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Q74MU5 (SYE_NANEQ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:NEQ302
OrganismNanoarchaeum equitans (strain Kin4-M) [Reference proteome] [HAMAP]
Taxonomic identifier228908 [NCBI]
Taxonomic lineageArchaeaNanoarchaeotaNanoarchaeum

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 562562Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119723

Regions

Motif90 – 10011"HIGH" region HAMAP-Rule MF_00022

Sequences

Sequence LengthMass (Da)Tools
Q74MU5 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 03E496D7DA5E6507

FASTA56266,631
        10         20         30         40         50         60 
MYKEIIAYLK LKYGKVKPQS VIGKIIAKNP ELKAKIKELI PIIEQEIKEV EQMDIEQIKE 

        70         80         90        100        110        120 
LAKAYEQTIE KKEKDIEIPV KDKVITRFAP NPSGLLHIGH ARAIVLNHYI AKKYNGQFIL 

       130        140        150        160        170        180 
RIEDTDPDVK KPMIEAYELI PKDVEWLIQE KPDKIVVQSD RLDIYYKYIE ELIKQGKAYV 

       190        200        210        220        230        240 
CLCKAEEFRE YRNKGLPCPH RNQSIEENLE LWSKMLKGEF KKGEAVVRLK TDLTHKDPGV 

       250        260        270        280        290        300 
RDFPIARIVE NPHPRIGYKP VFPLYNFAVV IDDHLLGITH VIRMKEHTNN AIKQSFIYKA 

       310        320        330        340        350        360 
FGWEEPIYLE YGALLTDMPT HKSEIRKLIE QKQIEGWDDI RLPTLIALRR RGILPEAIYE 

       370        380        390        400        410        420 
YIVKDVGLNK ADIKVDWNKI YYYHKIRIDK QTPRYFMIRD PIAIVIENYD DIIDKFEIKN 

       430        440        450        460        470        480 
GIPYKKISKH PDVDLGYREL PIKEVLYIDK RDAERLKQKP LRLFELFNIK FVGEIEIEYG 

       490        500        510        520        530        540 
DEYSKTKEKA YAVRVISFDV QEAIKNKYPI VQWLPEYEET TLFELDGYKK AYVEPNIKEN 

       550        560 
YVHFIREGYY KRENDKWIFS VK 

« Hide

References

[1]"The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism."
Waters E., Hohn M.J., Ahel I., Graham D.E., Adams M.D., Barnstead M., Beeson K.Y., Bibbs L., Bolanos R., Keller M., Kretz K., Lin X., Mathur E., Ni J., Podar M., Richardson T., Sutton G.G., Simon M. expand/collapse author list , Soell D., Stetter K.O., Short J.M., Noorderwier M.
Proc. Natl. Acad. Sci. U.S.A. 100:12984-12988(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Kin4-M.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017199 Genomic DNA. Translation: AAR39150.1.
RefSeqNP_963589.1. NC_005213.1.

3D structure databases

ProteinModelPortalQ74MU5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228908.NEQ302.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAR39150; AAR39150; NEQ302.
GeneID2654363.
KEGGneq:NEQ302.

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000073585.
KOK01885.
OMAENDESKC.

Enzyme and pathway databases

BioCycNEQU228908:GJB6-320-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_A. Glu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_NANEQ
AccessionPrimary (citable) accession number: Q74MU5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries