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Q74KS1

- SYL_LACJO

UniProt

Q74KS1 - SYL_LACJO

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei576 – 5761ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciLJOH257314:GJN3-537-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:LJ_0680
    OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
    Taxonomic identifieri257314 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000000581: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 804804Leucine--tRNA ligasePRO_1000091329Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi257314.LJ0680.

    Structurei

    3D structure databases

    ProteinModelPortaliQ74KS1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi39 – 5012"HIGH" regionAdd
    BLAST
    Motifi573 – 5775"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    KOiK01869.
    OMAiHISHELW.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q74KS1-1 [UniParc]FASTAAdd to Basket

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    MYNHKTVEKK WQKYWAEHDT FKTGTDPKKK NYYALDMFPF PSGKGLHVGH    50
    PEGYTATDIV SRMKRAQGYN VLHPMGWDAF GLPTEQYALK TGEDPEKVTK 100
    ENIANFKKQL NKLGFSYDWD REVTTSDPNY YKWTQWVFEQ MYKKGLAYEA 150
    EVPVNWSPDL GTVVANEEII DGKTERGGYP VYRRNMRQWM LKMTAYADRL 200
    LEDLDDLDWP EPVKEMQRNW IGRSEGAQVT FKVKDSDKTF DVFTTRPDTL 250
    FGVSYTVLAP ESKLVQEITT PEQKEAVDAY IKKIESKSDL ERTDLNKDKT 300
    GVFTGAYAVN PVNGKEVPIW ISDYVLASYG TGAVMAVPAH DDRDYAFATK 350
    FGLPINRVIE GGNLEKEAFG GDGKHINSEF LDGLNNEEAK KRMIEWLEDH 400
    NVGEKKVNYK LRDWDFSRQR YWGEPIPVIH WEDGTTSLVP EDELPLRLPH 450
    ATDIKPSGTP ESPLANLTDW VNVVDENGRK GKRETNTMPN WAGSSWYYLR 500
    YIDPHNDKEL ADYDLLKKWL PVDLYIGGAE HAVRHLLYAR FWHKVLYDLG 550
    VVPTKEPFQK LYNQGLILKN HEKMSKSKGN VVNPDEVIDE YGADSLRMYE 600
    MFMGPLDASI DWDDNGPAST KKFLDRVWRL FVNDLDLKAI PQEKIVDEND 650
    GELDKVYAET VKKVTEDFEA LHFNTAISQM MVFMNAAQKA KTIPREYAEG 700
    FVQLLAPVAP HMMEEIWSVF GHDESIAYAK WPEYDPAKLV ESTVEIMVQV 750
    NGKLRGKFKA AKDSDKDTLE KEALALDHVQ KFLEGKDVKK VIVIPNKIVN 800
    IVAK 804
    Length:804
    Mass (Da):92,336
    Last modified:July 5, 2004 - v1
    Checksum:i8D7EAB45725646A9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017198 Genomic DNA. Translation: AAS08498.1.
    RefSeqiNP_964532.1. NC_005362.1.

    Genome annotation databases

    EnsemblBacteriaiAAS08498; AAS08498; LJ_0680.
    GeneIDi2742268.
    KEGGiljo:LJ0680.
    PATRICi22237702. VBILacJoh1832_0533.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017198 Genomic DNA. Translation: AAS08498.1 .
    RefSeqi NP_964532.1. NC_005362.1.

    3D structure databases

    ProteinModelPortali Q74KS1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257314.LJ0680.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAS08498 ; AAS08498 ; LJ_0680 .
    GeneIDi 2742268.
    KEGGi ljo:LJ0680.
    PATRICi 22237702. VBILacJoh1832_0533.

    Phylogenomic databases

    eggNOGi COG0495.
    KOi K01869.
    OMAi HISHELW.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci LJOH257314:GJN3-537-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CNCM I-1225 / La1 / NCC 533.

    Entry informationi

    Entry nameiSYL_LACJO
    AccessioniPrimary (citable) accession number: Q74KS1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3