Q74R94 (GPPA_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
EC=3.6.1.40

Alternative name(s):
Guanosine pentaphosphate phosphohydrolase
pppGpp-5'-phosphohydrolase

Gene names

Name:	gppA
Ordered Locus Names:	YPO3870, y0358, YP_3175

Organism

Yersinia pestis [Reference proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities By similarity. HAMAP-Rule MF_01550
Catalytic activity	Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate. HAMAP-Rule MF_01550
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2. HAMAP-Rule MF_01550
Sequence similarities	Belongs to the GppA/Ppx family. GppA subfamily.
Caution	In strain CO-92 it seems to be a pseudogene. It is interrupted by frameshifts in positions 90 and 143. The sequence has been verified by the authors and is believed to be correct.
Sequence caution	The sequence AE009952 differs from that shown. Reason: Frameshift at position 143.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	guanosine pentaphosphate catabolic process Inferred from electronic annotation. Source: InterPro guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway polyphosphate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	exopolyphosphatase activity Inferred from electronic annotation. Source: InterPro guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase HAMAP-Rule MF_01550		PRO_0000194297

Sequences

	Sequence		Length	Mass (Da)
	Q74R94 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 583A72F958AFFEB5	FASTA	498	55,926
	10 20 30 40 50 60 MMLSSTSLYA AIDLGSNSFH MLVVREVAGS IQTLARIKRK VRLAAGLDNQ NHLSQEAMER 70 80 90 100 110 120 GWQCLKLFSE RLQDIPLDQI RVVATATLRL ASNADEFLRT ATEILGCPIQ VISGEEEARL 130 140 150 160 170 180 IYHGVAHTTG GPEQRLVVDI GGGSTELVTG NGAQANILVS LSMGCVTWLE RYFGDRHLAK 190 200 210 220 230 240 ENFERAELAA HEMIKPVAQR FREHGWQVCV GASGTVQALQ EIMVAQGMDE LITLAKLQQL 250 260 270 280 290 300 KQRAIQCGKL EELEIPGLTL ERALVFPSGL SILIAIFQEL SIESMTLAGG ALREGLVYGM 310 320 330 340 350 360 LHLPVEQDIR RRTLRNLQRR YLLDTEQAKR VSCLADNFFL QVEKEWHLDG RCREFLQNAC 370 380 390 400 410 420 LIHEIGLSVD FKHAPQHAAY LIRNLDLPGF TPAQKLLLSA LLQNQSDTID LSLLNQQNAL 430 440 450 460 470 480 PADMAQHLCR LLRLAIIFSS RRRDDTLPAV RLRADNNALY VLVPQGWLEQ HPYRAEALEQ 490 ESHWQSYVQW PLLLEELS

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM10+ / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. No translation available. AE009952 Genomic DNA. No translation available. AE017042 Genomic DNA. Translation: AAS63343.1.
RefSeq	NP_994466.1. NC_005810.1.
3D structure databases
ProteinModelPortal	Q74R94.
ModBase	Search...
Protein-protein interaction databases
STRING	229193.YP_3175.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS63343; AAS63343; YP_3175.
GeneID	2765920.
KEGG	ypm:YP_3175.
PATRIC	18625474. VBIYerPes99487_3635.
Phylogenomic databases
eggNOG	COG0248.
HOGENOM	HOG000258672.
KO	K01524.
OMA	PPRVAEH.
ProtClustDB	PRK11031.
Enzyme and pathway databases
UniPathway	UPA00908; UER00884.
Family and domain databases
HAMAP	MF_01550. GppA.
InterPro	IPR022371. Exopolyphosphatase. IPR023709. Guo-5TP_3DP_PyrP. IPR003695. Ppx_GppA. [Graphical view]
Pfam	PF02541. Ppx-GppA. 1 hit. [Graphical view]
TIGRFAMs	TIGR03706. exo_poly_only. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPPA_YERPE

Accession

Primary (citable) accession number: Q74R94

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program