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Q750E0

- HEM2_ASHGO

UniProt

Q750E0 - HEM2_ASHGO

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Protein

Delta-aminolevulinic acid dehydratase

Gene

HEM2

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 1 zinc ion per monomer.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi134 – 1341Zinc; catalyticBy similarity
Metal bindingi136 – 1361Zinc; catalyticBy similarity
Metal bindingi144 – 1441Zinc; catalyticBy similarity
Active sitei211 – 2111Schiff-base intermediate with substrateBy similarity
Binding sitei221 – 2211Substrate 1By similarity
Binding sitei233 – 2331Substrate 1By similarity
Active sitei264 – 2641Schiff-base intermediate with substrateBy similarity
Binding sitei291 – 2911Substrate 2By similarity
Binding sitei330 – 3301Substrate 2By similarity

GO - Molecular functioni

  1. lead ion binding Source: UniProtKB
  2. porphobilinogen synthase activity Source: UniProtKB
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. heme biosynthetic process Source: UniProtKB
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:HEM2
Ordered Locus Names:AGR015C
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
ProteomesiUP000000591: Chromosome VII

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Delta-aminolevulinic acid dehydratasePRO_0000140531Add
BLAST

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi33169.AGOS_AGR015C.

Structurei

3D structure databases

ProteinModelPortaliQ750E0.
SMRiQ750E0. Positions 3-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

eggNOGiCOG0113.
HOGENOMiHOG000020323.
InParanoidiQ750E0.
KOiK01698.
OMAiSGYFHPT.
OrthoDBiEOG779P7W.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q750E0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKHTAEFLDT HQTQISSILS GGYNHPLLRE WQNERQLAKN MFIFPLFVSD
60 70 80 90 100
MPDEDQPIES LPNIRRFGVN KLAGYLKPLV AKGLRAVLLF GVPMKPNSKD
110 120 130 140 150
EFGSAADDPE GPVIQAIKLL RAEFPELYIL CDVCLCEYTS HGHCGVLYDD
160 170 180 190 200
GSINREQSVR RLAAVAVNYA KAGAHSVAPS DMIDGRIRDI KLGLLAAGLA
210 220 230 240 250
HKTFVMSYAA KFSGNLYGPF RDAACSAPSQ GDRKCYQLPS GGRGLARRAL
260 270 280 290 300
RRDLDEGADG IIVKPSTFYL DIMADASEIA KDVPICAYHV SGEYAMLHAA
310 320 330 340
AKAGVVDFKS IAFESHQGFL RAGARLIISY MTPEFLDWLS
Length:340
Mass (Da):37,331
Last modified:July 5, 2004 - v1
Checksum:iEA5FAB60A7562999
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016820 Genomic DNA. Translation: AAS54504.1.
RefSeqiNP_986680.1. NM_211742.1.

Genome annotation databases

EnsemblFungiiAAS54504; AAS54504; AGOS_AGR015C.
GeneIDi4622979.
KEGGiago:AGOS_AGR015C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016820 Genomic DNA. Translation: AAS54504.1 .
RefSeqi NP_986680.1. NM_211742.1.

3D structure databases

ProteinModelPortali Q750E0.
SMRi Q750E0. Positions 3-339.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 33169.AGOS_AGR015C.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii AAS54504 ; AAS54504 ; AGOS_AGR015C .
GeneIDi 4622979.
KEGGi ago:AGOS_AGR015C.

Phylogenomic databases

eggNOGi COG0113.
HOGENOMi HOG000020323.
InParanoidi Q750E0.
KOi K01698.
OMAi SGYFHPT.
OrthoDBi EOG779P7W.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
    Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
    Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
  2. "Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
    Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
    G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Entry informationi

Entry nameiHEM2_ASHGO
AccessioniPrimary (citable) accession number: Q750E0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3