Q74KY8 (GCP_LACJO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp

Alternative name(s):
t(6)A37 threonylcarbamoyladenosine biosynthesis protein

Gene names

Name:	gcp
Ordered Locus Names:	LJ_0436

Organism

Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]

Taxonomic identifier

257314 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine By similarity. HAMAP MF_01445
Subcellular location	Cytoplasm Potential HAMAP MF_01445.
Sequence similarities	Belongs to the KAE1 / YgjD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp HAMAP MF_01445		PRO_0000303395

Sequences

	Sequence		Length	Mass (Da)
	Q74KY8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5559894D1D9F168D	FASTA	348	37,765
	10 20 30 40 50 60 MTKKDVRILA FESSCDETST AVIKNGREIE SLIVATQIKS HQRFGGVVPE VASRHHIEVI 70 80 90 100 110 120 TQITKEALDK ANATWDDIDA IAVTYGPGLV GALLIGVSAA KAASMATGIP LIGVDHIMGH 130 140 150 160 170 180 IMAAQLKDEI EYPALALQVS GGHTEIVLMK DPIHFEIVGD TRDDAAGEAY DKIGRVLGVN 190 200 210 220 230 240 YPAGKTIDEW AHKGKDTFHF PRAMMEDDDY DFSFSGLKSA FINTCHHADQ IHEELDKYDL 250 260 270 280 290 300 AASFQASVVD VLSHKTIRAI KEYKPKTFIL GGGVAANHGL RDRLAEEIEK LPADIKPKVI 310 320 330 340 LPDLKLCGDN AAMIGAAAYN LYQAGKFSDV NLNADPSLEL PYADSMLK

References

[1]

"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533." Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A. Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed: 14983040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CNCM I-1225 / La1 / NCC 533.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017198 Genomic DNA. Translation: AAS08427.1.
RefSeq	NP_964461.1. NC_005362.1.
3D structure databases
ProteinModelPortal	Q74KY8.
ModBase	Search...
Protein family/group databases
MEROPS	M22.A01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2742153.
GenomeReviews	Gene locus LJ_0436 in contig AE017198_GR.
KEGG	ljo:LJ0436.
NMPDR	fig|257314.1.peg.447.
PATRIC	22237546. VBILacJoh1832_0462.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304663.
OMA	PAVGVHH.
ProtClustDB	PRK09604.
Enzyme and pathway databases
BioCyc	LJOH257314:LJ_0436-MONOMER.
Family and domain databases
HAMAP	MF_01445. gcp. [Tree]
InterPro	IPR022450. Pept_M22_O-sialoglycopept. IPR000905. Peptidase_M22. IPR017861. Peptidase_M22_subgr. [Graphical view]
KO	K01409.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR03723. Bact_gcp. 1 hit. TIGR00329. Gcp_kae1. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_LACJO

Accession

Primary (citable) accession number: Q74KY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program