Q74PY8 (TYPH_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine phosphorylase
EC=2.4.2.4

Alternative name(s):
TdRPase

Gene names

Name:	deoA
Ordered Locus Names:	YPO0437, y3742, YP_3744

Organism

Yersinia pestis [Reference proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The enzymes which catalyze the reversible phosphorolysis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis By similarity. HAMAP-Rule MF_01628
Catalytic activity	Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01628
Pathway	Pyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2. HAMAP-Rule MF_01628
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
Caution	In strains CO-92 and KIM5 it could be the product of a pseudogene.
Sequence caution	The sequence AE009952 differs from that shown. Reason: Frameshift at position 146. The sequence AL590842 differs from that shown. Reason: Frameshift at position 146.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pyrimidine nucleobase metabolic process Inferred from electronic annotation. Source: InterPro thymidine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	phosphorylase activity Inferred from electronic annotation. Source: InterPro pyrimidine-nucleoside phosphorylase activity Inferred from electronic annotation. Source: InterPro thymidine phosphorylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Thymidine phosphorylase HAMAP-Rule MF_01628		PRO_0000059075
Experimental info
	Sequence conflict	413	1	E → A Ref.1
	Sequence conflict	413	1	E → A Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q74PY8 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: 03E23E2C16C1659D	FASTA	440	46,975
	10 20 30 40 50 60 MFLAQEIIRK KRDGQPLSEE EIRFFINGIR DNVVSEGQIA ALAMTIYFHD MSMPERVALT 70 80 90 100 110 120 MAMRDSGTVL NWKSLNLNGP LVDKHSTGGV GDVTSLMLGP MVAACGGYVP MISGRGLGHT 130 140 150 160 170 180 GGTLDKLEAI PGFDIFPDDN AFRKIIQNVG VAIIGQTSSL APADKRFYAT RDITATVDSI 190 200 210 220 230 240 PLITASILAK KLAEGLDALV MDVKVGSGAF MPTYSLSADL AQAIVGVANG AGCKTTALLT 250 260 270 280 290 300 DMNQVLASSA GNGVEVREAV RFLTGEYRNP RLLEVTMALC VEMLLSGGLA HDEADARAKL 310 320 330 340 350 360 QAVLDNGKAA EVFGRMVAAQ KGPADFVERY DSYLPVATLS KPVFAEQTGI ITAMDTRALG 370 380 390 400 410 420 MAVVALGGGR RRATDPIDYS VGLTEMARLG TRVDGQQPLA VIHANNEDDW QQEAEVVRAA 430 440 ITLGNNTPEE TPVIYRRITE

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM10+ / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. No translation available. AE009952 Genomic DNA. No translation available. AE017042 Genomic DNA. Translation: AAS63892.1.
3D structure databases
ProteinModelPortal	Q74PY8.
SMR	Q74PY8. Positions 1-440.
ModBase	Search...
Protein-protein interaction databases
STRING	229193.YP_3744.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS63892; AAS63892; YP_3744.
Phylogenomic databases
eggNOG	COG0213.
HOGENOM	HOG000047313.
OMA	LMAIYFR.
ProtClustDB	PRK05820.
Enzyme and pathway databases
UniPathway	UPA00578; UER00638.
Family and domain databases
Gene3D	3.40.1030.10. 1 hit. 3.90.1170.30. 1 hit.
HAMAP	MF_01628. Thymid_phosp.
InterPro	IPR000312. Glycosyl_Trfase_fam3. IPR017459. Glycosyl_Trfase_fam3_N_dom. IPR013102. PYNP_C. IPR018090. Pyrmidine_PPas_bac/euk. IPR000053. Pyrmidine_PPase. IPR017872. Pyrmidine_PPase_CS. IPR013465. Thymidine_Pase. [Graphical view]
PANTHER	PTHR10515. PTHR10515. 1 hit.
Pfam	PF02885. Glycos_trans_3N. 1 hit. PF00591. Glycos_transf_3. 1 hit. PF07831. PYNP_C. 1 hit. [Graphical view]
PIRSF	PIRSF000478. TP_PyNP. 1 hit.
SMART	SM00941. PYNP_C. 1 hit. [Graphical view]
SUPFAM	SSF47648. Glyco_trans_3. 1 hit. SSF52418. Glyco_trans_3. 1 hit. SSF54680. PYNP_C. 1 hit.
TIGRFAMs	TIGR02643. T_phosphoryl. 1 hit. TIGR02644. Y_phosphoryl. 1 hit.
PROSITE	PS00647. THYMID_PHOSPHORYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYPH_YERPE

Accession

Primary (citable) accession number: Q74PY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	January 10, 2006
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program