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Q74ZR2 (PP4C_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit

Short name=PP4C
EC=3.1.3.16
Gene names
Name:PPH3
Ordered Locus Names:AGR136W
OrganismAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]
Taxonomic identifier284811 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the dephosphorylation and activation of the transcription factor GLN3 in response to nutrient availability. Forms the histone H2A phosphatase complex in association with the regulatory subunits PSY2 and PSY4, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint By similarity.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Subunit structure

Catalytic subunit of the histone H2A phosphatase complex (HTP-C) containing PPH3, PSY2 and PSY4 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   LigandManganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   PTMMethylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoprotein phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 310310Serine/threonine-protein phosphatase 4 catalytic subunit
PRO_0000223649

Sites

Active site1141Proton donor By similarity
Metal binding531Manganese 1 By similarity
Metal binding551Manganese 1 By similarity
Metal binding811Manganese 1 By similarity
Metal binding811Manganese 2 By similarity
Metal binding1131Manganese 2 By similarity
Metal binding1631Manganese 2 By similarity
Metal binding2371Manganese 2 By similarity

Amino acid modifications

Modified residue3101Leucine methyl ester By similarity

Sequences

Sequence LengthMass (Da)Tools
Q74ZR2 [UniParc].

Last modified October 11, 2004. Version 2.
Checksum: 1B54B7CC11AF3975

FASTA31035,699
        10         20         30         40         50         60 
MAGMQLDEII EYLRYSKHIP EETIYELCLK CQELLVNESN VTHVDTPVTI CGDIHGQLHD 

        70         80         90        100        110        120 
LLTLFEKSEG IEKNRFIFLG DFVDRGFYSL ESFLLLLCYK LRYPDRITLI RGNHETRQIT 

       130        140        150        160        170        180 
KVYGFYDEVI RKYGNSNVWR YCCEVFDYLS LGAIINGQIF CVHGGLSPDV MTVDEIRSID 

       190        200        210        220        230        240 
RKQEVPHEGA MCDLLWSDPD EVDTWSLSPR GAGFLFGKNE VDQFLHRNDI SLIARAHQLV 

       250        260        270        280        290        300 
MEGYKEMFDG GLVTVWSAPN YCYRCGNVAA VLRIDDDLSR NYTIFEAVPA QDNRGNAIIP 

       310 
TKKPQMDYFL 

« Hide

References

« Hide 'large scale' references
[1]"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]"Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016820 Genomic DNA. Translation: AAS54626.2.
RefSeqNP_986802.2. NM_211864.2.

3D structure databases

ProteinModelPortalQ74ZR2.
SMRQ74ZR2. Positions 5-309.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING33169.AGOS_AGR136W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiAAS54626; AAS54626; AGOS_AGR136W.
GeneID4623104.
KEGGago:AGOS_AGR136W.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172696.
KOK15423.
OMANDISMIC.
OrthoDBEOG7FFN29.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP4C_ASHGO
AccessionPrimary (citable) accession number: Q74ZR2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: October 11, 2004
Last modified: July 9, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families