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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Nanoarchaeum equitans (strain Kin4-M)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNEQU228908:GJB6-320-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:NEQ302
OrganismiNanoarchaeum equitans (strain Kin4-M)
Taxonomic identifieri228908 [NCBI]
Taxonomic lineageiArchaeaNanoarchaeotaNanoarchaeum
ProteomesiUP000000578 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 562562Glutamate--tRNA ligasePRO_0000119723Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi228908.NEQ302.

Structurei

3D structure databases

ProteinModelPortaliQ74MU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi90 – 10011"HIGH" regionAdd
BLAST

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q74MU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKEIIAYLK LKYGKVKPQS VIGKIIAKNP ELKAKIKELI PIIEQEIKEV
60 70 80 90 100
EQMDIEQIKE LAKAYEQTIE KKEKDIEIPV KDKVITRFAP NPSGLLHIGH
110 120 130 140 150
ARAIVLNHYI AKKYNGQFIL RIEDTDPDVK KPMIEAYELI PKDVEWLIQE
160 170 180 190 200
KPDKIVVQSD RLDIYYKYIE ELIKQGKAYV CLCKAEEFRE YRNKGLPCPH
210 220 230 240 250
RNQSIEENLE LWSKMLKGEF KKGEAVVRLK TDLTHKDPGV RDFPIARIVE
260 270 280 290 300
NPHPRIGYKP VFPLYNFAVV IDDHLLGITH VIRMKEHTNN AIKQSFIYKA
310 320 330 340 350
FGWEEPIYLE YGALLTDMPT HKSEIRKLIE QKQIEGWDDI RLPTLIALRR
360 370 380 390 400
RGILPEAIYE YIVKDVGLNK ADIKVDWNKI YYYHKIRIDK QTPRYFMIRD
410 420 430 440 450
PIAIVIENYD DIIDKFEIKN GIPYKKISKH PDVDLGYREL PIKEVLYIDK
460 470 480 490 500
RDAERLKQKP LRLFELFNIK FVGEIEIEYG DEYSKTKEKA YAVRVISFDV
510 520 530 540 550
QEAIKNKYPI VQWLPEYEET TLFELDGYKK AYVEPNIKEN YVHFIREGYY
560
KRENDKWIFS VK
Length:562
Mass (Da):66,631
Last modified:July 5, 2004 - v1
Checksum:i03E496D7DA5E6507
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017199 Genomic DNA. Translation: AAR39150.1.
RefSeqiNP_963589.1. NC_005213.1.

Genome annotation databases

EnsemblBacteriaiAAR39150; AAR39150; NEQ302.
GeneIDi2654363.
KEGGineq:NEQ302.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017199 Genomic DNA. Translation: AAR39150.1.
RefSeqiNP_963589.1. NC_005213.1.

3D structure databases

ProteinModelPortaliQ74MU5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi228908.NEQ302.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAR39150; AAR39150; NEQ302.
GeneIDi2654363.
KEGGineq:NEQ302.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.

Enzyme and pathway databases

BioCyciNEQU228908:GJB6-320-MONOMER.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Kin4-M.

Entry informationi

Entry nameiSYE_NANEQ
AccessioniPrimary (citable) accession number: Q74MU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: April 1, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.