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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei82NucleophileUniRule annotation1
Active sitei207UniRule annotation1
Active sitei235UniRule annotation1
Binding sitei235SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00078.

Protein family/group databases

ESTHERiyerpe-BIOH. BioH.
MEROPSiS33.994.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:YPO0129, y3908, YP_0130
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002045061 – 258Pimeloyl-[acyl-carrier protein] methyl ester esteraseAdd BLAST258

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y3908.

Structurei

3D structure databases

ProteinModelPortaliQ74Y45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 242AB hydrolase-1Sequence analysisAdd BLAST227

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83Substrate bindingUniRule annotation2
Regioni143 – 147Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3C. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028062.
KOiK02170.
OMAiLQDFADD.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q74Y45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQLYWYTCG EGDCDLVLLH GWGLNSGVWH CIIDRLAPHF RLHLVDLPGY
60 70 80 90 100
GRSQDYGAMS LADMAERVAQ QAPKQALWLG WSMGGLVASQ IALSQPECVR
110 120 130 140 150
GLITVSSSPC FTARDEWPGI KPEVLAGFQH QLSDDFHRTV ERFLALQTLG
160 170 180 190 200
TESSRQDARL LKSVVLQHQM PDVEVLTGGL AILRTADLRT ALAGFTLPFM
210 220 230 240 250
RVYGHLDSLV PRKVASLLDS AWPQTQSVVM QGAAHAPFIS HPNDFAKLIL

NFAEENKK
Length:258
Mass (Da):28,590
Last modified:August 16, 2005 - v2
Checksum:i5866E222A2C6D145
GO

Sequence cautioni

The sequence AAM87452 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS60408 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL18815.1.
AE009952 Genomic DNA. Translation: AAM87452.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS60408.1. Different initiation.
PIRiAF0016.
RefSeqiWP_002208922.1. NZ_LQBA01000032.1.
YP_002345215.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM87452; AAM87452; y3908.
AAS60408; AAS60408; YP_0130.
GeneIDi1172975.
KEGGiype:YPO0129.
ypk:y3908.
ypm:YP_0130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL18815.1.
AE009952 Genomic DNA. Translation: AAM87452.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS60408.1. Different initiation.
PIRiAF0016.
RefSeqiWP_002208922.1. NZ_LQBA01000032.1.
YP_002345215.1. NC_003143.1.

3D structure databases

ProteinModelPortaliQ74Y45.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187410.y3908.

Protein family/group databases

ESTHERiyerpe-BIOH. BioH.
MEROPSiS33.994.

Protocols and materials databases

DNASUi1148855.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM87452; AAM87452; y3908.
AAS60408; AAS60408; YP_0130.
GeneIDi1172975.
KEGGiype:YPO0129.
ypk:y3908.
ypm:YP_0130.

Phylogenomic databases

eggNOGiENOG4105D3C. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028062.
KOiK02170.
OMAiLQDFADD.

Enzyme and pathway databases

UniPathwayiUPA00078.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBIOH_YERPE
AccessioniPrimary (citable) accession number: Q74Y45
Secondary accession number(s): Q0WKH3, Q8CZK1, Q8ZJH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.