Reviewed, UniProtKB/Swiss-Prot Q752X9 (FDFT_ASHGO)

Names and origin

Protein names

Recommended name:
    Squalene synthetase
      Short name=SQS
      Short name=SS
    EC=2.5.1.21
Alternative name(s):
    Farnesyl-diphosphate farnesyltransferase
    FPP:FPP farnesyltransferase

Gene names

Name:	ERG9
Ordered Locus Names:	AFR444C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 farnesyl diphosphate = diphosphate + presqualene diphosphate. Presqualene diphosphate + NAD(P)H = squalene + diphosphate + NAD(P)+.
Cofactor	Magnesium.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the phytoene/squalene synthetase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Isoprene biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Magnesium NADP
Molecular function	Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	farnesyl-diphosphate farnesyltransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Squalene synthetase		PRO_0000067446
Regions
	Transmembrane	293 – 313	21	Potential
	Transmembrane	420 – 440	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q752X9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 53807044FB95195B	FASTA	441	50,873
	10 20 30 40 50 60 MGKVVQLFTH PLELKAALKL KFLREPLYPA DDTQGSAELK RCYQLLQRTS RSFAAVIMEL 70 80 90 100 110 120 HPELRNAVML FYLILRALDT VEDDMTISPK VKVPLLREFD QKLKLDTWSF DGNAKTEKDR 130 140 150 160 170 180 DVLVEFSTIL AEFHKLKPEY QQVIADITHK MGNGMADYIL DEKFNLSGLE TIQDYDRYCH 190 200 210 220 230 240 YVAGLVGDGL THLIMLAKFS SPGLYYDSPD LYESMGLFLQ KTNIIRDYAE DLADGRSFWP 250 260 270 280 290 300 KEIWSHYADD LASFSKPENA TAGVYCINHL VLNALGHVQH VLTYLASLRE QSSFQFCAIP 310 320 330 340 350 360 QVMAIATLAL VFGNERVLQT SVKIRKGTTC YLILKSRTFQ GCVEIFEHYL RDIRKRLTVA 370 380 390 400 410 420 DPNYLKLNIE IAKLDKFIEE MYQDKLPVGA KPQETEIYKK VRERSAYDLE VLPREQEEEF 430 440 KFNVLLSILF TVFGALYWYA K

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016819 Genomic DNA. Translation: AAS53815.1.
RefSeq	NP_985991.1.
3D structure databases
SMR	Q752X9. Positions 38-374.
ModBase	Search...
Protein-protein interaction databases
STRING	Q752X9.
Genome annotation databases
GeneID	4622268.
GenomeReviews	Gene locus AFR444C in contig AE016819_GR.
KEGG	ago:AGOS_AFR444C.
NMPDR	fig|33169.1.peg.3636.
Organism-specific databases
AGD	AFR444C.
Phylogenomic databases
eggNOG	fuNOG05680.
HOGENOM	HBG600510.
OMA	RSFWPKE.
OrthoDB	EOG9BZPJW.
Enzyme and pathway databases
BRENDA	2.5.1.21. 279361.
Family and domain databases
InterPro	IPR002060. Squ/phyt_synthse. IPR006449. Squal_synth. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
TIGRFAMs	TIGR01559. squal_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDFT_ASHGO

Accession

Primary (citable) accession number: Q752X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)