Reviewed, UniProtKB/Swiss-Prot Q753X4 (PMIP_ASHGO)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
Ordered Locus Names:	AFR198W

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 28	28	Mitochondrion Potential
	Chain	29 – 776	748	Mitochondrial intermediate peptidase		PRO_0000338568
Sites
	Active site	568	1	By similarity
	Metal binding	567	1	Zinc; catalytic By similarity
	Metal binding	571	1	Zinc; catalytic By similarity
	Metal binding	574	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q753X4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 78E4201F03A2B72F	FASTA	776	88,597
	10 20 30 40 50 60 MFVRFYKRLD RQYIQSQRRW ILSSNKCLLQ GNKNATISPL RRAFDDQDHW EESQAQNTSS 70 80 90 100 110 120 EQDNKGKNSS YFWSRSKATA PQVAHTGLFQ NPYLNSPEGL RKFANKSLTE ATALVRNLRE 130 140 150 160 170 180 DSSQEGLVRY IIRLDQLSDI LCRVIDLCEF LRAAHPDEQF VAAAQECHEQ MFEIMNILNT 190 200 210 220 230 240 DVVLCKRLKQ VLSDENISSK LSSEEIRVGH ILLEDFEKAG AYASPEVRKQ FIQLSQNISI 250 260 270 280 290 300 IGQDFINNTE SLSSSYIKIP CKDLESSGTS HLVLRQLTKD TMGNNYKIPT SGYAPYTLLN 310 320 330 340 350 360 ACPSEAIRRQ VWTAMFSCSE KQVKRLKSLL QLRRKLANIM GATDYVSYQL EGKMAKSPEN 370 380 390 400 410 420 VKNFLNTLVD HTKPLAAGEL EELAKLKRNV ENLSETNTLK LMRPWDRDYY SSLSPNFTRP 430 440 450 460 470 480 NHRVDGFTSI NTYFSLGVVM QGISDLFRDI YGISLKPVVA QAGETWAPDV RKLQVISETE 490 500 510 520 530 540 GIIGLIYCDL LERPGKTTSP SHFTVCCSRQ IYPEENDFST IQVGENPDGS RFQMPVISLI 550 560 570 580 590 600 CNFRATRHGK NKSLCLLELS DVETLFHEMG HALHSMLGRT QLQNLSGTRC VTDFVELPSI 610 620 630 640 650 660 LMEHFAKDRR VLLRISSNYA TGEPIPEELL SAFQEQNNFL KNTETFSQIK MSMLDQRLHS 670 680 690 700 710 720 ITDQDDIIAV YHGLEREMEV LVDDQTNWCG RFGHLFGYGA SYYSYLMDRA IAAKIWDHLF 730 740 750 760 770 KKDPFSRSSG EKFKEGVLKW GGSRDAWQCI ADALDEPRLV KGDDWAMRFI GEVEDM

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016819 Genomic DNA. Translation: AAS53569.1.
RefSeq	NP_985745.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q753X4.
Protein family/group databases
MEROPS	M03.006.
Genome annotation databases
GeneID	4622005.
GenomeReviews	Gene locus AFR198W in contig AE016819_GR.
KEGG	ago:AGOS_AFR198W.
NMPDR	fig|33169.1.peg.3390.
Organism-specific databases
AGD	AFR198W.
Phylogenomic databases
HOGENOM	Q753X4.
OMA	VTEMNIE.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_ASHGO

Accession

Primary (citable) accession number: Q753X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)