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Protein

Squalene synthase

Gene

ERG9

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol (By similarity).By similarity

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactori

Mg2+By similarity

Pathwayi: lanosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (ERG9)
  2. Squalene monooxygenase (ERG1)
  3. no protein annotated in this organism
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Transferase
Biological processIsoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandMagnesium, NADP

Enzyme and pathway databases

UniPathwayiUPA00767; UER00751

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase (EC:2.5.1.21)
Short name:
SQS
Short name:
SS
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene namesi
Name:ERG9
Ordered Locus Names:AFR444C
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
Proteomesi
  • UP000000591 Componenti: Chromosome VI

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
Transmembranei420 – 440HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000674461 – 441Squalene synthaseAdd BLAST441

Interactioni

Protein-protein interaction databases

STRINGi33169.AAS53815

Structurei

3D structure databases

ProteinModelPortaliQ752X9
SMRiQ752X9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000186940
InParanoidiQ752X9
KOiK00801
OMAiFNFVAIP
OrthoDBiEOG092C2IU6

Family and domain databases

CDDicd00683 Trans_IPPS_HH, 1 hit
Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR002060 Squ/phyt_synthse
IPR006449 Squal_synth-like
IPR019845 Squalene/phytoene_synthase_CS
IPR033904 Trans_IPPS_HH
PfamiView protein in Pfam
PF00494 SQS_PSY, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
TIGRFAMsiTIGR01559 squal_synth, 1 hit
PROSITEiView protein in PROSITE
PS01044 SQUALEN_PHYTOEN_SYN_1, 1 hit
PS01045 SQUALEN_PHYTOEN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q752X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKVVQLFTH PLELKAALKL KFLREPLYPA DDTQGSAELK RCYQLLQRTS
60 70 80 90 100
RSFAAVIMEL HPELRNAVML FYLILRALDT VEDDMTISPK VKVPLLREFD
110 120 130 140 150
QKLKLDTWSF DGNAKTEKDR DVLVEFSTIL AEFHKLKPEY QQVIADITHK
160 170 180 190 200
MGNGMADYIL DEKFNLSGLE TIQDYDRYCH YVAGLVGDGL THLIMLAKFS
210 220 230 240 250
SPGLYYDSPD LYESMGLFLQ KTNIIRDYAE DLADGRSFWP KEIWSHYADD
260 270 280 290 300
LASFSKPENA TAGVYCINHL VLNALGHVQH VLTYLASLRE QSSFQFCAIP
310 320 330 340 350
QVMAIATLAL VFGNERVLQT SVKIRKGTTC YLILKSRTFQ GCVEIFEHYL
360 370 380 390 400
RDIRKRLTVA DPNYLKLNIE IAKLDKFIEE MYQDKLPVGA KPQETEIYKK
410 420 430 440
VRERSAYDLE VLPREQEEEF KFNVLLSILF TVFGALYWYA K
Length:441
Mass (Da):50,873
Last modified:July 5, 2004 - v1
Checksum:i53807044FB95195B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016819 Genomic DNA Translation: AAS53815.1
RefSeqiNP_985991.1, NM_211346.1

Genome annotation databases

EnsemblFungiiAAS53815; AAS53815; AGOS_AFR444C
GeneIDi4622268
KEGGiago:AGOS_AFR444C

Similar proteinsi

Entry informationi

Entry nameiFDFT_ASHGO
AccessioniPrimary (citable) accession number: Q752X9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health