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Q74MP4

- ENDA_NANEQ

UniProt

Q74MP4 - ENDA_NANEQ

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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Nanoarchaeum equitans (strain Kin4-M)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei86 – 861UniRule annotation
Active sitei102 – 1021UniRule annotation
Active sitei133 – 1331UniRule annotation

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciNEQU228908:GJB6-219-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:NEQ205
OrganismiNanoarchaeum equitans (strain Kin4-M)
Taxonomic identifieri228908 [NCBI]
Taxonomic lineageiArchaeaNanoarchaeotaNanoarchaeum
ProteomesiUP000000578: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 154154tRNA-splicing endonucleasePRO_0000109474Add
BLAST

Interactioni

Subunit structurei

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi228908.NEQ205.

Structurei

Secondary structure

1
154
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63
Beta strandi9 – 113
Helixi19 – 2810
Helixi43 – 5210
Helixi56 – 6813
Beta strandi72 – 765
Helixi77 – 793
Beta strandi81 – 866
Turni94 – 963
Beta strandi99 – 1024
Beta strandi106 – 1116
Beta strandi115 – 1173
Helixi118 – 13013
Beta strandi135 – 15218

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IEYX-ray2.11A1-154[»]
ProteinModelPortaliQ74MP4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ74MP4.

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000107823.
KOiK01170.
OMAiNMGYLRL.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01833. EndA_short.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q74MP4 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIGYLFGNRV LVDDKELPLI EAYYLLDKGE LEVYEDDKKL SKEEFLKKCL
60 70 80 90 100
TYDERFLIRY KAYKELRDKG YTLGTALKFG ADFRVYDIGV IPKKGKRSER
110 120 130 140 150
EHSKWVLYPV SKDETFDFYE FASKNRVAHS TRKKLLLGIV SDKIEFIEVS

WKKP
Length:154
Mass (Da):18,254
Last modified:July 5, 2004 - v1
Checksum:iAFC04DE8E9F9061E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017199 Genomic DNA. Translation: AAR39058.1.
RefSeqiNP_963497.1. NC_005213.1.

Genome annotation databases

EnsemblBacteriaiAAR39058; AAR39058; NEQ205.
GeneIDi2654445.
KEGGineq:NEQ205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017199 Genomic DNA. Translation: AAR39058.1 .
RefSeqi NP_963497.1. NC_005213.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3IEY X-ray 2.11 A 1-154 [» ]
ProteinModelPortali Q74MP4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 228908.NEQ205.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAR39058 ; AAR39058 ; NEQ205 .
GeneIDi 2654445.
KEGGi neq:NEQ205.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000107823.
KOi K01170.
OMAi NMGYLRL.

Enzyme and pathway databases

BioCyci NEQU228908:GJB6-219-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q74MP4.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
HAMAPi MF_01833. EndA_short.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMi SSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Kin4-M.

Entry informationi

Entry nameiENDA_NANEQ
AccessioniPrimary (citable) accession number: Q74MP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3