Q74ZF0 (DUT_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	DUT1
Ordered Locus Names:	AGR249C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium › Ashbya gossypii

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity.
Catalytic activity	dUTP + H2O = dUMP + diphosphate.
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
Subunit structure	Homotrimer By similarity.
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dITP catabolic process Inferred from electronic annotation. Source: EnsemblFungi dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP catabolic process Inferred from electronic annotation. Source: EnsemblFungi
Cellular_component	cytoplasm Inferred from electronic annotation. Source: EnsemblFungi nucleus Inferred from electronic annotation. Source: EnsemblFungi
Molecular_function	dITP diphosphatase activity Inferred from electronic annotation. Source: EnsemblFungi dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 153	153	Deoxyuridine 5'-triphosphate nucleotidohydrolase		PRO_0000182929
Regions
	Region	74 – 76	3	Substrate binding By similarity
	Region	88 – 91	4	Substrate binding By similarity
	Region	148 – 149	2	Substrate binding By similarity
Sites
	Binding site	99	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	143	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q74ZF0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B642014781B1B4E2	FASTA	153	16,071
	10 20 30 40 50 60 MTDQPAKKVH SAPTLKVQLR SENAIAPTKG SAAAAGYDIY ASQDCVIPGR GQGLVATDVS 70 80 90 100 110 120 FTVPVGTYGR IAPRSGLAVK HGIQTGAGVV DRDYTGEVKI VLFNHSDRDY AVKRGDRVAQ 130 140 150 LVLERIVDDA EVVVVESLDE SSRGEGGFGS TGN

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016820 Genomic DNA. Translation: AAS54739.1.
RefSeq	NP_986915.1. NM_211977.1.
3D structure databases
ProteinModelPortal	Q74ZF0.
SMR	Q74ZF0. Positions 12-140.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_AGR249C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	AAS54739; AAS54739; AGOS_AGR249C.
GeneID	4623217.
KEGG	ago:AGOS_AGR249C.
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HOG000028966.
KO	K01520.
OMA	KVCLINH.
OrthoDB	EOG47M57F.
PhylomeDB	Q74ZF0.
Enzyme and pathway databases
UniPathway	UPA00610; UER00666.
Family and domain databases
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_ASHGO

Accession

Primary (citable) accession number: Q74ZF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program