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Protein

Bifunctional protein Aas

Gene

aas

Organism
Yersinia pestis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Plays a role in lysophospholipid acylation. Transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3-phosphoethanolamine (2-acyl-GPE) formed by transacylation reactions or degradation by phospholipase A1.UniRule annotation

Catalytic activityi

Acyl-[acyl-carrier-protein] + O-(2-acyl-sn-glycero-3-phospho)ethanolamine = [acyl-carrier-protein] + O-(1-beta-acyl-2-acyl-sn-glycero-3-phospho)ethanolamine.UniRule annotation
ATP + a long-chain fatty acid + an [acyl-carrier-protein] = AMP + diphosphate + a long-chain acyl-[acyl-carrier-protein].UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei36UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Ligase, Multifunctional enzyme, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein AasUniRule annotation
Including the following 2 domains:
2-acylglycerophosphoethanolamine acyltransferaseUniRule annotation (EC:2.3.1.40UniRule annotation)
Alternative name(s):
2-acyl-GPE acyltransferaseUniRule annotation
Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferaseUniRule annotation
Acyl-[acyl-carrier-protein] synthetaseUniRule annotation (EC:6.2.1.20UniRule annotation)
Alternative name(s):
Acyl-ACP synthetaseUniRule annotation
Long-chain-fatty-acid--[acyl-carrier-protein] ligaseUniRule annotation
Gene namesi
Name:aasUniRule annotation
Ordered Locus Names:YPO0793, y3181, YP_2864
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei258 – 277HelicalUniRule annotationAdd BLAST20
Transmembranei409 – 433HelicalUniRule annotationAdd BLAST25

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001930551 – 718Bifunctional protein AasAdd BLAST718

Proteomic databases

PaxDbiQ74RZ6

Interactioni

Protein-protein interaction databases

IntActiQ74RZ6, 2 interactors
STRINGi187410.y3181

Structurei

3D structure databases

ProteinModelPortaliQ74RZ6
SMRiQ74RZ6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 138AcyltransferaseAdd BLAST124
Regioni233 – 646AMP-bindingAdd BLAST414

Sequence similaritiesi

In the N-terminal section; belongs to the 2-acyl-GPE acetyltransferase family.UniRule annotation
In the C-terminal section; belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CEY Bacteria
COG0204 LUCA
COG0318 LUCA
HOGENOMiHOG000004907
KOiK05939
OMAiCGCLNDN

Family and domain databases

HAMAPiMF_01162 Aas, 1 hit
InterProiView protein in InterPro
IPR023775 Aas
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF01553 Acyltransferase, 1 hit
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Q74RZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYRLLRALF RGLFRVTIDG VTDQFKHEKL IITPNHVSFL DGALLALFLP
60 70 80 90 100
IKPVFAVYTS ITDTWYMRWL KPYVDFVALD PTNPMAIKHL VRMVEQGRPV
110 120 130 140 150
VIFPEGRITV TGSLMKIYDG AAFVAAKSGA AVVPIRLDGP EFTHFGRLQG
160 170 180 190 200
VLKTRWFPKI SIHVLPATTI PMPQAPRSRE RRVLAGEHLH TIMMAARMAT
210 220 230 240 250
VPRETLFEAL LSAQTRYGRF KPCIEDVSFK EDSYQTLLKK TLGVSRILQR
260 270 280 290 300
FTVPGEHVGM LLPNATITAA AIFGASLRGR IPALLNYTSG AKGLQSAIIA
310 320 330 340 350
ASLKTIVTSR QFLEKGKLTH LPEQVNEVNW VYLEDLKDTV TLTDKLWILF
360 370 380 390 400
HLCFPRRAML PQQADGSALI LFTSGSEGNP KGVVHSHASL LANVEQIRTI
410 420 430 440 450
ADFTPRDRFM SSLPLFHAFG LTVGLFTPLM TGSRVFLYPS PLHYRVVPEL
460 470 480 490 500
VYDRNCTVLF GTSTFLGNYA RFAHPYDFAR VRYVVAGAEK LAESTKQIWQ
510 520 530 540 550
DKFGIRILEG YGVTECAPVV AINVPMAAKV NTVGRILPGM EARLINVPGI
560 570 580 590 600
AQGGRLQLRG PNIMRGYLRV ENPGVLEQPS AENAQGELDA NWYDTGDIVT
610 620 630 640 650
LDEQGFCAIR GRVKRFAKLA GEMVSLESVE QLAISLSPEG QHAAAAKTDS
660 670 680 690 700
AKGEALVLFT TDSEITRERL IKVARENGVP ELAVPRDIRV VKALPLLGSG
710
KPDFVTLGKM AQDPEMSV
Length:718
Mass (Da):79,386
Last modified:April 26, 2005 - v2
Checksum:iC0AB5A05BBCB6C35
GO

Sequence cautioni

The sequence AAM86731 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti366G → D in AAS63046 (PubMed:15368893).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL19465.1
AE009952 Genomic DNA Translation: AAM86731.1 Different initiation.
AE017042 Genomic DNA Translation: AAS63046.1
PIRiAG0097
RefSeqiWP_002209843.1, NZ_PDBR01000013.1
YP_002345848.1, NC_003143.1

Genome annotation databases

EnsemblBacteriaiAAM86731; AAM86731; y3181
AAS63046; AAS63046; YP_2864
GeneIDi1173632
KEGGiype:YPO0793
ypk:y3181
ypm:YP_2864
PATRICifig|214092.21.peg.1055

Similar proteinsi

Entry informationi

Entry nameiAAS_YERPE
AccessioniPrimary (citable) accession number: Q74RZ6
Secondary accession number(s): Q0WIP0, Q8CZY1, Q8ZHU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: March 28, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health