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Q74ZY5

- SEN2_ASHGO

UniProt

Q74ZY5 - SEN2_ASHGO

Protein

tRNA-splicing endonuclease subunit SEN2

Gene

SEN2

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 2 (09 Jan 2013)
      Previous versions | rss
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    Functioni

    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei268 – 2681By similarity
    Active sitei276 – 2761By similarity
    Active sitei307 – 3071By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: InterPro

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonuclease subunit SEN2 (EC:4.6.1.16)
    Alternative name(s):
    tRNA-intron endonuclease SEN2
    Gene namesi
    Name:SEN2
    Ordered Locus Names:AGR073C
    OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
    Taxonomic identifieri284811 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
    ProteomesiUP000000591: Chromosome VII

    Subcellular locationi

    GO - Cellular componenti

    1. tRNA-intron endonuclease complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356tRNA-splicing endonuclease subunit SEN2PRO_0000109459Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN54 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi33169.AGOS_AGR073C.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family.Curated

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000159660.
    KOiK15322.
    OrthoDBiEOG7SXWFH.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR016589. tRNA_splic_SEN2.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    [Graphical view]
    PIRSFiPIRSF011789. tRNA_splic_SEN2. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q74ZY5-1 [UniParc]FASTAAdd to Basket

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    MAKRVAGSKR YVHPLPIEPV VLPPLIAHNP LSWVYWVLAY VTSSNQLPRK    50
    IPLEVGADGR YTVTGREQMQ YLWEHGFFGT GQLSRSEPTW QARTVDRLQL 100
    DTEGIAGHKL EQVTQLRRKQ RLEFKRERAS FERKRLELRR QGVLESEILE 150
    QERLWLKQLR DRELQWEAST GDPSPVRAED AEIIAEDGAS VLPIEKLELM 200
    PVEALFLTLA LPVLHADAPA ILARTLGPQP ALPQIERLCR LYAAYHHYRS 250
    HGWCVRSGIK FGCDFLLYRR GPPFHHAEFS VMVLAPDERH DYTWYSTVAR 300
    VVGGAQKTLV LAYVARRAAA DQLAALWHAR RYMEAFALFE VHELVYRRWL 350
    PGKNRE 356
    Length:356
    Mass (Da):41,096
    Last modified:January 9, 2013 - v2
    Checksum:i37FD39B17F5D8D5C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016820 Genomic DNA. Translation: AAS54562.2.
    RefSeqiNP_986738.2. NM_211800.2.

    Genome annotation databases

    GeneIDi4623039.
    KEGGiago:AGOS_AGR073C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016820 Genomic DNA. Translation: AAS54562.2 .
    RefSeqi NP_986738.2. NM_211800.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 33169.AGOS_AGR073C.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4623039.
    KEGGi ago:AGOS_AGR073C.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000159660.
    KOi K15322.
    OrthoDBi EOG7SXWFH.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR016589. tRNA_splic_SEN2.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF011789. tRNA_splic_SEN2. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
      Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
      Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
    2. "Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
      Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
      G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 299; 303; 306; 315; 319-320; 324 AND 339-340.
      Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

    Entry informationi

    Entry nameiSEN2_ASHGO
    AccessioniPrimary (citable) accession number: Q74ZY5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: January 9, 2013
    Last modified: October 1, 2014
    This is version 73 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3