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Q74ZK8

- TRMB_ASHGO

UniProt

Q74ZK8 - TRMB_ASHGO

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei177 – 1771S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei180 – 1801UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
Ordered Locus Names:AGR192C
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
ProteomesiUP000000591: Chromosome VII

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282tRNA (guanine-N(7)-)-methyltransferasePRO_0000370583Add
BLAST

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Protein-protein interaction databases

STRINGi33169.AGOS_AGR192C.

Structurei

3D structure databases

ProteinModelPortaliQ74ZK8.
SMRiQ74ZK8. Positions 56-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 1232S-adenosyl-L-methionine bindingUniRule annotation
Regioni157 – 1582S-adenosyl-L-methionine bindingUniRule annotation
Regioni255 – 2573S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000260965.
InParanoidiQ74ZK8.
KOiK03439.
OMAiRAHSNPI.
OrthoDBiEOG708W9T.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q74ZK8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLTDDQASK RQAYRAAKEA NRKELKHVKI DDALPNKSSE LDLPKKKFYR
60 70 80 90 100
QRAHSNPFSD HQLEYPLSPA EMDWSKLYPH YYDAATNTMT KKVTVADIGC
110 120 130 140 150
GYGGLMVELS RELPENLILG MEIRVQVTNY VEDRIIALRT NHAAQKEYQN
160 170 180 190 200
INVLRGNAMK FLPNFFEKSQ LEKMFFCFPD PHFKQRKHKA RIITNTLLSE
210 220 230 240 250
YAYVLKEGGV IYTITDVLDL HNWMVEHLNM HPLFDRLGDE WLDQDPCVRI
260 270 280
MRHATEEGKK VARKQGDKFV ACFRRLPNPE LV
Length:282
Mass (Da):32,889
Last modified:July 5, 2004 - v1
Checksum:i6935EABEDB0BD2FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016820 Genomic DNA. Translation: AAS54682.1.
RefSeqiNP_986858.1. NM_211920.1.

Genome annotation databases

EnsemblFungiiAAS54682; AAS54682; AGOS_AGR192C.
GeneIDi4623160.
KEGGiago:AGOS_AGR192C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016820 Genomic DNA. Translation: AAS54682.1 .
RefSeqi NP_986858.1. NM_211920.1.

3D structure databases

ProteinModelPortali Q74ZK8.
SMRi Q74ZK8. Positions 56-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 33169.AGOS_AGR192C.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii AAS54682 ; AAS54682 ; AGOS_AGR192C .
GeneIDi 4623160.
KEGGi ago:AGOS_AGR192C.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000260965.
InParanoidi Q74ZK8.
KOi K03439.
OMAi RAHSNPI.
OrthoDBi EOG708W9T.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
    Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
    Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
  2. "Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
    Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
    G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Entry informationi

Entry nameiTRMB_ASHGO
AccessioniPrimary (citable) accession number: Q74ZK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3