Reviewed, UniProtKB/Swiss-Prot Q74MX2 (AGOG_NANEQ)

Names and origin

Protein names

Recommended name:
    N-glycosylase/DNA lyase
Alternative name(s):
    AGOG
    8-oxoguanine DNA glycosylase
    EC=3.2.2.-
    DNA-(apurinic or apyrimidinic site) lyase
      Short name=AP lyase
    EC=4.2.99.18

Gene names

Ordered Locus Names:

NEQ515

Organism

Nanoarchaeum equitans [Complete proteome] [HAMAP]

Taxonomic identifier

160232 [NCBI]

Taxonomic lineage

Archaea › Nanoarchaeota › Nanoarchaeum

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates By similarity. HAMAP MF_01168
Catalytic activity	The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP MF_01168
Domain	Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif). HAMAP MF_01168
Sequence similarities	Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.

Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair
Molecular function	Hydrolase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	base-excision repair Inferred from electronic annotation. Source: HAMAP
Molecular function	DNA-(apurinic or apyrimidinic site) lyase activity Inferred from electronic annotation. Source: HAMAP oxidized base lesion DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	N-glycosylase/DNA lyase HAMAP MF_01168		PRO_0000185111
Regions
	Region	106 – 170	65	Helix-hairpin-helix HAMAP MF_01168
Sites
	Active site	130	1	Schiff-base intermediate with DNA By similarity
	Active site	162	1	Potential
	Binding site	27	1	8-oxoguanine By similarity
	Binding site	48	1	8-oxoguanine; via carbonyl oxygen By similarity
	Binding site	59	1	8-oxoguanine By similarity
	Binding site	134	1	8-oxoguanine By similarity
	Binding site	160	1	8-oxoguanine; via carbonyl oxygen By similarity
	Binding site	190	1	8-oxoguanine By similarity
	Binding site	194	1	8-oxoguanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q74MX2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: DC1D0C211503B3FA	FASTA	216	26,005
	10 20 30 40 50 60 MEDPLIKILK QFSIEDAKYV EYNLDRQFLA LKENPKPVGL VIANALISYQ LTMPGERYWE 70 80 90 100 110 120 LFAKKVNSFN DLYDFVKKYN PRFLSNKLKR LERFKPYIDI IEQNREHYYE NMVALNKFLA 130 140 150 160 170 180 KIMNQNIYDK TIVFSIKMFA YAMRALGYKF KPFPFEIAIP LDYRLKKINP DLNYWFYVSK 190 200 210 QTNIPPLHID SLIWPIFRIK NLPKKFALLK EYLSNL

References

[1]

"The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism." Waters E., Hohn M.J., Ahel I., Graham D.E., Adams M.D., Barnstead M., Beeson K.Y., Bibbs L., Bolanos R., Keller M., Kretz K., Lin X., Mathur E., Ni J., Podar M., Richardson T., Sutton G.G., Simon M. , Soell D., Stetter K.O., Short J.M., Noorderwier M. Proc. Natl. Acad. Sci. U.S.A. 100:12984-12988(2003) [PubMed: 14566062] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Kin4-M.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017199 Genomic DNA. Translation: AAR39356.1.
RefSeq	NP_963795.1.
3D structure databases
SMR	Q74MX2. Positions 4-198.
ModBase	Search...
Genome annotation databases
GeneID	2654177.
GenomeReviews	Gene locus NEQ515 in contig AE017199_GR.
KEGG	neq:NEQ515.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG539606.
Enzyme and pathway databases
BRENDA	4.2.99.18. 278551.
Family and domain databases
HAMAP	MF_01168. AGOG. [Tree]
InterPro	IPR015254. N-Glyclase/DNA_lyase-like_arc. IPR016544. N-Glyclase/DNA_lyase_arc. [Graphical view]
Pfam	PF09171. DUF1886. 1 hit. [Graphical view]
PIRSF	PIRSF008955. AGOG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AGOG_NANEQ

Accession

Primary (citable) accession number: Q74MX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)