Q74ZY5 (SEN2_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease subunit SEN2
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease SEN2

Gene names

Name:	SEN2
Ordered Locus Names:	AGR073C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium › Ashbya gossypii

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage By similarity.
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron.
Subunit structure	Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN54 By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: InterPro
Cellular_component	tRNA-intron endonuclease complex Inferred from electronic annotation. Source: InterPro
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	tRNA-splicing endonuclease subunit SEN2		PRO_0000109459
Sites
	Active site	268	1	By similarity
	Active site	276	1	By similarity
	Active site	307	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q74ZY5 [UniParc]. Last modified January 9, 2013. Version 2. Checksum: 37FD39B17F5D8D5C	FASTA	356	41,096
	10 20 30 40 50 60 MAKRVAGSKR YVHPLPIEPV VLPPLIAHNP LSWVYWVLAY VTSSNQLPRK IPLEVGADGR 70 80 90 100 110 120 YTVTGREQMQ YLWEHGFFGT GQLSRSEPTW QARTVDRLQL DTEGIAGHKL EQVTQLRRKQ 130 140 150 160 170 180 RLEFKRERAS FERKRLELRR QGVLESEILE QERLWLKQLR DRELQWEAST GDPSPVRAED 190 200 210 220 230 240 AEIIAEDGAS VLPIEKLELM PVEALFLTLA LPVLHADAPA ILARTLGPQP ALPQIERLCR 250 260 270 280 290 300 LYAAYHHYRS HGWCVRSGIK FGCDFLLYRR GPPFHHAEFS VMVLAPDERH DYTWYSTVAR 310 320 330 340 350 VVGGAQKTLV LAYVARRAAA DQLAALWHAR RYMEAFALFE VHELVYRRWL PGKNRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]	Dietrich F.S., Voegeli S., Philippsen P. Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 299; 303; 306; 315; 319-320; 324 AND 339-340.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016820 Genomic DNA. Translation: AAS54562.2.
RefSeq	NP_986738.2. NM_211800.2.
3D structure databases
ProteinModelPortal	Q74ZY5.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_AGR073C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4623039.
KEGG	ago:AGOS_AGR073C.
Phylogenomic databases
eggNOG	COG1676.
HOGENOM	HOG000159660.
KO	K15322.
OMA	CVRSGIK.
OrthoDB	EOG4PCF2K.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006676. tRNA_splic. IPR016589. tRNA_splic_SEN2. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. [Graphical view]
PIRSF	PIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit.
TIGRFAMs	TIGR00324. endA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SEN2_ASHGO

Accession

Primary (citable) accession number: Q74ZY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	January 9, 2013
Last modified:	May 1, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program