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Q74MP4 (ENDA_NANEQ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease
Gene names
Name:endA
Ordered Locus Names:NEQ205
OrganismNanoarchaeum equitans (strain Kin4-M) [Reference proteome] [HAMAP]
Taxonomic identifier228908 [NCBI]
Taxonomic lineageArchaeaNanoarchaeotaNanoarchaeum

Protein attributes

Sequence length154 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01833

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. HAMAP-Rule MF_01833

Subunit structure

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.

Sequence similarities

Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 154154tRNA-splicing endonuclease HAMAP-Rule MF_01833
PRO_0000109474

Sites

Active site861 By similarity
Active site1021 By similarity
Active site1331 By similarity

Secondary structure

........................... 154
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q74MP4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: AFC04DE8E9F9061E

FASTA15418,254
        10         20         30         40         50         60 
MIGYLFGNRV LVDDKELPLI EAYYLLDKGE LEVYEDDKKL SKEEFLKKCL TYDERFLIRY 

        70         80         90        100        110        120 
KAYKELRDKG YTLGTALKFG ADFRVYDIGV IPKKGKRSER EHSKWVLYPV SKDETFDFYE 

       130        140        150 
FASKNRVAHS TRKKLLLGIV SDKIEFIEVS WKKP 

« Hide

References

[1]"The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism."
Waters E., Hohn M.J., Ahel I., Graham D.E., Adams M.D., Barnstead M., Beeson K.Y., Bibbs L., Bolanos R., Keller M., Kretz K., Lin X., Mathur E., Ni J., Podar M., Richardson T., Sutton G.G., Simon M. expand/collapse author list , Soell D., Stetter K.O., Short J.M., Noorderwier M.
Proc. Natl. Acad. Sci. U.S.A. 100:12984-12988(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Kin4-M.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017199 Genomic DNA. Translation: AAR39058.1.
RefSeqNP_963497.1. NC_005213.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IEYX-ray2.11A1-154[»]
ProteinModelPortalQ74MP4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228908.NEQ205.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAR39058; AAR39058; NEQ205.
GeneID2654445.
KEGGneq:NEQ205.

Phylogenomic databases

eggNOGCOG1676.
HOGENOMHOG000107823.
KOK01170.
OMANMGYLRL.

Enzyme and pathway databases

BioCycNEQU228908:GJB6-219-MONOMER.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
HAMAPMF_01833. EndA_short.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFPIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsTIGR00324. endA. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ74MP4.

Entry information

Entry nameENDA_NANEQ
AccessionPrimary (citable) accession number: Q74MP4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references