Q74ZZ0 (ERG27_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-keto-steroid reductase
EC=1.1.1.270

Gene names

Name:	ERG27
Ordered Locus Names:	AGR068W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the reduction of the keto group on the C-3 of sterols By similarity.
Catalytic activity	4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NADP+ = 4-alpha-methyl-5-alpha-cholest-7-en-3-one + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-keto sterol reductase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	3-keto-steroid reductase		PRO_0000054589
Sites
	Active site	203	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q74ZZ0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 74795DFA134E4B2D	FASTA	348	39,684
	10 20 30 40 50 60 MLICHQCLSS RLTYISNLGL NIAYRLIEQF TDDSKLVIVV TSRTLPRVRE VVDLIKTYAE 70 80 90 100 110 120 KCGKSGAVDF DYLLVDFTDM VSVLGAAYEL EKRYDAIHYF YANAAQGVYS GIDWLGATKA 130 140 150 160 170 180 VLRDPLDAVT NPTYKIQRVG VKSRDGLGLV FQANVFGPYY LIRRIIPLLA KGKAKVVWLS 190 200 210 220 230 240 SLMSDVKYLS LEDVELLRTD SSYEGSKRLV DLLHLATYKE LKSLGIHQYV THPGIFTSHS 250 260 270 280 290 300 FYQYLNFFTY YGMLFLFYLA RWLGSPWHNI QGYKGANAPI YVATLANPTF EHQALKYGSA 310 320 330 340 TYRDGMEYIA KHEIDPTGMH DAYKYIRDLA EEWDIKLKDQ ITIGRSLS

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016820 Genomic DNA. Translation: AAS54557.1.
RefSeq	NP_986733.1. NM_211795.1.
3D structure databases
ProteinModelPortal	Q74ZZ0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q74ZZ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4623034.
GenomeReviews	Gene locus AGR068W in contig AE016820_GR.
KEGG	ago:AGOS_AGR068W.
NMPDR	fig|33169.1.peg.4378.
Organism-specific databases
AGD	AGR068W.
Phylogenomic databases
eggNOG	fuNOG05767.
HOGENOM	HBG398311.
OMA	FLFYLAR.
OrthoDB	EOG4FBN2N.
PhylomeDB	Q74ZZ0.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K09827.
ProtoNet	Search...

Entry information

Entry name

ERG27_ASHGO

Accession

Primary (citable) accession number: Q74ZZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program