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Q753X4 (PMIP_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial intermediate peptidase

Short name=MIP
EC=3.4.24.59
Alternative name(s):
Octapeptidyl aminopeptidase
Gene names
Name:OCT1
Ordered Locus Names:AFR198W
OrganismAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]
Taxonomic identifier284811 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium

Protein attributes

Sequence length776 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.

Catalytic activity

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactor

Binds 1 zinc ion By similarity.

Subcellular location

Mitochondrion matrix By similarity.

Sequence similarities

Belongs to the peptidase M3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2828Mitochondrion Potential
Chain29 – 776748Mitochondrial intermediate peptidase
PRO_0000338568

Sites

Active site5681 By similarity
Metal binding5671Zinc; catalytic By similarity
Metal binding5711Zinc; catalytic By similarity
Metal binding5741Zinc; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q753X4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 78E4201F03A2B72F

FASTA77688,597
        10         20         30         40         50         60 
MFVRFYKRLD RQYIQSQRRW ILSSNKCLLQ GNKNATISPL RRAFDDQDHW EESQAQNTSS 

        70         80         90        100        110        120 
EQDNKGKNSS YFWSRSKATA PQVAHTGLFQ NPYLNSPEGL RKFANKSLTE ATALVRNLRE 

       130        140        150        160        170        180 
DSSQEGLVRY IIRLDQLSDI LCRVIDLCEF LRAAHPDEQF VAAAQECHEQ MFEIMNILNT 

       190        200        210        220        230        240 
DVVLCKRLKQ VLSDENISSK LSSEEIRVGH ILLEDFEKAG AYASPEVRKQ FIQLSQNISI 

       250        260        270        280        290        300 
IGQDFINNTE SLSSSYIKIP CKDLESSGTS HLVLRQLTKD TMGNNYKIPT SGYAPYTLLN 

       310        320        330        340        350        360 
ACPSEAIRRQ VWTAMFSCSE KQVKRLKSLL QLRRKLANIM GATDYVSYQL EGKMAKSPEN 

       370        380        390        400        410        420 
VKNFLNTLVD HTKPLAAGEL EELAKLKRNV ENLSETNTLK LMRPWDRDYY SSLSPNFTRP 

       430        440        450        460        470        480 
NHRVDGFTSI NTYFSLGVVM QGISDLFRDI YGISLKPVVA QAGETWAPDV RKLQVISETE 

       490        500        510        520        530        540 
GIIGLIYCDL LERPGKTTSP SHFTVCCSRQ IYPEENDFST IQVGENPDGS RFQMPVISLI 

       550        560        570        580        590        600 
CNFRATRHGK NKSLCLLELS DVETLFHEMG HALHSMLGRT QLQNLSGTRC VTDFVELPSI 

       610        620        630        640        650        660 
LMEHFAKDRR VLLRISSNYA TGEPIPEELL SAFQEQNNFL KNTETFSQIK MSMLDQRLHS 

       670        680        690        700        710        720 
ITDQDDIIAV YHGLEREMEV LVDDQTNWCG RFGHLFGYGA SYYSYLMDRA IAAKIWDHLF 

       730        740        750        760        770 
KKDPFSRSSG EKFKEGVLKW GGSRDAWQCI ADALDEPRLV KGDDWAMRFI GEVEDM 

« Hide

References

« Hide 'large scale' references
[1]"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]"Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016819 Genomic DNA. Translation: AAS53569.1.
RefSeqNP_985745.1. NM_211099.1.

3D structure databases

ProteinModelPortalQ753X4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING33169.AGOS_AFR198W.

Protein family/group databases

MEROPSM03.006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiAAS53569; AAS53569; AGOS_AFR198W.
GeneID4622005.
KEGGago:AGOS_AFR198W.

Phylogenomic databases

eggNOGCOG0339.
HOGENOMHOG000076521.
KOK01410.
OMALQVFYSA.
OrthoDBEOG71GB4R.

Family and domain databases

Gene3D1.10.1370.10. 2 hits.
3.40.390.10. 1 hit.
InterProIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePMIP_ASHGO
AccessionPrimary (citable) accession number: Q753X4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries