Q74Z34 (UBC6_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 6
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein UBC6
Ubiquitin-protein ligase UBC6

Gene names

Name:	UBC6
Ordered Locus Names:	AGR372W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium › Ashbya gossypii

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Endoplasmic reticulum membrane By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Ubiquitin-conjugating enzyme E2 6		PRO_0000082547
Regions
	Topological domain	1 – 217	217	Cytoplasmic Potential
	Transmembrane	218 – 240	23	Helical; Potential
Sites
	Active site	87	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q74Z34 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5F3FC501A5BC21DB	FASTA	242	27,612
	10 20 30 40 50 60 MASRQAYKRL SKEYKMMTEN PPPYIVAAPK EDNILVWHYV ITGPPETPYE DGQYHGTLVF 70 80 90 100 110 120 PNDYPFNPPA IRMLTPNGRF RENTRLCLSM SDYHPDTWNP SWSVATILTG LLSFMTTDES 130 140 150 160 170 180 SIGTMESTDS TKRKYAAKSK EHNATRSPIF CQIFPELAEQ NRRDIEEAKK RKDETVVEDE 190 200 210 220 230 240 HPFKQEQVVS LEEINDPEDR IRAQMLQQPA GNGTTSNSIG RSLLFVLFSL AALLVAVCYT RV

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016820 Genomic DNA. Translation: AAS54862.1.
RefSeq	NP_987038.1. NM_212100.1.
3D structure databases
ProteinModelPortal	Q74Z34.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_AGR372W.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	AAS54862; AAS54862; AGOS_AGR372W.
GeneID	4623341.
KEGG	ago:AGOS_AGR372W.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000170329.
KO	K04554.
OMA	CKTPAFR.
OrthoDB	EOG441TMP.
PhylomeDB	Q74Z34.
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC6_ASHGO

Accession

Primary (citable) accession number: Q74Z34

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program