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Protein

tRNA-splicing endonuclease subunit SEN2

Gene

SEN2

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei268By similarity1
Active sitei276By similarity1
Active sitei307By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit SEN2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease SEN2
Gene namesi
Name:SEN2
Ordered Locus Names:AGR073C
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
Proteomesi
  • UP000000591 Componenti: Chromosome VII

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094591 – 356tRNA-splicing endonuclease subunit SEN2Add BLAST356

Interactioni

Subunit structurei

Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN54 (By similarity).By similarity

Protein-protein interaction databases

STRINGi33169.AAS54562

Structurei

3D structure databases

SMRiQ74ZY5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

HOGENOMiHOG000159660
InParanoidiQ74ZY5
KOiK15322
OMAiHITGEYD
OrthoDBiEOG092C2YVE

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006676 tRNA_splic
IPR016589 tRNA_splic_SEN2
PANTHERiPTHR21227 PTHR21227, 1 hit
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PIRSFiPIRSF011789 tRNA_splic_SEN2, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

Q74ZY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRVAGSKR YVHPLPIEPV VLPPLIAHNP LSWVYWVLAY VTSSNQLPRK
60 70 80 90 100
IPLEVGADGR YTVTGREQMQ YLWEHGFFGT GQLSRSEPTW QARTVDRLQL
110 120 130 140 150
DTEGIAGHKL EQVTQLRRKQ RLEFKRERAS FERKRLELRR QGVLESEILE
160 170 180 190 200
QERLWLKQLR DRELQWEAST GDPSPVRAED AEIIAEDGAS VLPIEKLELM
210 220 230 240 250
PVEALFLTLA LPVLHADAPA ILARTLGPQP ALPQIERLCR LYAAYHHYRS
260 270 280 290 300
HGWCVRSGIK FGCDFLLYRR GPPFHHAEFS VMVLAPDERH DYTWYSTVAR
310 320 330 340 350
VVGGAQKTLV LAYVARRAAA DQLAALWHAR RYMEAFALFE VHELVYRRWL

PGKNRE
Length:356
Mass (Da):41,096
Last modified:January 9, 2013 - v2
Checksum:i37FD39B17F5D8D5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016820 Genomic DNA Translation: AAS54562.2
RefSeqiNP_986738.2, NM_211800.2

Genome annotation databases

EnsemblFungiiAAS54562; AAS54562; AGOS_AGR073C
GeneIDi4623039
KEGGiago:AGOS_AGR073C

Similar proteinsi

Entry informationi

Entry nameiSEN2_ASHGO
AccessioniPrimary (citable) accession number: Q74ZY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 9, 2013
Last modified: April 25, 2018
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health