Q74Z75 (BUD32_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase BUD32
EC=2.7.11.1

Gene names

Name:	BUD32
Ordered Locus Names:	AGR331C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the EKC/KEOPS complex which promotes both telomere uncapping and telomere elongation By similarity. The complex is required for efficient recruitment of transcriptional coactivators. Important for bud site selection By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Component of the EKC/KEOPS complex By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Chromosome › telomere By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Chromosome Cytoplasm Nucleus Telomere
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Serine/threonine-protein kinase BUD32		PRO_0000278904
Regions
	Domain	16 – 260	245	Protein kinase
	Nucleotide binding	22 – 30	9	ATP By similarity
Sites
	Active site	160	1	Proton acceptor By similarity
	Binding site	51	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q74Z75 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8BC8D39DAE4CA046	FASTA	260	29,235
	10 20 30 40 50 60 MSAEVISRVS QIYGEGITLT PISQGAEAVI FTTTTHPYLP STEGSSKYVV KYRPPKTYRH 70 80 90 100 110 120 PSIDVALTKQ RTLGEARLLG KLQQIKGLRV PKLLACDVYN GSIWMEFLGE DLPESRGFSN 130 140 150 160 170 180 LKNFLWMCAS EPYSPVVEAT VRDVGCQIGL LHWNDYCHGD LTTSNIVLVR SGAEWAPHLI 190 200 210 220 230 240 DFGLGSNSNL VEDKGVDLYV LERALASTHS AFAEKYFEWL MEGFTSVYQS HGEKGEQKLK 250 260 ELLRRFEEVR LRGRKRSMLG

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016820 Genomic DNA. Translation: AAS54821.1.
RefSeq	NP_986997.1. NM_212059.1.
3D structure databases
ProteinModelPortal	Q74Z75.
ModBase	Search...
Protein-protein interaction databases
STRING	Q74Z75.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4623300.
GenomeReviews	Gene locus AGR331C in contig AE016820_GR.
KEGG	ago:AGOS_AGR331C.
NMPDR	fig|33169.1.peg.4642.
Organism-specific databases
AGD	AGR331C.
Phylogenomic databases
eggNOG	fuNOG08130.
HOGENOM	HBG574115.
OMA	IGMERIE.
OrthoDB	EOG4R7ZKZ.
PhylomeDB	Q74Z75.
Family and domain databases
InterPro	IPR022495. Kinase-assoc_endopept-1. IPR011009. Kinase-like_dom. IPR010440. LipoPS_kinase. IPR000719. Prot_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
KO	K08851.
Pfam	PF06293. Kdo. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
TIGRFAMs	TIGR03724. Arch_bud32. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUD32_ASHGO

Accession

Primary (citable) accession number: Q74Z75

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program