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Q74MX2

- AGOG_NANEQ

UniProt

Q74MX2 - AGOG_NANEQ

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Protein

N-glycosylase/DNA lyase

Gene

NEQ515

Organism
Nanoarchaeum equitans (strain Kin4-M)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 2718-oxoguanineUniRule annotation
Binding sitei48 – 4818-oxoguanine; via carbonyl oxygenUniRule annotation
Binding sitei59 – 5918-oxoguanineUniRule annotation
Active sitei130 – 1301Schiff-base intermediate with DNAUniRule annotation
Binding sitei134 – 13418-oxoguanineUniRule annotation
Binding sitei160 – 16018-oxoguanine; via carbonyl oxygenUniRule annotation
Active sitei162 – 1621UniRule annotation
Binding sitei190 – 19018-oxoguanineUniRule annotation
Binding sitei194 – 19418-oxoguanineUniRule annotation

GO - Molecular functioni

  1. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  2. oxidized base lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA excision, DNA repair

Enzyme and pathway databases

BioCyciNEQU228908:GJB6-543-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyaseUniRule annotation
Alternative name(s):
8-oxoguanine DNA glycosylaseUniRule annotation (EC:3.2.2.-UniRule annotation)
AGOGUniRule annotation
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
Short name:
AP lyaseUniRule annotation
Gene namesi
Ordered Locus Names:NEQ515
OrganismiNanoarchaeum equitans (strain Kin4-M)
Taxonomic identifieri228908 [NCBI]
Taxonomic lineageiArchaeaNanoarchaeotaNanoarchaeum
ProteomesiUP000000578: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216N-glycosylase/DNA lyasePRO_0000185111Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi228908.NEQ515.

Structurei

3D structure databases

ProteinModelPortaliQ74MX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni106 – 17065Helix-hairpin-helixAdd
BLAST

Domaini

Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).

Sequence similaritiesi

Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.UniRule annotation

Phylogenomic databases

eggNOGiCOG4047.
HOGENOMiHOG000254352.
KOiK01741.
OMAiREHYYEN.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_01168. AGOG.
InterProiIPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view]
PfamiPF09171. DUF1886. 1 hit.
[Graphical view]
PIRSFiPIRSF008955. AGOG. 1 hit.
SUPFAMiSSF48150. SSF48150. 1 hit.

Sequencei

Sequence statusi: Complete.

Q74MX2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDPLIKILK QFSIEDAKYV EYNLDRQFLA LKENPKPVGL VIANALISYQ
60 70 80 90 100
LTMPGERYWE LFAKKVNSFN DLYDFVKKYN PRFLSNKLKR LERFKPYIDI
110 120 130 140 150
IEQNREHYYE NMVALNKFLA KIMNQNIYDK TIVFSIKMFA YAMRALGYKF
160 170 180 190 200
KPFPFEIAIP LDYRLKKINP DLNYWFYVSK QTNIPPLHID SLIWPIFRIK
210
NLPKKFALLK EYLSNL
Length:216
Mass (Da):26,005
Last modified:July 5, 2004 - v1
Checksum:iDC1D0C211503B3FA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017199 Genomic DNA. Translation: AAR39356.1.
RefSeqiNP_963795.1. NC_005213.1.
WP_011153495.1. NC_005213.1.

Genome annotation databases

EnsemblBacteriaiAAR39356; AAR39356; NEQ515.
GeneIDi2654177.
KEGGineq:NEQ515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017199 Genomic DNA. Translation: AAR39356.1 .
RefSeqi NP_963795.1. NC_005213.1.
WP_011153495.1. NC_005213.1.

3D structure databases

ProteinModelPortali Q74MX2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 228908.NEQ515.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAR39356 ; AAR39356 ; NEQ515 .
GeneIDi 2654177.
KEGGi neq:NEQ515.

Phylogenomic databases

eggNOGi COG4047.
HOGENOMi HOG000254352.
KOi K01741.
OMAi REHYYEN.

Enzyme and pathway databases

BioCyci NEQU228908:GJB6-543-MONOMER.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPi MF_01168. AGOG.
InterProi IPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view ]
Pfami PF09171. DUF1886. 1 hit.
[Graphical view ]
PIRSFi PIRSF008955. AGOG. 1 hit.
SUPFAMi SSF48150. SSF48150. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Kin4-M.

Entry informationi

Entry nameiAGOG_NANEQ
AccessioniPrimary (citable) accession number: Q74MX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 5, 2004
Last modified: October 1, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3