Reviewed, UniProtKB/Swiss-Prot Q74ZK4 (GSHR_ASHGO)

Names and origin

Protein names

Recommended name:
    Glutathione reductase
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:	GLR1
Ordered Locus Names:	AGR196W

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol By similarity.
Catalytic activity	2 glutathione + NADP+ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond By similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Glutathione reductase		PRO_0000067965
Regions
	Nucleotide binding	51 – 59	9	FAD By similarity
Sites
	Active site	469	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	59 ↔ 64		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q74ZK4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8AF0C57EEA727BC9	FASTA	480	52,649
	10 20 30 40 50 60 MLRCREVYNI CKGAMSNEVK HYDYLVIGGG SGGVASSRRA ASYGAKTVLI EGKALGGTCV 70 80 90 100 110 120 NVGCVPKKVM WYASDLAHRL LHARDYGLLQ EVDISKEKLH FNWKEFAGKR NAYVERLNGI 130 140 150 160 170 180 YERNLAKEGV EYVHGWARFN SEGQVEVTRP DQTTEKYTAD HILIATGGEP VLPEGIPGAE 190 200 210 220 230 240 YGVDSDGFFR LEEQPKKVVI SGSGYIATEF AGVFNGLGTE THIVIRKDHV LTKFDPSIQE 250 260 270 280 290 300 IVTEHYEKEG VNIHKKESIQ RVEKDPNTGK LTVHLSGKII EDVDQLVWAI GRKSLLGIAP 310 320 330 340 350 360 ENVGVKLGET GHVVVDEYQN TSTKGIYALG DVVGNMELTP VAIAAGRKLA NRLFGPEQMR 370 380 390 400 410 420 AQKQDYDNVP SVVFSHPEAG SIGLTEPQAI ERYGKENIKI YQTKFTAMYY AMLEDKSPTK 430 440 450 460 470 480 YKLICAGPEE KVVGLHIVGD GSAEILQGFG VAIKMGATKA DFDSCVAIHP TSAEEIVTLK

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016820 Genomic DNA. Translation: AAS54686.1.
RefSeq	NP_986862.1.
3D structure databases
SMR	Q74ZK4. Positions 20-480.
ModBase	Search...
Protein-protein interaction databases
STRING	Q74ZK4.
Genome annotation databases
GeneID	4623164.
GenomeReviews	Gene locus AGR196W in contig AE016820_GR.
KEGG	ago:AGOS_AGR196W.
NMPDR	fig|33169.1.peg.4507.
Organism-specific databases
AGD	AGR196W.
Phylogenomic databases
eggNOG	fuNOG04327.
HOGENOM	HBG515043.
OMA	WYASDLA.
OrthoDB	EOG9M3BRT.
Enzyme and pathway databases
BRENDA	1.8.1.7. 279361.
Family and domain databases
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006322. Glutathione_Rdtase_animal/bac. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
TIGRFAMs	TIGR01421. gluta_reduc_1. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHR_ASHGO

Accession

Primary (citable) accession number: Q74ZK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)