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Q74MP4

- ENDA_NANEQ

UniProt

Q74MP4 - ENDA_NANEQ

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Nanoarchaeum equitans (strain Kin4-M)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.UniRule annotation

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei86 – 861UniRule annotation
    Active sitei102 – 1021UniRule annotation
    Active sitei133 – 1331UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciNEQU228908:GJB6-219-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:NEQ205
    OrganismiNanoarchaeum equitans (strain Kin4-M)
    Taxonomic identifieri228908 [NCBI]
    Taxonomic lineageiArchaeaNanoarchaeotaNanoarchaeum
    ProteomesiUP000000578: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 154154tRNA-splicing endonucleasePRO_0000109474Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi228908.NEQ205.

    Structurei

    Secondary structure

    1
    154
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 63
    Beta strandi9 – 113
    Helixi19 – 2810
    Helixi43 – 5210
    Helixi56 – 6813
    Beta strandi72 – 765
    Helixi77 – 793
    Beta strandi81 – 866
    Turni94 – 963
    Beta strandi99 – 1024
    Beta strandi106 – 1116
    Beta strandi115 – 1173
    Helixi118 – 13013
    Beta strandi135 – 15218

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3IEYX-ray2.11A1-154[»]
    ProteinModelPortaliQ74MP4.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ74MP4.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000107823.
    KOiK01170.
    OMAiNMGYLRL.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    HAMAPiMF_01833. EndA_short.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR016442. tRNA_splic_arch_short.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005285. tRNA_splic_archaea. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q74MP4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIGYLFGNRV LVDDKELPLI EAYYLLDKGE LEVYEDDKKL SKEEFLKKCL    50
    TYDERFLIRY KAYKELRDKG YTLGTALKFG ADFRVYDIGV IPKKGKRSER 100
    EHSKWVLYPV SKDETFDFYE FASKNRVAHS TRKKLLLGIV SDKIEFIEVS 150
    WKKP 154
    Length:154
    Mass (Da):18,254
    Last modified:July 5, 2004 - v1
    Checksum:iAFC04DE8E9F9061E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017199 Genomic DNA. Translation: AAR39058.1.
    RefSeqiNP_963497.1. NC_005213.1.
    WP_011153199.1. NC_005213.1.

    Genome annotation databases

    EnsemblBacteriaiAAR39058; AAR39058; NEQ205.
    GeneIDi2654445.
    KEGGineq:NEQ205.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017199 Genomic DNA. Translation: AAR39058.1 .
    RefSeqi NP_963497.1. NC_005213.1.
    WP_011153199.1. NC_005213.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3IEY X-ray 2.11 A 1-154 [» ]
    ProteinModelPortali Q74MP4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 228908.NEQ205.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAR39058 ; AAR39058 ; NEQ205 .
    GeneIDi 2654445.
    KEGGi neq:NEQ205.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000107823.
    KOi K01170.
    OMAi NMGYLRL.

    Enzyme and pathway databases

    BioCyci NEQU228908:GJB6-219-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q74MP4.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    HAMAPi MF_01833. EndA_short.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR016442. tRNA_splic_arch_short.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005285. tRNA_splic_archaea. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Kin4-M.

    Entry informationi

    Entry nameiENDA_NANEQ
    AccessioniPrimary (citable) accession number: Q74MP4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3