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Protein

Mitochondrial intermediate peptidase

Gene

OCT1

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane (By similarity).By similarity

Catalytic activityi

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi567 – 5671Zinc; catalyticPROSITE-ProRule annotation
Active sitei568 – 5681PROSITE-ProRule annotation
Metal bindingi571 – 5711Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi574 – 5741Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial intermediate peptidase (EC:3.4.24.59)
Short name:
MIP
Alternative name(s):
Octapeptidyl aminopeptidase
Gene namesi
Name:OCT1
Ordered Locus Names:AFR198W
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
ProteomesiUP000000591 Componenti: Chromosome VI

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2828MitochondrionSequence AnalysisAdd
BLAST
Chaini29 – 776748Mitochondrial intermediate peptidasePRO_0000338568Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ753X4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0339.
HOGENOMiHOG000076521.
InParanoidiQ753X4.
KOiK01410.
OMAiIIIDGLH.
OrthoDBiEOG71GB4R.

Family and domain databases

Gene3Di1.10.1370.10. 2 hits.
3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q753X4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFVRFYKRLD RQYIQSQRRW ILSSNKCLLQ GNKNATISPL RRAFDDQDHW
60 70 80 90 100
EESQAQNTSS EQDNKGKNSS YFWSRSKATA PQVAHTGLFQ NPYLNSPEGL
110 120 130 140 150
RKFANKSLTE ATALVRNLRE DSSQEGLVRY IIRLDQLSDI LCRVIDLCEF
160 170 180 190 200
LRAAHPDEQF VAAAQECHEQ MFEIMNILNT DVVLCKRLKQ VLSDENISSK
210 220 230 240 250
LSSEEIRVGH ILLEDFEKAG AYASPEVRKQ FIQLSQNISI IGQDFINNTE
260 270 280 290 300
SLSSSYIKIP CKDLESSGTS HLVLRQLTKD TMGNNYKIPT SGYAPYTLLN
310 320 330 340 350
ACPSEAIRRQ VWTAMFSCSE KQVKRLKSLL QLRRKLANIM GATDYVSYQL
360 370 380 390 400
EGKMAKSPEN VKNFLNTLVD HTKPLAAGEL EELAKLKRNV ENLSETNTLK
410 420 430 440 450
LMRPWDRDYY SSLSPNFTRP NHRVDGFTSI NTYFSLGVVM QGISDLFRDI
460 470 480 490 500
YGISLKPVVA QAGETWAPDV RKLQVISETE GIIGLIYCDL LERPGKTTSP
510 520 530 540 550
SHFTVCCSRQ IYPEENDFST IQVGENPDGS RFQMPVISLI CNFRATRHGK
560 570 580 590 600
NKSLCLLELS DVETLFHEMG HALHSMLGRT QLQNLSGTRC VTDFVELPSI
610 620 630 640 650
LMEHFAKDRR VLLRISSNYA TGEPIPEELL SAFQEQNNFL KNTETFSQIK
660 670 680 690 700
MSMLDQRLHS ITDQDDIIAV YHGLEREMEV LVDDQTNWCG RFGHLFGYGA
710 720 730 740 750
SYYSYLMDRA IAAKIWDHLF KKDPFSRSSG EKFKEGVLKW GGSRDAWQCI
760 770
ADALDEPRLV KGDDWAMRFI GEVEDM
Length:776
Mass (Da):88,597
Last modified:July 5, 2004 - v1
Checksum:i78E4201F03A2B72F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016819 Genomic DNA. Translation: AAS53569.1.
RefSeqiNP_985745.1. NM_211099.1.

Genome annotation databases

EnsemblFungiiAAS53569; AAS53569; AGOS_AFR198W.
GeneIDi4622005.
KEGGiago:AGOS_AFR198W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016819 Genomic DNA. Translation: AAS53569.1.
RefSeqiNP_985745.1. NM_211099.1.

3D structure databases

ProteinModelPortaliQ753X4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAAS53569; AAS53569; AGOS_AFR198W.
GeneIDi4622005.
KEGGiago:AGOS_AFR198W.

Phylogenomic databases

eggNOGiCOG0339.
HOGENOMiHOG000076521.
InParanoidiQ753X4.
KOiK01410.
OMAiIIIDGLH.
OrthoDBiEOG71GB4R.

Family and domain databases

Gene3Di1.10.1370.10. 2 hits.
3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
    Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
    Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
  2. "Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
    Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
    G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Entry informationi

Entry nameiPMIP_ASHGO
AccessioniPrimary (citable) accession number: Q753X4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.