SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q74MX2

- AGOG_NANEQ

UniProt

Q74MX2 - AGOG_NANEQ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

N-glycosylase/DNA lyase

Gene
NEQ515
Organism
Nanoarchaeum equitans (strain Kin4-M)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates By similarity.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 2718-oxoguanine By similarity
Binding sitei48 – 4818-oxoguanine; via carbonyl oxygen By similarity
Binding sitei59 – 5918-oxoguanine By similarity
Active sitei130 – 1301Schiff-base intermediate with DNA By similarity
Binding sitei134 – 13418-oxoguanine By similarity
Binding sitei160 – 16018-oxoguanine; via carbonyl oxygen By similarity
Active sitei162 – 1621 Reviewed prediction
Binding sitei190 – 19018-oxoguanine By similarity
Binding sitei194 – 19418-oxoguanine By similarity

GO - Molecular functioni

  1. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  2. oxidized base lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA excision, DNA repair

Enzyme and pathway databases

BioCyciNEQU228908:GJB6-543-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyase
Alternative name(s):
8-oxoguanine DNA glycosylase (EC:3.2.2.-)
AGOG
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Short name:
AP lyase
Gene namesi
Ordered Locus Names:NEQ515
OrganismiNanoarchaeum equitans (strain Kin4-M)
Taxonomic identifieri228908 [NCBI]
Taxonomic lineageiArchaeaNanoarchaeotaNanoarchaeum
ProteomesiUP000000578: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216N-glycosylase/DNA lyaseUniRule annotationPRO_0000185111Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi228908.NEQ515.

Structurei

3D structure databases

ProteinModelPortaliQ74MX2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni106 – 17065Helix-hairpin-helixUniRule annotationAdd
BLAST

Domaini

Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4047.
HOGENOMiHOG000254352.
KOiK01741.
OMAiREHYYEN.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_01168. AGOG.
InterProiIPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view]
PfamiPF09171. DUF1886. 1 hit.
[Graphical view]
PIRSFiPIRSF008955. AGOG. 1 hit.
SUPFAMiSSF48150. SSF48150. 1 hit.

Sequencei

Sequence statusi: Complete.

Q74MX2-1 [UniParc]FASTAAdd to Basket

« Hide

MEDPLIKILK QFSIEDAKYV EYNLDRQFLA LKENPKPVGL VIANALISYQ    50
LTMPGERYWE LFAKKVNSFN DLYDFVKKYN PRFLSNKLKR LERFKPYIDI 100
IEQNREHYYE NMVALNKFLA KIMNQNIYDK TIVFSIKMFA YAMRALGYKF 150
KPFPFEIAIP LDYRLKKINP DLNYWFYVSK QTNIPPLHID SLIWPIFRIK 200
NLPKKFALLK EYLSNL 216
Length:216
Mass (Da):26,005
Last modified:July 5, 2004 - v1
Checksum:iDC1D0C211503B3FA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017199 Genomic DNA. Translation: AAR39356.1.
RefSeqiNP_963795.1. NC_005213.1.
WP_011153495.1. NC_005213.1.

Genome annotation databases

EnsemblBacteriaiAAR39356; AAR39356; NEQ515.
GeneIDi2654177.
KEGGineq:NEQ515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017199 Genomic DNA. Translation: AAR39356.1 .
RefSeqi NP_963795.1. NC_005213.1.
WP_011153495.1. NC_005213.1.

3D structure databases

ProteinModelPortali Q74MX2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 228908.NEQ515.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAR39356 ; AAR39356 ; NEQ515 .
GeneIDi 2654177.
KEGGi neq:NEQ515.

Phylogenomic databases

eggNOGi COG4047.
HOGENOMi HOG000254352.
KOi K01741.
OMAi REHYYEN.

Enzyme and pathway databases

BioCyci NEQU228908:GJB6-543-MONOMER.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPi MF_01168. AGOG.
InterProi IPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view ]
Pfami PF09171. DUF1886. 1 hit.
[Graphical view ]
PIRSFi PIRSF008955. AGOG. 1 hit.
SUPFAMi SSF48150. SSF48150. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Kin4-M.

Entry informationi

Entry nameiAGOG_NANEQ
AccessioniPrimary (citable) accession number: Q74MX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3