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Q74ZK4 (GSHR_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione reductase

Short name=GR
Short name=GRase
EC=1.8.1.7
Gene names
Name:GLR1
Ordered Locus Names:AGR196W
OrganismAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]
Taxonomic identifier284811 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium

Protein attributes

Sequence length480 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Maintains high levels of reduced glutathione in the cytosol By similarity.

Catalytic activity

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactor

Binds 1 FAD per subunit By similarity.

Subcellular location

Cytoplasm By similarity.

Miscellaneous

The active site is a redox-active disulfide bond By similarity.

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 480480Glutathione reductase
PRO_0000067965

Regions

Nucleotide binding51 – 599FAD By similarity

Sites

Active site4691Proton acceptor By similarity

Amino acid modifications

Disulfide bond59 ↔ 64Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
Q74ZK4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 8AF0C57EEA727BC9

FASTA48052,649
        10         20         30         40         50         60 
MLRCREVYNI CKGAMSNEVK HYDYLVIGGG SGGVASSRRA ASYGAKTVLI EGKALGGTCV 

        70         80         90        100        110        120 
NVGCVPKKVM WYASDLAHRL LHARDYGLLQ EVDISKEKLH FNWKEFAGKR NAYVERLNGI 

       130        140        150        160        170        180 
YERNLAKEGV EYVHGWARFN SEGQVEVTRP DQTTEKYTAD HILIATGGEP VLPEGIPGAE 

       190        200        210        220        230        240 
YGVDSDGFFR LEEQPKKVVI SGSGYIATEF AGVFNGLGTE THIVIRKDHV LTKFDPSIQE 

       250        260        270        280        290        300 
IVTEHYEKEG VNIHKKESIQ RVEKDPNTGK LTVHLSGKII EDVDQLVWAI GRKSLLGIAP 

       310        320        330        340        350        360 
ENVGVKLGET GHVVVDEYQN TSTKGIYALG DVVGNMELTP VAIAAGRKLA NRLFGPEQMR 

       370        380        390        400        410        420 
AQKQDYDNVP SVVFSHPEAG SIGLTEPQAI ERYGKENIKI YQTKFTAMYY AMLEDKSPTK 

       430        440        450        460        470        480 
YKLICAGPEE KVVGLHIVGD GSAEILQGFG VAIKMGATKA DFDSCVAIHP TSAEEIVTLK 

« Hide

References

« Hide 'large scale' references
[1]"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]"Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016820 Genomic DNA. Translation: AAS54686.1.
RefSeqNP_986862.1. NM_211924.1.

3D structure databases

ProteinModelPortalQ74ZK4.
SMRQ74ZK4. Positions 20-480.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING33169.AGOS_AGR196W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiAAS54686; AAS54686; AGOS_AGR196W.
GeneID4623164.
KEGGago:AGOS_AGR196W.

Phylogenomic databases

eggNOGCOG1249.
HOGENOMHOG000276712.
KOK00383.
OMAHRQPCKM.
OrthoDBEOG79W9F2.
PhylomeDBQ74ZK4.

Family and domain databases

Gene3D3.30.390.30. 1 hit.
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006322. Glutathione_Rdtase_euk/bac.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. SSF55424. 1 hit.
TIGRFAMsTIGR01421. gluta_reduc_1. 1 hit.
PROSITEPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHR_ASHGO
AccessionPrimary (citable) accession number: Q74ZK4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: December 11, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families