Reviewed, UniProtKB/Swiss-Prot Q751I2 (KMO_ASHGO)

Names and origin

Protein names

Recommended name:
    Kynurenine 3-monooxygenase
    EC=1.14.13.9
Alternative name(s):
    Kynurenine 3-hydroxylase
    Biosynthesis of nicotinic acid protein 4

Gene names

Name:	BNA4
Ordered Locus Names:	AGL276W

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid By similarity.
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kynurenine 3-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Kynurenine 3-monooxygenase		PRO_0000361915
Amino acid modifications
	Modified residue	7	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q751I2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5D79B03EA4BE8D9B	FASTA	466	52,142
	10 20 30 40 50 60 MGEKGESVAV IGAGLVGCLA ALAFAKKGYE VSLFDYRSDP RLATTTDRNL RSINLAISAR 70 80 90 100 110 120 GIEGLKAVDD ELAARVLRDM LPMHGRMIHN LAGKQEPQEY GLFGESVNSI DRGVLNNALL 130 140 150 160 170 180 DEVSAQEHIQ AQFGHKLVKA NFNHGATQQL LFAVEGKTVQ LEFDFVVGCD GAYSTTRQQM 190 200 210 220 230 240 QRFDRMDFSQ EYMDCFYLEL YIPPTPEFSE RFGGPFAISP QHLHIWPRHN FMLIALPNKD 250 260 270 280 290 300 GSFTSTFFGP WSLLDRLDTR EQLAAFLTTN FADAMELIGL DNAIRAFQEN TKGALMCVEC 310 320 330 340 350 360 RPYHLPGGKA ILLGDAAHAM VPFYGQGMNC GFEDVRVLMG LLDDYAGDRT AAFAKYTASR 370 380 390 400 410 420 HRDLVSIIQL AKNNYRDMSH NVVSSWHRAK RSLNNVLGRT FRGTWLPLYT MVSFRADIPY 430 440 450 460 HKAVEVDRRQ AAILSLVQSA LLSLAALGGF KGLLLLYRWI KQVRRV

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016820 Genomic DNA. Translation: AAS54215.1.
RefSeq	NP_986391.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q751I2.
Genome annotation databases
GeneID	4622684.
GenomeReviews	Gene locus AGL276W in contig AE016820_GR.
KEGG	ago:AGOS_AGL276W.
NMPDR	fig|33169.1.peg.4036.
Organism-specific databases
AGD	AGL276W.
Phylogenomic databases
HOGENOM	Q751I2.
OMA	LHAIMPS.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_ASHGO

Accession

Primary (citable) accession number: Q751I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)