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Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

DOA4

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

RFU1 is an inhibitor of deubiquitination activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei497NucleophilePROSITE-ProRule annotation1
Active sitei806Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:DOA4
Synonyms:UBP4
Ordered Locus Names:AFR007W
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
Proteomesi
  • UP000000591 Componenti: Chromosome VI

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003768181 – 852Ubiquitin carboxyl-terminal hydrolase 4Add BLAST852

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini172 – 296RhodanesePROSITE-ProRule annotationAdd BLAST125
Domaini488 – 849USPAdd BLAST362

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi370 – 454Ser-richAdd BLAST85

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Phylogenomic databases

HOGENOMiHOG000248489.
InParanoidiQ754R5.
KOiK11839.
OMAiGQYASQL.
OrthoDBiEOG092C5R71.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q754R5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHIQESRVW CRSISQLSHL AEQFVTENGT ESTDMKLLLQ QCVDTLSNYQ
60 70 80 90 100
DECKKIKSVS KPVPSKELYD LYETAYVYFK IVSLIVLNKI PKLEEYARAK
110 120 130 140 150
SDAVDRTGKQ LLEIYNMLVN RLVKDDRIAE IKRFVKENSR RDPEAKNEQI
160 170 180 190 200
GVESGKSIPA TLLRNLLMGP SASGTVLLVD VRPRLDFMRC HIKSSSIICI
210 220 230 240 250
EPVSFKESYT DIDLGKKSMI TSPDAEIALF QDRDKFDYIV VYTQDSEKNK
260 270 280 290 300
HNVQQQQLLV DLLINRSFEK ALDRTKVFIL AGGFSEWSNA HPDFCVSSQG
310 320 330 340 350
DSVYLNGDTS GLSLQLMPQT TPQKQYNNMF QTMLSGPTDV HGIIRNPHNF
360 370 380 390 400
PTQQKSKLKR VPSFRDYFRS SSSSSNINER PGSVPPQLSN GSTIYPETPK
410 420 430 440 450
LMTNDEYMKS LPQLSPITAR AITSPSRALS AVGVSKSSAS NSISSLLANS
460 470 480 490 500
GSASPMKPPD TPLPFTDSIK TLGQQNLTVA VSNLNFSVGL VNCGNSCYMS
510 520 530 540 550
CIIQCLLGTQ ELCTMFLNNS YQNHINLNSR LGSKGLLARY FSQLIHQMYQ
560 570 580 590 600
YGKDIRKKMG NEKTAVIPTQ FKIACGSINS SFKDNTQQDC QEFCQFLLDG
610 620 630 640 650
LHEDLNQCGN NPPLKELSEE AEKMREMMPM RLASAIEWER YLTTDFSVIV
660 670 680 690 700
DLFQGQYASQ LQCKVCQRTS TTYQPFSVLS VPVPSTRTCT LTDCFTEFTK
710 720 730 740 750
IETLEQEEQW SCPSCKKRQP STKKITITRL PRNLIIHLKR FDNMLNKNNV
760 770 780 790 800
FVSYPSVLDL TAFWANDYDK KVTNNNVELP SRGQVPPFNY QLYGIACHDG
810 820 830 840 850
TLRAGHYTAY VNKGAVLGWC YYDDTNWRQI RSAREYITQN AYVLFYHRIH

ST
Length:852
Mass (Da):96,523
Last modified:January 9, 2013 - v2
Checksum:i770745A86B229FAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016819 Genomic DNA. Translation: AAS53378.2.
RefSeqiNP_985554.2. NM_210908.2.

Genome annotation databases

EnsemblFungiiAAS53378; AAS53378; AGOS_AFR007W.
GeneIDi4621793.
KEGGiago:AGOS_AFR007W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016819 Genomic DNA. Translation: AAS53378.2.
RefSeqiNP_985554.2. NM_210908.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC19.005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAAS53378; AAS53378; AGOS_AFR007W.
GeneIDi4621793.
KEGGiago:AGOS_AFR007W.

Phylogenomic databases

HOGENOMiHOG000248489.
InParanoidiQ754R5.
KOiK11839.
OMAiGQYASQL.
OrthoDBiEOG092C5R71.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP4_ASHGO
AccessioniPrimary (citable) accession number: Q754R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: January 9, 2013
Last modified: September 7, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.