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Protein

Protein N-terminal and lysine N-methyltransferase EFM7

Gene

EFM7

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent protein methyltransferase that trimethylates the N-terminal glycine 'Gly-2' of elongation factor 1-alpha, before also catalyzing the mono- and dimethylation of 'Lys-3'.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei67S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei115S-adenosyl-L-methionineUniRule annotation1
Binding sitei152S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei176S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-terminal and lysine N-methyltransferase EFM7UniRule annotation (EC:2.1.1.-UniRule annotation)
Alternative name(s):
Elongation factor methyltransferase 7UniRule annotation
Gene namesi
Name:EFM7UniRule annotation
Synonyms:NNT1
Ordered Locus Names:AGR284W
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
Proteomesi
  • UP000000591 Componenti: Chromosome VII

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968891 – 265Protein N-terminal and lysine N-methyltransferase EFM7Add BLAST265

Structurei

3D structure databases

ProteinModelPortaliQ74ZB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 95S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM7 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000199189.
InParanoidiQ74ZB5.
KOiK17878.
OMAiVVFSPHR.
OrthoDBiEOG092C4ME2.

Family and domain databases

HAMAPiMF_03223. Methyltr_EFM7. 1 hit.
InterProiView protein in InterPro
IPR019410. Methyltransf_16.
IPR025784. Nicotinamide_N-MeTfrase_put.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF10294. Methyltransf_16. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiView protein in PROSITE
PS51560. SAM_MT_NNT1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q74ZB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDHEEDSL YGATELFGEP DGFYEKPAES HFAEYERSAV PAQSARRDTQ
60 70 80 90 100
VRIRLVGSSP LWGHLLWNSA IYTARHLDAH PEQVVGRCVL ELGAAGALPS
110 120 130 140 150
LVAGLLGARQ VVATDYPDAD LVGNIQYNVD HVIYGGKPPT EAPHVAVEGY
160 170 180 190 200
IWGNDYGPLR RHLPPGQTGF DLVLLSDLVF NHTEHHKLLQ TTRDLLAPAG
210 220 230 240 250
RALVVFSPHR PWLLEKDLQF FETAAEYGLR AELIEQVTWA PMFADDPGPA
260
EVRARVYAYY LTHCA
Length:265
Mass (Da):29,379
Last modified:July 5, 2004 - v1
Checksum:i606D6F1F87AFED1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016820 Genomic DNA. Translation: AAS54774.1.
RefSeqiNP_986950.1. NM_212012.1.

Genome annotation databases

EnsemblFungiiAAS54774; AAS54774; AGOS_AGR284W.
GeneIDi4623252.
KEGGiago:AGOS_AGR284W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016820 Genomic DNA. Translation: AAS54774.1.
RefSeqiNP_986950.1. NM_212012.1.

3D structure databases

ProteinModelPortaliQ74ZB5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAAS54774; AAS54774; AGOS_AGR284W.
GeneIDi4623252.
KEGGiago:AGOS_AGR284W.

Phylogenomic databases

HOGENOMiHOG000199189.
InParanoidiQ74ZB5.
KOiK17878.
OMAiVVFSPHR.
OrthoDBiEOG092C4ME2.

Family and domain databases

HAMAPiMF_03223. Methyltr_EFM7. 1 hit.
InterProiView protein in InterPro
IPR019410. Methyltransf_16.
IPR025784. Nicotinamide_N-MeTfrase_put.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF10294. Methyltransf_16. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiView protein in PROSITE
PS51560. SAM_MT_NNT1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEFM7_ASHGO
AccessioniPrimary (citable) accession number: Q74ZB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: July 5, 2004
Last modified: May 10, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.