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Q74KS1 (SYL_LACJO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LJ_0680
OrganismLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]
Taxonomic identifier257314 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091329

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif573 – 5775"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5761ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q74KS1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 8D7EAB45725646A9

FASTA80492,336
        10         20         30         40         50         60 
MYNHKTVEKK WQKYWAEHDT FKTGTDPKKK NYYALDMFPF PSGKGLHVGH PEGYTATDIV 

        70         80         90        100        110        120 
SRMKRAQGYN VLHPMGWDAF GLPTEQYALK TGEDPEKVTK ENIANFKKQL NKLGFSYDWD 

       130        140        150        160        170        180 
REVTTSDPNY YKWTQWVFEQ MYKKGLAYEA EVPVNWSPDL GTVVANEEII DGKTERGGYP 

       190        200        210        220        230        240 
VYRRNMRQWM LKMTAYADRL LEDLDDLDWP EPVKEMQRNW IGRSEGAQVT FKVKDSDKTF 

       250        260        270        280        290        300 
DVFTTRPDTL FGVSYTVLAP ESKLVQEITT PEQKEAVDAY IKKIESKSDL ERTDLNKDKT 

       310        320        330        340        350        360 
GVFTGAYAVN PVNGKEVPIW ISDYVLASYG TGAVMAVPAH DDRDYAFATK FGLPINRVIE 

       370        380        390        400        410        420 
GGNLEKEAFG GDGKHINSEF LDGLNNEEAK KRMIEWLEDH NVGEKKVNYK LRDWDFSRQR 

       430        440        450        460        470        480 
YWGEPIPVIH WEDGTTSLVP EDELPLRLPH ATDIKPSGTP ESPLANLTDW VNVVDENGRK 

       490        500        510        520        530        540 
GKRETNTMPN WAGSSWYYLR YIDPHNDKEL ADYDLLKKWL PVDLYIGGAE HAVRHLLYAR 

       550        560        570        580        590        600 
FWHKVLYDLG VVPTKEPFQK LYNQGLILKN HEKMSKSKGN VVNPDEVIDE YGADSLRMYE 

       610        620        630        640        650        660 
MFMGPLDASI DWDDNGPAST KKFLDRVWRL FVNDLDLKAI PQEKIVDEND GELDKVYAET 

       670        680        690        700        710        720 
VKKVTEDFEA LHFNTAISQM MVFMNAAQKA KTIPREYAEG FVQLLAPVAP HMMEEIWSVF 

       730        740        750        760        770        780 
GHDESIAYAK WPEYDPAKLV ESTVEIMVQV NGKLRGKFKA AKDSDKDTLE KEALALDHVQ 

       790        800 
KFLEGKDVKK VIVIPNKIVN IVAK 

« Hide

References

[1]"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533."
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CNCM I-1225 / La1 / NCC 533.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017198 Genomic DNA. Translation: AAS08498.1.
RefSeqNP_964532.1. NC_005362.1.

3D structure databases

ProteinModelPortalQ74KS1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257314.LJ0680.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS08498; AAS08498; LJ_0680.
GeneID2742268.
KEGGljo:LJ0680.
PATRIC22237702. VBILacJoh1832_0533.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycLJOH257314:GJN3-537-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LACJO
AccessionPrimary (citable) accession number: Q74KS1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries