Serine/threonine-protein kinase SSN3 - Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast)

Contribute

Send feedback

Read comments (?) or add your own

Q751F5 (SSN3_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 78. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase SSN3
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cyclin-dependent kinase 8

Gene names

Name:	SSN3
Synonyms:	CDK8
Ordered Locus Names:	AGL249C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium ›

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Component of the SRB8-11 complex, a regulatory module of the Mediator complex By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Activator Kinase Repressor Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 581

581

Serine/threonine-protein kinase SSN3

PRO_0000312932

Regions

Domain

85 – 475

391

Protein kinase

Nucleotide binding

91 – 99

ATP By similarity

Compositional bias

37 – 42

Poly-Gln

Compositional bias

549 – 557

Poly-Ala

Compositional bias

567 – 572

Poly-Ser

Sites

Active site

298

Proton acceptor By similarity

Binding site

195

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q751F5 [UniParc].

Last modified January 9, 2013. Version 2.
Checksum: 11B88D39F280B640
Show »

FASTA

581

64,530

        10         20         30         40         50         60 
MGIRLQSSDS MNNQHQTQQN KYFNVSNQAS TRSLWSQQQQ QQLLDTKGSA STSKSPMLMA 

        70         80         90        100        110        120 
NNNVFSIGPY RQRKDAGRVS VLEKYEIIGY IAAGTYGKVY KAKAKETQEE QENSIHTHNQ 

       130        140        150        160        170        180 
TLGSVSVAGI DGGMASREVE NGGDDPLRLD GHGGIAVAPA AGGGLSSAAA HRARSNGAVK 

       190        200        210        220        230        240 
ALPKKSAFTP FYAIKKFKTE REGVEQLHYT GISQSACREM SLCRELDNKH LTKLVEIFLE 

       250        260        270        280        290        300 
RKSIYMVSEF AEHDLLQIIH FHSHPEKRLI APRMLKSIMW QILDGVSYLH QNWILHRDLK 

       310        320        330        340        350        360 
PANIMVTVDG CVKIGDLGLA RKFYNLVQTL YTGDKVVVTI WYRAPELLLG ARHYSPAIDL 

       370        380        390        400        410        420 
WAVGCIFAEL IGLRPIFKGE EAKMDSKKSV PFQGNQLQRI LEVLGTPTHH TWPNIHKYPE 

       430        440        450        460        470        480 
YEQLSKFSKY RDNLSVWYHS SGGRDKAALS LLYSLLKYDP ITRIDAIDAL EHEYFTNNDP 

       490        500        510        520        530        540 
PVSSDVFEGL SYKYPPRRIH TSDNDIMNVG ANKNKSGFNH HPPQQQTVNN NGVTNSSIGG 

       550        560        570        580 
LGVNRRILAA AAAAAAAVQV NGTNIVSGSS SSNGPIRKKK R

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]	Dietrich F.S., Voegeli S., Philippsen P. Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 368.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016820 Genomic DNA. Translation: AAS54242.2.
RefSeq	NP_986418.2. NM_211480.2.
3D structure databases
HSSP	HSSP built from PDB template 1V0B based on UniProtKB Q07785.
ProteinModelPortal	Q751F5.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_AGL249C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4622711.
KEGG	ago:AGOS_AGL249C.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
KO	K02208.
OMA	FHSHPEK.
OrthoDB	EOG470XRW.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SSN3_ASHGO

Accession

Primary (citable) accession number: Q751F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2013
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents