Reviewed, UniProtKB/Swiss-Prot Q74N29 (DP2L_NANEQ)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	NEQ420

Organism

Nanoarchaeum equitans [Complete proteome] [HAMAP]

Taxonomic identifier

160232 [NCBI]

Taxonomic lineage

Archaea › Nanoarchaeota › Nanoarchaeum

Protein attributes

Sequence length	1243 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1243	1243	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000294697

Sequences

	Sequence		Length	Mass (Da)
	Q74N29-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A48088166C1CF516	FASTA	1,243	142,593
	10 20 30 40 50 60 MVYYDILAKE LEKLYEIAEK ARAQGYDPKD IVEIPLANNM GERVAHLISA IHEQLDVKKT 70 80 90 100 110 120 AELILELEKQ YGFLDWRVGL KLIDYALEGK LIPYDDILKR IDLGVRLALG YITMGVVSAP 130 140 150 160 170 180 LEGLVELRIK KRKDGKPYLA LYYAGPIRGA GGTAAAVSVL FADYARKKAG LYEYDPTKEE 190 200 210 220 230 240 IERYKIEIDS YHNKVARLQY KPSDEEIEFL VKHIPVEING DPTSDKEVPS YKNLERVETN 250 260 270 280 290 300 RIRGGMCLVI AEGIAQKAKK IYGKITEWGK EFGLEHWEFL GEYLKLQKEI QAKLAAESGG 310 320 330 340 350 360 EIEGKEIKRK ITLFLDEIKK EEITVKYPTP AGKYLQEITA GRPIFSLPMA IGGFRLRYGR 370 380 390 400 410 420 SFATGFATSG IHPATMFLTY GFLAIGTQMR GERPKKSTIV TPVTSLHPPI VKLKDGTVKK 430 440 450 460 470 480 VHSVVEAIKI ANDVEEILFL GDILTAFGDW LNEKANLVPS PYVEEWWIQE FDRAAMKKTD 490 500 510 520 530 540 YKIEFDVMKP RKLFKIEGLE NLSQYLGIPK DRLEEIIRKP FSRKPTIDEA ITISEKLGIP 550 560 570 580 590 600 LHPEYTPFWV HITKEELIKL IEELRKAKIQ NNKIYFHESQ KELKAILEKL FIEHFREGEY 610 620 630 640 650 660 FYINESMTKS LLYQLDNLNL EKAKKEFDNA IDALDLVNRL SPIEIRDVAG VYVGFRAGRP 670 680 690 700 710 720 EKAKMREMTG RPHGLFPVGE EGGKMRNVIE AYNKGYVKAE FALYYCNHCK RYTIYRKCEV 730 740 750 760 770 780 CGNKTKEVYV CKEIINRLKT SLFKQYKDWK KVEEEIASHL WAEAVTHSKN NCREPKRYSL 790 800 810 820 830 840 IKLNIKHYVD RAVQKLKLFN IPKLVKGVRG TSNKDHIVER LEKAFLRSVS DVYVNKDGTI 850 860 870 880 890 900 RYDGTQIPLT HFRPKDLIGV TIEKLKELGY THDIYGNPLE REDQILQLKP QDIILPYGIL 910 920 930 940 950 960 VKSRLNKNKI VRTDAAEAFK KVANFVDEEL KRLYNLEPYY NIEKAEDLIG KIVFGIAPHT 970 980 990 1000 1010 1020 SAAVVGRIIG FTPIQGIIAH PIWHAGQRRN CDGDETAVML GLDALINFSR EYLPDKRGAR 1030 1040 1050 1060 1070 1080 TMDAPLVLTL KLELKAVDDE VHDMDIVYNY PLEFYYETLK GSPAKDVKKQ CGILTLGDFL 1090 1100 1110 1120 1130 1140 KEEDISKIPI GFTHPTKSIR LGNKVSLYKR LKTMQQKTEW QLKLAERIRA VDENIVAEIV 1150 1160 1170 1180 1190 1200 IEKHFMPDIK GNLRGFSSQV FRCTKCDTTY DRVPLSGKCP KCGGNIVFTV HEGTIKKYLP 1210 1220 1230 1240 TSLYLARKYK IKKFTKQSLF LLDRRIKQIF GGEKKSLADF INS

References

[1]

"The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism." Waters E., Hohn M.J., Ahel I., Graham D.E., Adams M.D., Barnstead M., Beeson K.Y., Bibbs L., Bolanos R., Keller M., Kretz K., Lin X., Mathur E., Ni J., Podar M., Richardson T., Sutton G.G., Simon M. , Soell D., Stetter K.O., Short J.M., Noorderwier M. Proc. Natl. Acad. Sci. U.S.A. 100:12984-12988(2003) [PubMed: 14566062] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Kin4-M.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017199 Genomic DNA. Translation: AAR39264.1.
RefSeq	NP_963703.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2732644.
GenomeReviews	Gene locus NEQ420 in contig AE017199_GR.
KEGG	neq:NEQ420.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497074.
OMA	NCDGDED.
Enzyme and pathway databases
BRENDA	2.7.7.7. 278551.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_NANEQ

Accession

Primary (citable) accession number: Q74N29

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)