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Q74KS1

- SYL_LACJO

UniProt

Q74KS1 - SYL_LACJO

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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei576 – 5761ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLJOH257314:GJN3-537-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:LJ_0680
OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Taxonomic identifieri257314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000581: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Leucine--tRNA ligasePRO_1000091329Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi257314.LJ0680.

Structurei

3D structure databases

ProteinModelPortaliQ74KS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012"HIGH" regionAdd
BLAST
Motifi573 – 5775"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiHISHELW.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q74KS1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYNHKTVEKK WQKYWAEHDT FKTGTDPKKK NYYALDMFPF PSGKGLHVGH
60 70 80 90 100
PEGYTATDIV SRMKRAQGYN VLHPMGWDAF GLPTEQYALK TGEDPEKVTK
110 120 130 140 150
ENIANFKKQL NKLGFSYDWD REVTTSDPNY YKWTQWVFEQ MYKKGLAYEA
160 170 180 190 200
EVPVNWSPDL GTVVANEEII DGKTERGGYP VYRRNMRQWM LKMTAYADRL
210 220 230 240 250
LEDLDDLDWP EPVKEMQRNW IGRSEGAQVT FKVKDSDKTF DVFTTRPDTL
260 270 280 290 300
FGVSYTVLAP ESKLVQEITT PEQKEAVDAY IKKIESKSDL ERTDLNKDKT
310 320 330 340 350
GVFTGAYAVN PVNGKEVPIW ISDYVLASYG TGAVMAVPAH DDRDYAFATK
360 370 380 390 400
FGLPINRVIE GGNLEKEAFG GDGKHINSEF LDGLNNEEAK KRMIEWLEDH
410 420 430 440 450
NVGEKKVNYK LRDWDFSRQR YWGEPIPVIH WEDGTTSLVP EDELPLRLPH
460 470 480 490 500
ATDIKPSGTP ESPLANLTDW VNVVDENGRK GKRETNTMPN WAGSSWYYLR
510 520 530 540 550
YIDPHNDKEL ADYDLLKKWL PVDLYIGGAE HAVRHLLYAR FWHKVLYDLG
560 570 580 590 600
VVPTKEPFQK LYNQGLILKN HEKMSKSKGN VVNPDEVIDE YGADSLRMYE
610 620 630 640 650
MFMGPLDASI DWDDNGPAST KKFLDRVWRL FVNDLDLKAI PQEKIVDEND
660 670 680 690 700
GELDKVYAET VKKVTEDFEA LHFNTAISQM MVFMNAAQKA KTIPREYAEG
710 720 730 740 750
FVQLLAPVAP HMMEEIWSVF GHDESIAYAK WPEYDPAKLV ESTVEIMVQV
760 770 780 790 800
NGKLRGKFKA AKDSDKDTLE KEALALDHVQ KFLEGKDVKK VIVIPNKIVN

IVAK
Length:804
Mass (Da):92,336
Last modified:July 5, 2004 - v1
Checksum:i8D7EAB45725646A9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017198 Genomic DNA. Translation: AAS08498.1.
RefSeqiNP_964532.1. NC_005362.1.

Genome annotation databases

EnsemblBacteriaiAAS08498; AAS08498; LJ_0680.
GeneIDi2742268.
KEGGiljo:LJ0680.
PATRICi22237702. VBILacJoh1832_0533.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017198 Genomic DNA. Translation: AAS08498.1 .
RefSeqi NP_964532.1. NC_005362.1.

3D structure databases

ProteinModelPortali Q74KS1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 257314.LJ0680.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS08498 ; AAS08498 ; LJ_0680 .
GeneIDi 2742268.
KEGGi ljo:LJ0680.
PATRICi 22237702. VBILacJoh1832_0533.

Phylogenomic databases

eggNOGi COG0495.
KOi K01869.
OMAi HISHELW.
OrthoDBi EOG63Z74X.

Enzyme and pathway databases

BioCyci LJOH257314:GJN3-537-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNCM I-1225 / La1 / NCC 533.

Entry informationi

Entry nameiSYL_LACJO
AccessioniPrimary (citable) accession number: Q74KS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3