPPD TPLPFTDSIK TLGQQNLTVA VSNLNFSVGL VNCGNSCYMS 500
    CIIQCLLGTQ ELCTMFLNNS YQNHINLNSR LGSKGLLARY FSQLIHQMYQ 550
    YGKDIRKKMG NEKTAVIPTQ FKIACGSINS SFKDNTQQDC QEFCQFLLDG 600
    LHEDLNQCGN NPPLKELSEE AEKMREMMPM RLASAIEWER YLTTDFSVIV 650
    DLFQGQYASQ LQCKVCQRTS TTYQPFSVLS VPVPSTRTCT LTDCFTEFTK 700
    IETLEQEEQW SCPSCKKRQP STKKITITRL PRNLIIHLKR FDNMLNKNNV 750
    FVSYPSVLDL TAFWANDYDK KVTNNNVELP SRGQVPPFNY QLYGIACHDG 800
    TLRAGHYTAY VNKGAVLGWC YYDDTNWRQI RSAREYITQN AYVLFYHRIH 850
    ST 852
    Length:852
    Mass (Da):96,523
    Last modified:January 9, 2013 - v2
    Checksum:i770745A86B229FAD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016819 Genomic DNA. Translation: AAS53378.2.
    RefSeqiNP_985554.2. NM_210908.2.

    Genome annotation databases

    GeneIDi4621793.
    KEGGiago:AGOS_AFR007W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016819 Genomic DNA. Translation: AAS53378.2 .
    RefSeqi NP_985554.2. NM_210908.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 33169.AGOS_AFR007W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4621793.
    KEGGi ago:AGOS_AFR007W.

    Phylogenomic databases

    eggNOGi COG5533.
    HOGENOMi HOG000248489.
    KOi K11839.
    OrthoDBi EOG7R2BSX.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00581. Rhodanese. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
      Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
      Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
    2. "Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
      Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
      G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO C-TERMINUS.
      Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

    Entry informationi

    Entry nameiUBP4_ASHGO
    AccessioniPrimary (citable) accession number: Q754R5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: January 9, 2013
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3