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Q74KL4 (Q74KL4_LACJO) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-galactosidase PIRNR PIRNR001084
Short name=Beta-gal PIRNR PIRNR001084
EC=3.2.1.23 PIRNR PIRNR001084
Gene names
Ordered Locus Names:LJ_0738
OrganismLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]
Taxonomic identifier257314 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length678 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. PIRNR PIRNR001084

Sequence similarities

Belongs to the glycosyl hydrolase 42 family. PIRNR PIRNR001084

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1531Proton donor By similarity PIRSR PIRSR001084-1
Active site3161Nucleophile By similarity PIRSR PIRSR001084-1
Metal binding1181Zinc By similarity PIRSR PIRSR001084-3
Metal binding1581Zinc By similarity PIRSR PIRSR001084-3
Metal binding1601Zinc By similarity PIRSR PIRSR001084-3
Metal binding1631Zinc By similarity PIRSR PIRSR001084-3
Binding site1141Substrate By similarity PIRSR PIRSR001084-2
Binding site1521Substrate By similarity PIRSR PIRSR001084-2
Binding site3241Substrate By similarity PIRSR PIRSR001084-2

Sequences

Sequence LengthMass (Da)Tools
Q74KL4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: E6A94C3B1843B1A2

FASTA67878,941
        10         20         30         40         50         60 
MKRALDINQF LHGGDYNPEQ WWDEPDVINQ DFALFKQAKI NTVTVGIFSW AKLEPQEGNY 

        70         80         90        100        110        120 
DFSWLDEIFD RVEEMNGHVI LATPSGARPA WLAQKYPEVL RTDNRGNKRG FGGRHNHCLT 

       130        140        150        160        170        180 
SPIYRKKVYE INTKLAEHFG QRKSLVLWHI SNEYSGECYC DLCKDAFRKW LKNKYGDLAT 

       190        200        210        220        230        240 
LNHAWWNTFW SHTYNDWSQV NPPSPLSEMG NKGMNLDWKR FITDQTISFI DNETAPLKKI 

       250        260        270        280        290        300 
TPNIPVTTNM MAGNPLMDPF AGFDYQKVAR HSDFISWDSY PAWSNDSQST EELGRNIGLI 

       310        320        330        340        350        360 
HDFFRSLKHQ NFLVMENTPS RVNWHNFDRA KRPGMHELAS LQDVAHGSQG VLYFQLRASR 

       370        380        390        400        410        420 
GSSEMFHGAV IEHRHPEKTR AFKDVTKVGK DLEKISPIVA TNYAKAKVAI VFSYDSYWAL 

       430        440        450        460        470        480 
QDAESYSENK KVWQTIQKHY RYFYDHDIPV DFVSPEDEFS QYDLLIDPMH FLMSKSYLEK 

       490        500        510        520        530        540 
IDNYVKNGGK IVGTYISGVV DENDLAYMNE WPKELQEIYG LEPLETDVLY PRQSNLINFE 

       550        560        570        580        590        600 
GKEYPVHDYC ETLINCTGKV LATYSTDFYQ DTPAIVEHSY GAGKSYYLAC RTDYSFLEKF 

       610        620        630        640        650        660 
YEKIASELVP EIPITKFNDR VSIQIRENKS EKYWFVQNFS DKEEKIELSC ELVNLLDNTK 

       670 
ETGEVVLEPF ESKVYKNN

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References

[1]"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533."
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed: 14983040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CNCM I-1225 / La1 / NCC 533.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017198 Genomic DNA. Translation: AAS08556.1.
RefSeqNP_964590.1. NC_005362.1.

3D structure databases

ProteinModelPortalQ74KL4.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2743617.
GenomeReviewsGene locus LJ_0738 in contig AE017198_GR.
KEGGljo:LJ0738.
NMPDRfig|257314.1.peg.576.
PATRIC22237822. VBILacJoh1832_0593.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG476453.
OMAAKRPGMH.
ProtClustDBCLSK582025.

Family and domain databases

InterProIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KOK12308.
PfamPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFPIRSF001084. B-galactosidase. 1 hit.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ74KL4_LACJO
AccessionPrimary (citable) accession number: Q74KL4
Entry history
Integrated into UniProtKB/TrEMBL: July 5, 2004
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info