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Q754R5

- UBP4_ASHGO

UniProt

Q754R5 - UBP4_ASHGO

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Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene
DOA4, UBP4, AFR007W
Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

RFU1 is an inhibitor of deubiquitination activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei497 – 4971Nucleophile By similarity
Active sitei806 – 8061Proton acceptor By similarity

GO - Molecular functioni

  1. endopeptidase activity Source: EnsemblFungi
  2. ubiquitin-specific protease activity Source: EnsemblFungi

GO - Biological processi

  1. endocytosis Source: EnsemblFungi
  2. free ubiquitin chain depolymerization Source: EnsemblFungi
  3. regulation of DNA replication Source: EnsemblFungi
  4. ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: EnsemblFungi
  5. ubiquitin homeostasis Source: EnsemblFungi
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:DOA4
Synonyms:UBP4
Ordered Locus Names:AFR007W
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
ProteomesiUP000000591: Chromosome VI

Subcellular locationi

GO - Cellular componenti

  1. late endosome membrane Source: UniProtKB-SubCell
  2. mitochondrion Source: EnsemblFungi
  3. proteasome complex Source: EnsemblFungi
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 852852Ubiquitin carboxyl-terminal hydrolase 4PRO_0000376818Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi33169.AGOS_AFR007W.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 296125RhodaneseAdd
BLAST
Domaini488 – 849362USPAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi370 – 45485Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 rhodanese domain.
Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000248489.
KOiK11839.
OrthoDBiEOG7R2BSX.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q754R5-1 [UniParc]FASTAAdd to Basket

« Hide

MPHIQESRVW CRSISQLSHL AEQFVTENGT ESTDMKLLLQ QCVDTLSNYQ    50
DECKKIKSVS KPVPSKELYD LYETAYVYFK IVSLIVLNKI PKLEEYARAK 100
SDAVDRTGKQ LLEIYNMLVN RLVKDDRIAE IKRFVKENSR RDPEAKNEQI 150
GVESGKSIPA TLLRNLLMGP SASGTVLLVD VRPRLDFMRC HIKSSSIICI 200
EPVSFKESYT DIDLGKKSMI TSPDAEIALF QDRDKFDYIV VYTQDSEKNK 250
HNVQQQQLLV DLLINRSFEK ALDRTKVFIL AGGFSEWSNA HPDFCVSSQG 300
DSVYLNGDTS GLSLQLMPQT TPQKQYNNMF QTMLSGPTDV HGIIRNPHNF 350
PTQQKSKLKR VPSFRDYFRS SSSSSNINER PGSVPPQLSN GSTIYPETPK 400
LMTNDEYMKS LPQLSPITAR AITSPSRALS AVGVSKSSAS NSISSLLANS 450
GSASPMKPPD TPLPFTDSIK TLGQQNLTVA VSNLNFSVGL VNCGNSCYMS 500
CIIQCLLGTQ ELCTMFLNNS YQNHINLNSR LGSKGLLARY FSQLIHQMYQ 550
YGKDIRKKMG NEKTAVIPTQ FKIACGSINS SFKDNTQQDC QEFCQFLLDG 600
LHEDLNQCGN NPPLKELSEE AEKMREMMPM RLASAIEWER YLTTDFSVIV 650
DLFQGQYASQ LQCKVCQRTS TTYQPFSVLS VPVPSTRTCT LTDCFTEFTK 700
IETLEQEEQW SCPSCKKRQP STKKITITRL PRNLIIHLKR FDNMLNKNNV 750
FVSYPSVLDL TAFWANDYDK KVTNNNVELP SRGQVPPFNY QLYGIACHDG 800
TLRAGHYTAY VNKGAVLGWC YYDDTNWRQI RSAREYITQN AYVLFYHRIH 850
ST 852
Length:852
Mass (Da):96,523
Last modified:January 9, 2013 - v2
Checksum:i770745A86B229FAD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016819 Genomic DNA. Translation: AAS53378.2.
RefSeqiNP_985554.2. NM_210908.2.

Genome annotation databases

GeneIDi4621793.
KEGGiago:AGOS_AFR007W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016819 Genomic DNA. Translation: AAS53378.2 .
RefSeqi NP_985554.2. NM_210908.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 33169.AGOS_AFR007W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4621793.
KEGGi ago:AGOS_AFR007W.

Phylogenomic databases

eggNOGi COG5533.
HOGENOMi HOG000248489.
KOi K11839.
OrthoDBi EOG7R2BSX.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
    Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
    Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
  2. "Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
    Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
    G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO C-TERMINUS.
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Entry informationi

Entry nameiUBP4_ASHGO
AccessioniPrimary (citable) accession number: Q754R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: January 9, 2013
Last modified: April 16, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi