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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Yersinia pestis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei82NucleophileUniRule annotation1
Active sitei207UniRule annotation1
Active sitei235UniRule annotation1
Binding sitei235SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase
Biological processBiotin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00078

Protein family/group databases

ESTHERiyerpe-BIOH BioH

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:YPO0129, y3908, YP_0130
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002045061 – 258Pimeloyl-[acyl-carrier protein] methyl ester esteraseAdd BLAST258

Proteomic databases

PaxDbiQ74Y45

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y3908

Structurei

3D structure databases

ProteinModelPortaliQ74Y45
SMRiQ74Y45
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 242AB hydrolase-1Sequence analysisAdd BLAST227

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83Substrate bindingUniRule annotation2
Regioni143 – 147Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3C Bacteria
COG0596 LUCA
HOGENOMiHOG000028062
KOiK02170
OMAiLHGWGMN

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_01260 Carboxylester, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000073 AB_hydrolase_1
IPR010076 BioH
PfamiView protein in Pfam
PF00561 Abhydrolase_1, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR01738 bioH, 1 hit

Sequencei

Sequence statusi: Complete.

Q74Y45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQLYWYTCG EGDCDLVLLH GWGLNSGVWH CIIDRLAPHF RLHLVDLPGY
60 70 80 90 100
GRSQDYGAMS LADMAERVAQ QAPKQALWLG WSMGGLVASQ IALSQPECVR
110 120 130 140 150
GLITVSSSPC FTARDEWPGI KPEVLAGFQH QLSDDFHRTV ERFLALQTLG
160 170 180 190 200
TESSRQDARL LKSVVLQHQM PDVEVLTGGL AILRTADLRT ALAGFTLPFM
210 220 230 240 250
RVYGHLDSLV PRKVASLLDS AWPQTQSVVM QGAAHAPFIS HPNDFAKLIL

NFAEENKK
Length:258
Mass (Da):28,590
Last modified:August 16, 2005 - v2
Checksum:i5866E222A2C6D145
GO

Sequence cautioni

The sequence AAM87452 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS60408 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL18815.1
AE009952 Genomic DNA Translation: AAM87452.1 Different initiation.
AE017042 Genomic DNA Translation: AAS60408.1 Different initiation.
PIRiAF0016
RefSeqiWP_002208922.1, NZ_PDBR01000008.1
YP_002345215.1, NC_003143.1

Genome annotation databases

EnsemblBacteriaiAAM87452; AAM87452; y3908
AAS60408; AAS60408; YP_0130
GeneIDi1172975
KEGGiype:YPO0129
ypk:y3908
ypm:YP_0130
PATRICifig|1028802.3.peg.151

Similar proteinsi

Entry informationi

Entry nameiBIOH_YERPE
AccessioniPrimary (citable) accession number: Q74Y45
Secondary accession number(s): Q0WKH3, Q8CZK1, Q8ZJH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: March 28, 2018
This is version 98 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health