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Q751F5 (SSN3_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase SSN3

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cyclin-dependent kinase 8
Gene names
Name:SSN3
Synonyms:CDK8
Ordered Locus Names:AGL249C
OrganismAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]
Taxonomic identifier284811 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium

Protein attributes

Sequence length581 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactor

Magnesium By similarity.

Subunit structure

Component of the SRB8-11 complex, a regulatory module of the Mediator complex By similarity.

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 581581Serine/threonine-protein kinase SSN3
PRO_0000312932

Regions

Domain85 – 475391Protein kinase
Nucleotide binding91 – 999ATP By similarity
Compositional bias37 – 426Poly-Gln
Compositional bias549 – 5579Poly-Ala
Compositional bias567 – 5726Poly-Ser

Sites

Active site2981Proton acceptor By similarity
Binding site1951ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q751F5 [UniParc].

Last modified January 9, 2013. Version 2.
Checksum: 11B88D39F280B640

FASTA58164,530
        10         20         30         40         50         60 
MGIRLQSSDS MNNQHQTQQN KYFNVSNQAS TRSLWSQQQQ QQLLDTKGSA STSKSPMLMA 

        70         80         90        100        110        120 
NNNVFSIGPY RQRKDAGRVS VLEKYEIIGY IAAGTYGKVY KAKAKETQEE QENSIHTHNQ 

       130        140        150        160        170        180 
TLGSVSVAGI DGGMASREVE NGGDDPLRLD GHGGIAVAPA AGGGLSSAAA HRARSNGAVK 

       190        200        210        220        230        240 
ALPKKSAFTP FYAIKKFKTE REGVEQLHYT GISQSACREM SLCRELDNKH LTKLVEIFLE 

       250        260        270        280        290        300 
RKSIYMVSEF AEHDLLQIIH FHSHPEKRLI APRMLKSIMW QILDGVSYLH QNWILHRDLK 

       310        320        330        340        350        360 
PANIMVTVDG CVKIGDLGLA RKFYNLVQTL YTGDKVVVTI WYRAPELLLG ARHYSPAIDL 

       370        380        390        400        410        420 
WAVGCIFAEL IGLRPIFKGE EAKMDSKKSV PFQGNQLQRI LEVLGTPTHH TWPNIHKYPE 

       430        440        450        460        470        480 
YEQLSKFSKY RDNLSVWYHS SGGRDKAALS LLYSLLKYDP ITRIDAIDAL EHEYFTNNDP 

       490        500        510        520        530        540 
PVSSDVFEGL SYKYPPRRIH TSDNDIMNVG ANKNKSGFNH HPPQQQTVNN NGVTNSSIGG 

       550        560        570        580 
LGVNRRILAA AAAAAAAVQV NGTNIVSGSS SSNGPIRKKK R 

« Hide

References

« Hide 'large scale' references
[1]"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]"Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 368.
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016820 Genomic DNA. Translation: AAS54242.2.
RefSeqNP_986418.2. NM_211480.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING33169.AGOS_AGL249C.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4622711.
KEGGago:AGOS_AGL249C.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
KOK02208.
OrthoDBEOG7K3TWD.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSSN3_ASHGO
AccessionPrimary (citable) accession number: Q751F5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2013
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families