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Q74KC7 (NADK_LACJO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:LJ_0824
OrganismLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]
Taxonomic identifier257314 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 270270NAD kinase HAMAP-Rule MF_00361
PRO_0000229647

Regions

Nucleotide binding45 – 462NAD By similarity
Nucleotide binding121 – 1222NAD By similarity
Nucleotide binding160 – 1656NAD By similarity

Sites

Active site451Proton acceptor By similarity
Binding site1471NAD By similarity
Binding site1491NAD By similarity
Binding site1841NAD; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q74KC7 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: D053A8CF33A79178

FASTA27030,637
        10         20         30         40         50         60 
MKVALVYNDK VETLAVVKAL EKLLNARKIE IDPENPDVVI TIGGDGTLIS GFHKYQNLVD 

        70         80         90        100        110        120 
KIRFIGVHTG HLGFYTDWRN FEINKMVDNL TKKQPSSASY PLLELIITTG AGEKKKLLAL 

       130        140        150        160        170        180 
NEATIKRVSK TLKADVYIRD QFFESFKGDG LCVSTPTGST AYSKSLGGAV IHPRLKALQM 

       190        200        210        220        230        240 
TEIASINNRV FRTLSSPIVI APDEWITIKP ETGSDDHYVV TFDGYEFNHK HIKKIEYRIS 

       250        260        270 
QHVIRFDKYQ HTHFWNRVED AFIGQPKDKQ 

« Hide

References

[1]"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533."
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CNCM I-1225 / La1 / NCC 533.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017198 Genomic DNA. Translation: AAS08645.1.
RefSeqNP_964679.1. NC_005362.1.

3D structure databases

ProteinModelPortalQ74KC7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257314.LJ0824.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS08645; AAS08645; LJ_0824.
GeneID2742061.
KEGGljo:LJ0824.
PATRIC22238000. VBILacJoh1832_0681.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
KOK00858.
OMAIQMSEIA.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycLJOH257314:GJN3-685-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_LACJO
AccessionPrimary (citable) accession number: Q74KC7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families