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Q74K95 (Q74K95_LACJO) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-galactosidase PIRNR PIRNR001084
Short name=Beta-gal PIRNR PIRNR001084
EC=3.2.1.23 PIRNR PIRNR001084
Gene names
Ordered Locus Names:LJ_0858
OrganismLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]
Taxonomic identifier257314 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length668 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. PIRNR PIRNR001084

Sequence similarities

Belongs to the glycosyl hydrolase 42 family. PIRNR PIRNR001084

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1481Proton donor By similarity PIRSR PIRSR001084-1
Active site3071Nucleophile By similarity PIRSR PIRSR001084-1
Metal binding1131Zinc By similarity PIRSR PIRSR001084-3
Metal binding1531Zinc By similarity PIRSR PIRSR001084-3
Metal binding1551Zinc By similarity PIRSR PIRSR001084-3
Metal binding1581Zinc By similarity PIRSR PIRSR001084-3
Binding site1091Substrate By similarity PIRSR PIRSR001084-2
Binding site1471Substrate By similarity PIRSR PIRSR001084-2
Binding site3151Substrate By similarity PIRSR PIRSR001084-2

Sequences

Sequence LengthMass (Da)Tools
Q74K95 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 9672F78829C18DCD

FASTA66876,689
        10         20         30         40         50         60 
MTQLSRFLYG GDYNPEQWPE DTWANDIQVF KQAHLNSATI NVFSWALLEQ REGEYDFSKL 

        70         80         90        100        110        120 
DKIVKELSDA NFDIVLATAT AAMPAWMFKK YPDVARVDYQ GRRHVFGQRH NFCSNSKNYQ 

       130        140        150        160        170        180 
VLASKLVKKI VSRYYKNSHI KVWHVNNEYG GNCYCDNCQN AFRDWLKYKY KNLETLNKAW 

       190        200        210        220        230        240 
NMNVWGHRIY DWDEIVVPNE LGDAWGAEGT ETIVSALSLD YLRFQSESLL KLFKMEKAII 

       250        260        270        280        290        300 
KKYDPVALVT TNFHALPNKL LDYQKWAKEQ DIISYDSYPS YNMPTYQSAF LYDLMRSLKH 

       310        320        330        340        350        360 
QPFMLMESTP AQVNWQPYSP LKRPGQMAAT ELQAVAHGAD TVQFFQLKQA IGGSEKFHSA 

       370        380        390        400        410        420 
VISHSQRTDT RVFKEVTELG EKLEKTGSTI LGSKTKAKVG IVFDWNNFWS YEYIDGITQD 

       430        440        450        460        470        480 
LDYVESILDY YKQFYKRNIP TDIISVDDDF SQYDLVVAPV LYMIKSGLAE KIDQYVKKGG 

       490        500        510        520        530        540 
NFVASYLSGI VNENDSVYLG GYPGPLKDVL GIWVEESDAV IPGQKITVSL DSNNYQANLI 

       550        560        570        580        590        600 
CDLLHLEGAH ALGNYNSEFY KETPAVSENQ WGKGTSWYIG TQLDEAGLSK IFDHLISIVN 

       610        620        630        640        650        660 
IKSLIETKTD LEITKRVTKS GKELYFVLNM SNDTKDLPSE FDAYKNLFTD KSANKKMKAW 


EVQLLSKL

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References

[1]"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533."
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed: 14983040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CNCM I-1225 / La1 / NCC 533.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017198 Genomic DNA. Translation: AAS08679.1.
RefSeqNP_964713.1. NC_005362.1.

3D structure databases

ProteinModelPortalQ74K95.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2743501.
GenomeReviewsGene locus LJ_0858 in contig AE017198_GR.
KEGGljo:LJ0858.
NMPDRfig|257314.1.peg.699.
PATRIC22238068. VBILacJoh1832_0715.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG476453.
OMAKVTPDIP.
ProtClustDBCLSK829419.

Family and domain databases

InterProIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KOK01190.
PfamPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFPIRSF001084. B-galactosidase. 1 hit.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ74K95_LACJO
AccessionPrimary (citable) accession number: Q74K95
Entry history
Integrated into UniProtKB/TrEMBL: July 5, 2004
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info