Q751K5 (ALG3_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
EC=2.4.1.258

Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase

Gene names

Name:	ALG3
Ordered Locus Names:	AGL299C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol By similarity.
Catalytic activity	Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG3 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase		PRO_0000350919
Regions
	Topological domain	1 – 124	124	Lumenal Potential
	Transmembrane	125 – 145	21	Helical; Potential
	Topological domain	146 – 168	23	Cytoplasmic Potential
	Transmembrane	169 – 189	21	Helical; Potential
	Topological domain	190 – 206	17	Lumenal Potential
	Transmembrane	207 – 227	21	Helical; Potential
	Topological domain	228 – 230	3	Cytoplasmic Potential
	Transmembrane	231 – 251	21	Helical; Potential
	Topological domain	252 – 290	39	Lumenal Potential
	Transmembrane	291 – 311	21	Helical; Potential
	Topological domain	312 – 343	32	Cytoplasmic Potential
	Transmembrane	344 – 364	21	Helical; Potential
	Topological domain	365 – 375	11	Lumenal Potential
	Transmembrane	376 – 396	21	Helical; Potential
	Topological domain	397 – 408	12	Cytoplasmic Potential
	Transmembrane	409 – 429	21	Helical; Potential
	Topological domain	430 – 432	3	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q751K5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B462531EC4FC7313	FASTA	432	48,660
	10 20 30 40 50 60 MSAAVPTTRA GTPPTLQCGW RSACGKLLDI ANYVIFSPEA SAVVMPVLVA WECVLLKLIV 70 80 90 100 110 120 KHVPYTEIDY LAYMEQIWQI NNGERDYSKI EGGTGPLVYP AGHVLIHRLL ERATDGLQNV 130 140 150 160 170 180 ARGQDIFTWL YLLTLVLQFG VYRMLRLPPW CIVLACLSKR LHSVYVLRLF NDGWTTLMVV 190 200 210 220 230 240 VAVFLLLLAA RHPRLCLPAA LVYSAAVSIK MNALLYLPGV LVALFLLTRG HLLALALCGA 250 260 270 280 290 300 VAVAWQVLVA ADFLSTHPAE YFATAFDFRR QFMYRWSVNW QLVGEQVFSH PTFHRCLLLS 310 320 330 340 350 360 HIAILMLFFF TRYAEPRQPN WFRTAAAALR HPATAVLAAS PPRAHVAYVL LVSNFIGVLF 370 380 390 400 410 420 ARSLHYQFLA WYHWTLPALL HWARMPCLLA LLWYVLHELC WDTYPPSSVA SATLYALNSA 430 LLLLLYINGP PA

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016820 Genomic DNA. Translation: AAS54192.1.
RefSeq	NP_986368.2. NM_211430.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q751K5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4622661.
GenomeReviews	Gene locus AGL299C in contig AE016820_GR.
KEGG	ago:AGOS_AGL299C.
NMPDR	fig|33169.1.peg.4013.
Organism-specific databases
AGD	AGL299C.
Phylogenomic databases
eggNOG	fuNOG07998.
HOGENOM	HBG525447.
OMA	IHRVAYT.
OrthoDB	EOG4MPN04.
PhylomeDB	Q751K5.
Family and domain databases
InterPro	IPR007873. Glycosyltransferase_ALG3. [Graphical view]
KO	K03845.
PANTHER	PTHR12646. ALG3. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG3_ASHGO

Accession

Primary (citable) accession number: Q751K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program