Q751E8 (PHO85_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Negative regulator of the PHO system
EC=2.7.11.22

Alternative name(s):
Serine/threonine-protein kinase PHO85

Gene names

Name:	PHO85
Ordered Locus Names:	AGL242C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium › Ashbya gossypii

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	When phosphate concentrations are high it phosphorylates the PHO4 transcription factor thus establishing repression By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with a number of cyclins By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Negative regulator of the PHO system		PRO_0000086515
Regions
	Domain	7 – 297	291	Protein kinase
	Nucleotide binding	13 – 21	9	ATP By similarity
Sites
	Active site	133	1	Proton acceptor By similarity
	Binding site	36	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q751E8 [UniParc]. Last modified January 9, 2013. Version 2. Checksum: 576D8B7FE50C8619	FASTA	301	34,366
	10 20 30 40 50 60 MTSTSQFKQL ERLGNGTYAT VYKGLNKTTG LYVALKEVKL DSEEGTPSTA IREISLMKEL 70 80 90 100 110 120 KHENIVRLYD VIHTENKLTL VFEFMDNDLK KFMDSRLDRE MPRGLELSLV KYFQWQLLQG 130 140 150 160 170 180 VAFCHENRIL HRDLKPQNLL INNKGQLKLG DFGLARAFGI PVNTFSSEVV TLWYRAPDVL 190 200 210 220 230 240 MGSRTYCTSI DIWSCGCILA EMIMGKALFP GTNDDEQLKL IFETMGTPTE QTWVGVSQLP 250 260 270 280 290 300 KYNPQIPLYP NKDIKQLLQA TTKEQISDVL VNLIQGLLQL NPSMRLSAQQ ALSHPLFEEY H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]	Dietrich F.S., Voegeli S., Philippsen P. Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016820 Genomic DNA. Translation: AAS54249.2.
RefSeq	NP_986425.2. NM_211487.2.
3D structure databases
ProteinModelPortal	Q751E8.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_AGL242C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4622718.
KEGG	ago:AGOS_AGL242C.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
KO	K06655.
OMA	KELKHES.
OrthoDB	EOG4QJVX0.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHO85_ASHGO

Accession

Primary (citable) accession number: Q751E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	January 9, 2013
Last modified:	May 1, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program