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Q754R5

- UBP4_ASHGO

UniProt

Q754R5 - UBP4_ASHGO

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Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

DOA4

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

RFU1 is an inhibitor of deubiquitination activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei497 – 4971NucleophilePROSITE-ProRule annotation
Active sitei806 – 8061Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. endopeptidase activity Source: EnsemblFungi
  2. ubiquitin-specific protease activity Source: EnsemblFungi

GO - Biological processi

  1. endocytosis Source: EnsemblFungi
  2. free ubiquitin chain depolymerization Source: EnsemblFungi
  3. regulation of DNA replication Source: EnsemblFungi
  4. ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: EnsemblFungi
  5. ubiquitin homeostasis Source: EnsemblFungi
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:DOA4
Synonyms:UBP4
Ordered Locus Names:AFR007W
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
ProteomesiUP000000591: Chromosome VI

Subcellular locationi

Cytoplasm By similarity. Late endosome membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. endosome Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
  3. mitochondrion Source: EnsemblFungi
  4. proteasome complex Source: EnsemblFungi
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 852852Ubiquitin carboxyl-terminal hydrolase 4PRO_0000376818Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi33169.AGOS_AFR007W.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 296125RhodanesePROSITE-ProRule annotationAdd
BLAST
Domaini488 – 849362USPAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi370 – 45485Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000248489.
InParanoidiQ754R5.
KOiK11839.
OrthoDBiEOG7R2BSX.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q754R5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPHIQESRVW CRSISQLSHL AEQFVTENGT ESTDMKLLLQ QCVDTLSNYQ
60 70 80 90 100
DECKKIKSVS KPVPSKELYD LYETAYVYFK IVSLIVLNKI PKLEEYARAK
110 120 130 140 150
SDAVDRTGKQ LLEIYNMLVN RLVKDDRIAE IKRFVKENSR RDPEAKNEQI
160 170 180 190 200
GVESGKSIPA TLLRNLLMGP SASGTVLLVD VRPRLDFMRC HIKSSSIICI
210 220 230 240 250
EPVSFKESYT DIDLGKKSMI TSPDAEIALF QDRDKFDYIV VYTQDSEKNK
260 270 280 290 300
HNVQQQQLLV DLLINRSFEK ALDRTKVFIL AGGFSEWSNA HPDFCVSSQG
310 320 330 340 350
DSVYLNGDTS GLSLQLMPQT TPQKQYNNMF QTMLSGPTDV HGIIRNPHNF
360 370 380 390 400
PTQQKSKLKR VPSFRDYFRS SSSSSNINER PGSVPPQLSN GSTIYPETPK
410 420 430 440 450
LMTNDEYMKS LPQLSPITAR AITSPSRALS AVGVSKSSAS NSISSLLANS
460 470 480 490 500
GSASPMKPPD TPLPFTDSIK TLGQQNLTVA VSNLNFSVGL VNCGNSCYMS
510 520 530 540 550
CIIQCLLGTQ ELCTMFLNNS YQNHINLNSR LGSKGLLARY FSQLIHQMYQ
560 570 580 590 600
YGKDIRKKMG NEKTAVIPTQ FKIACGSINS SFKDNTQQDC QEFCQFLLDG
610 620 630 640 650
LHEDLNQCGN NPPLKELSEE AEKMREMMPM RLASAIEWER YLTTDFSVIV
660 670 680 690 700
DLFQGQYASQ LQCKVCQRTS TTYQPFSVLS VPVPSTRTCT LTDCFTEFTK
710 720 730 740 750
IETLEQEEQW SCPSCKKRQP STKKITITRL PRNLIIHLKR FDNMLNKNNV
760 770 780 790 800
FVSYPSVLDL TAFWANDYDK KVTNNNVELP SRGQVPPFNY QLYGIACHDG
810 820 830 840 850
TLRAGHYTAY VNKGAVLGWC YYDDTNWRQI RSAREYITQN AYVLFYHRIH

ST
Length:852
Mass (Da):96,523
Last modified:January 9, 2013 - v2
Checksum:i770745A86B229FAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016819 Genomic DNA. Translation: AAS53378.2.
RefSeqiNP_985554.2. NM_210908.2.

Genome annotation databases

GeneIDi4621793.
KEGGiago:AGOS_AFR007W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016819 Genomic DNA. Translation: AAS53378.2 .
RefSeqi NP_985554.2. NM_210908.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 33169.AGOS_AFR007W.

Protein family/group databases

MEROPSi C19.005.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4621793.
KEGGi ago:AGOS_AFR007W.

Phylogenomic databases

eggNOGi COG5533.
HOGENOMi HOG000248489.
InParanoidi Q754R5.
KOi K11839.
OrthoDBi EOG7R2BSX.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
    Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
    Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
  2. "Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
    Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
    G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO C-TERMINUS.
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Entry informationi

Entry nameiUBP4_ASHGO
AccessioniPrimary (citable) accession number: Q754R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: January 9, 2013
Last modified: October 29, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3