Q74ZW4 (ARGI_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginase
EC=3.5.3.1

Gene names

Name:	CAR1
Ordered Locus Names:	AGR225C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Arginine metabolism Urea cycle
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW urea cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arginase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Arginase		PRO_0000173706
Regions
	Region	218 – 222	5	Substrate binding By similarity
	Region	229 – 231	3	Substrate binding By similarity
Sites
	Metal binding	193	1	Manganese 1 By similarity
	Metal binding	216	1	Manganese 1 By similarity
	Metal binding	216	1	Manganese 2 By similarity
	Metal binding	218	1	Manganese 2 By similarity
	Metal binding	220	1	Manganese 1 By similarity
	Metal binding	322	1	Manganese 1 By similarity
	Metal binding	322	1	Manganese 2 By similarity
	Metal binding	324	1	Manganese 2 By similarity
	Binding site	273	1	Substrate By similarity
	Binding site	367	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q74ZW4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8DDCF23F497F51EB	FASTA	399	42,555
	10 20 30 40 50 60 MAQRNCWRRS WLGVGGGLCL GAHGAARGPR QLFHLTAKQS SAASDAKCRR WRAAGRGGGA 70 80 90 100 110 120 GGVYNGRGAA AGSRQTSGMA HNEHPHYKFF ESKKASLVMA PFSGGQGKSG VEDGPKYLLK 130 140 150 160 170 180 QGLREGVEGL GWEIEVQRPL DGHDFEERKQ RATEVVGRAK NPTLVGEATH LIHDAVRAAA 190 200 210 220 230 240 HAGRLPVTLG GDHSIAIGTV SGVLDRYPDA GLLWVDAHAD INTLASTESG NLHGCPVSFL 250 260 270 280 290 300 MGLERESWPA PLAWVPGTLR PSKIAYIGLR DVDPEEKEIL RRLGITAFSM YHVDRYGINK 310 320 330 340 350 360 VVEMALAAIN PDGTGPVMVS YDVDAIDPMY VPATGTPVRG GLTLREGLFI VERVAETGNL 370 380 390 VALDVVECNP ELAAHDLHVV DTVQTGCSIA RCALGETLL

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016820 Genomic DNA. Translation: AAS54715.1.
RefSeq	NP_986891.1. NM_211953.1.
3D structure databases
ProteinModelPortal	Q74ZW4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q74ZW4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4623193.
GenomeReviews	Gene locus AGR225C in contig AE016820_GR.
KEGG	ago:AGOS_AGR225C.
NMPDR	fig|33169.1.peg.4536.
Organism-specific databases
AGD	AGR225C.
Phylogenomic databases
eggNOG	fuNOG04820.
HOGENOM	HBG391953.
OMA	DRHGIGK.
OrthoDB	EOG432471.
PhylomeDB	Q74ZW4.
Family and domain databases
InterPro	IPR014033. Arginase_subgr. IPR006035. Ureohydrolase. IPR023696. Ureohydrolase_domain. IPR020855. Ureohydrolase_Mn_BS. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
KO	K01476.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PIRSF	PIRSF036979. Arginase. 1 hit.
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. RocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGI_ASHGO

Accession

Primary (citable) accession number: Q74ZW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program