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Q74MX2

- AGOG_NANEQ

UniProt

Q74MX2 - AGOG_NANEQ

Protein

N-glycosylase/DNA lyase

Gene

NEQ515

Organism
Nanoarchaeum equitans (strain Kin4-M)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.UniRule annotation

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei27 – 2718-oxoguanineUniRule annotation
    Binding sitei48 – 4818-oxoguanine; via carbonyl oxygenUniRule annotation
    Binding sitei59 – 5918-oxoguanineUniRule annotation
    Active sitei130 – 1301Schiff-base intermediate with DNAUniRule annotation
    Binding sitei134 – 13418-oxoguanineUniRule annotation
    Binding sitei160 – 16018-oxoguanine; via carbonyl oxygenUniRule annotation
    Active sitei162 – 1621UniRule annotation
    Binding sitei190 – 19018-oxoguanineUniRule annotation
    Binding sitei194 – 19418-oxoguanineUniRule annotation

    GO - Molecular functioni

    1. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
    2. oxidized base lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. base-excision repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA excision, DNA repair

    Enzyme and pathway databases

    BioCyciNEQU228908:GJB6-543-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-glycosylase/DNA lyaseUniRule annotation
    Alternative name(s):
    8-oxoguanine DNA glycosylaseUniRule annotation (EC:3.2.2.-UniRule annotation)
    AGOGUniRule annotation
    DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
    Short name:
    AP lyaseUniRule annotation
    Gene namesi
    Ordered Locus Names:NEQ515
    OrganismiNanoarchaeum equitans (strain Kin4-M)
    Taxonomic identifieri228908 [NCBI]
    Taxonomic lineageiArchaeaNanoarchaeotaNanoarchaeum
    ProteomesiUP000000578: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 216216N-glycosylase/DNA lyasePRO_0000185111Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi228908.NEQ515.

    Structurei

    3D structure databases

    ProteinModelPortaliQ74MX2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni106 – 17065Helix-hairpin-helixAdd
    BLAST

    Domaini

    Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).

    Sequence similaritiesi

    Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG4047.
    HOGENOMiHOG000254352.
    KOiK01741.
    OMAiREHYYEN.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPiMF_01168. AGOG.
    InterProiIPR011257. DNA_glycosylase.
    IPR023170. HTH_base_excis_C.
    IPR015254. N-Glyclase/DNA_lyase-like_arc.
    IPR016544. N-Glyclase/DNA_lyase_arc.
    [Graphical view]
    PfamiPF09171. DUF1886. 1 hit.
    [Graphical view]
    PIRSFiPIRSF008955. AGOG. 1 hit.
    SUPFAMiSSF48150. SSF48150. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q74MX2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDPLIKILK QFSIEDAKYV EYNLDRQFLA LKENPKPVGL VIANALISYQ    50
    LTMPGERYWE LFAKKVNSFN DLYDFVKKYN PRFLSNKLKR LERFKPYIDI 100
    IEQNREHYYE NMVALNKFLA KIMNQNIYDK TIVFSIKMFA YAMRALGYKF 150
    KPFPFEIAIP LDYRLKKINP DLNYWFYVSK QTNIPPLHID SLIWPIFRIK 200
    NLPKKFALLK EYLSNL 216
    Length:216
    Mass (Da):26,005
    Last modified:July 5, 2004 - v1
    Checksum:iDC1D0C211503B3FA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017199 Genomic DNA. Translation: AAR39356.1.
    RefSeqiNP_963795.1. NC_005213.1.
    WP_011153495.1. NC_005213.1.

    Genome annotation databases

    EnsemblBacteriaiAAR39356; AAR39356; NEQ515.
    GeneIDi2654177.
    KEGGineq:NEQ515.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017199 Genomic DNA. Translation: AAR39356.1 .
    RefSeqi NP_963795.1. NC_005213.1.
    WP_011153495.1. NC_005213.1.

    3D structure databases

    ProteinModelPortali Q74MX2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 228908.NEQ515.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAR39356 ; AAR39356 ; NEQ515 .
    GeneIDi 2654177.
    KEGGi neq:NEQ515.

    Phylogenomic databases

    eggNOGi COG4047.
    HOGENOMi HOG000254352.
    KOi K01741.
    OMAi REHYYEN.

    Enzyme and pathway databases

    BioCyci NEQU228908:GJB6-543-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPi MF_01168. AGOG.
    InterProi IPR011257. DNA_glycosylase.
    IPR023170. HTH_base_excis_C.
    IPR015254. N-Glyclase/DNA_lyase-like_arc.
    IPR016544. N-Glyclase/DNA_lyase_arc.
    [Graphical view ]
    Pfami PF09171. DUF1886. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF008955. AGOG. 1 hit.
    SUPFAMi SSF48150. SSF48150. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Kin4-M.

    Entry informationi

    Entry nameiAGOG_NANEQ
    AccessioniPrimary (citable) accession number: Q74MX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3