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Protein

Enolase 1

Gene

eno1

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 1 (gpmA1), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 2 (gpmA2)
  4. Enolase 2 (eno2), Enolase 1 (eno1), Enolase 3 (eno3)
  5. Pyruvate kinase (LJ_1080)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155SubstrateUniRule annotation1
Binding sitei164SubstrateUniRule annotation1
Active sitei205Proton donorUniRule annotation1
Metal bindingi242MagnesiumUniRule annotation1
Metal bindingi287MagnesiumUniRule annotation1
Binding sitei287SubstrateUniRule annotation1
Metal bindingi314MagnesiumUniRule annotation1
Binding sitei314SubstrateUniRule annotation1
Active sitei339Proton acceptorUniRule annotation1
Binding sitei339Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei390SubstrateUniRule annotation1

GO - Molecular functioni

  • laminin binding Source: CAFA
  • magnesium ion binding Source: InterPro
  • phosphopyruvate hydratase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Protein family/group databases

MoonProtiQ74K78

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 1UniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 1UniRule annotation
2-phosphoglycerate dehydratase 1UniRule annotation
Gene namesi
Name:eno1UniRule annotation
Ordered Locus Names:LJ_0875
OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Taxonomic identifieri257314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000000581 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001339011 – 432Enolase 1Add BLAST432

Proteomic databases

PRIDEiQ74K78

Interactioni

GO - Molecular functioni

  • laminin binding Source: CAFA

Protein-protein interaction databases

STRINGi257314.LJ0875

Structurei

3D structure databases

ProteinModelPortaliQ74K78
SMRiQ74K78
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni366 – 369Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C70 Bacteria
COG0148 LUCA
KOiK01689
OMAiEFMIIPV

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q74K78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVITDIHAR EVLDSRGNPT VEAEVYTELG GFGRAIVPSG ASTGEHEAVE
60 70 80 90 100
LRDGDKSRFL GQGVLTAVEN VNGEIAKAVI GLDVTDQRLI DQTMIDLDGT
110 120 130 140 150
PNKGRLGANA MLAVSLASAR AAADELGLPL YEYLGGPNAH VLPTPMMNVI
160 170 180 190 200
NGGKHADNNV DIQEFMIMPV GAKSLHEAVR MGAETFHTLK SLLQERGEST
210 220 230 240 250
AVGDEGGFAP NLKNNEEPFE ILVEAIQRAG YKPGQDISIA FDCAASEFYN
260 270 280 290 300
KDTKKYVTVA DGREYTAEEW TSLMEDIVDK YPVISIEDPL DENDWEGWKV
310 320 330 340 350
FTERLGDKVQ IVGDDLFVTN TNYLEKGIKM GVANSILIKL NQIGTLTETF
360 370 380 390 400
EAIEMAKEAG YTAVVSHRSG ETEDTTIADL VVATNAGQIK TGSMSRTDRI
410 420 430
AKYNQLMRIE EALGSTAQYK GIHSFYNLHK QF
Length:432
Mass (Da):47,072
Last modified:July 5, 2004 - v1
Checksum:i48F322BE095258C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017198 Genomic DNA Translation: AAS08696.1
RefSeqiWP_004897701.1, NC_005362.1

Genome annotation databases

EnsemblBacteriaiAAS08696; AAS08696; LJ_0875
KEGGiljo:LJ_0875

Similar proteinsi

Entry informationi

Entry nameiENO1_LACJO
AccessioniPrimary (citable) accession number: Q74K78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health