Q751Z4 (ATG26_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sterol 3-beta-glucosyltransferase
EC=2.4.1.173

Alternative name(s):
Autophagy-related protein 26

Gene names

Name:	ATG26
Ordered Locus Names:	AFR681W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	1227 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Cytoplasm Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1227	1227	Sterol 3-beta-glucosyltransferase		PRO_0000215606
Regions
	Domain	188 – 229	42	GRAM 1
	Domain	239 – 342	104	PH
	Domain	602 – 668	67	GRAM 2
	Compositional bias	436 – 491	56	Ala-rich

Sequences

	Sequence		Length	Mass (Da)
	Q751Z4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1D1CD63468C245EA	FASTA	1,227	136,413
	10 20 30 40 50 60 MLKGQKEGEA EPVPEGDKSG AGSSPSGVRK GLRVPLGLVS HSFSSLAKHK EAERRLTDEE 70 80 90 100 110 120 ESLSSPQDRE DDAASPNIMA KSIAGLLTTA SMYVGIGDIH RAEQLATGQP ETDAGADETE 130 140 150 160 170 180 DEMDEAGDET GDDADAPATG TDGEEESRPV SAQESRRSTL FELSIEPHPE SHTAQASRTK 190 200 210 220 230 240 NRRSRMTSKL RSKFNLDDDE ELVREYPCWL LRDVLIQGHI YLTSRNLLFF AFLYKSNGSA 250 260 270 280 290 300 RLTGNLSICN NGLSISGISG KPTRYWTVLK DHTLSLYSSS TDLYFPVLTI DLRYVTKVQH 310 320 330 340 350 360 CKNNGKDTRQ FHITTESKTY TFYSDNEHSA RSWSSALKKQ VFATQNSDND SMTVRIPLSN 370 380 390 400 410 420 IIDVEEQAIV EQGLTLRVRV MESSDSFALD DYFFMFFNNA GSQLKELIQI QVANLEMLGA 430 440 450 460 470 480 ANVDYARHPL PPDTEASLPA VASDAAPQDA AIASEAAADA AAADTASHSP STNAEPRARG 490 500 510 520 530 540 RSTERAMAAS AYLFGRLTSP RRDDAKQLSP SKVKLRFRSV ADTLKLTTPR QPGSDAPQEP 550 560 570 580 590 600 EPETTILESR PRLNYWSPRP FTTMRSMWNA QPVHYAAKGM SLFADDDELM IGDEAELLAA 610 620 630 640 650 660 DKRFKAHFSL TDDESLVASY YTYLNRSMPL YGKIYLGKTI MCFRSLLPGS KTKMILPLHD 670 680 690 700 710 720 VENCYKEQGF RFGYFGLVVV IYGHEELFFE FASQKSRDDA EYVILKIIDS LKPVDGVMAD 730 740 750 760 770 780 DMSAGAYSLK AAQGRDATTD AKVKLFEQRI SSVGYDIPIM VEDNPFYKTT ITPKKFYTFG 790 800 810 820 830 840 MLTIGSRGDV QPYIALGKGL LQEGHRVVVI SHAEFGDWVR SHGLQFRAIA GDPAELMALM 850 860 870 880 890 900 VQHGSMNVGL IREAASTFRN WIRDLLETAW EACQGIDVLI ESPSAMAGIH IAEALQIPYF 910 920 930 940 950 960 RAFTMPWTKT RSYPHAFIVP DQKRGGNYNY FTHVLFENIF WKGINSQVNR WRVEKLGLKK 970 980 990 1000 1010 1020 TNLEFMQQGK VPFLYNMSPT VFPPSVDFAE WIKVTGYWFL NESSNYVPPQ ALLEFMAKAR 1030 1040 1050 1060 1070 1080 RLDKKLVYIG FGSIVVKDPV KMTMAVVEAV VKADVYCILN KGWSARLGGQ SQKSIEVQLP 1090 1100 1110 1120 1130 1140 NCVYDAGNVP HDWLFPRVDA AVHHGGSGTT GATMRAGVPT VIKPFFGDQY FYANRIEDIG 1150 1160 1170 1180 1190 1200 AGIALRKLNA CTLSRALKEV TTNTRIIAKA KKIGQDISKE DGVATAIAFI YSEMAYAKSL 1210 1220 IKAKRQEDKK AAKEAKQIQN EDSWLLL

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016819 Genomic DNA. Translation: AAS54053.1.
RefSeq	NP_986229.2. NM_212365.2.
3D structure databases
ProteinModelPortal	Q751Z4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q751Z4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4622518.
GenomeReviews	Gene locus AFR681W in contig AE016819_GR.
KEGG	ago:AGOS_AFR681W.
NMPDR	fig|33169.1.peg.3874.
Organism-specific databases
AGD	AFR681W.
Phylogenomic databases
eggNOG	fuNOG07241.
HOGENOM	HBG332791.
OMA	RGHEELF.
OrthoDB	EOG48KVK8.
PhylomeDB	Q751Z4.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002213. UDP_glucos_trans. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
KO	K05841.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. PF00201. UDPGT. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_ASHGO

Accession

Primary (citable) accession number: Q751Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program