Q74K70 (UNG_LACJO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uracil-DNA glycosylase
Short name=UDG
EC=3.2.2.27

Gene names

Name:	ung
Ordered Locus Names:	LJ_0883

Organism

Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]

Taxonomic identifier

257314 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus johnsonii

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine By similarity. HAMAP-Rule MF_00148
Catalytic activity	Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil. HAMAP-Rule MF_00148
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the uracil-DNA glycosylase family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	uracil DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Uracil-DNA glycosylase HAMAP-Rule MF_00148		PRO_0000176106
Sites
	Active site	65	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q74K70 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4795E80B14218D46	FASTA	231	25,886
	10 20 30 40 50 60 MKKLIGNDWD EVLAPAFESE KYQELRDFLK EEYQTKQIFP DMYHIFTAFK LTSFADTKVV 70 80 90 100 110 120 ILGQDPYHNP GQATGMSFSV NPGVPLPPSL KNIYKELYDD VGAVPVNHGY LKKWADQGVL 130 140 150 160 170 180 LLNAVLTVPY GNANGHRGKG WELITDTAIK ALSDRGKVVF ILWGKFAQNK IPLIDEGKNV 190 200 210 220 230 IIKSPHPSPF SADRGFFGSR PFSRCNDALI KFGESPIDWQ LPQEVNPADM A

References

[1]

"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533." Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A. Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CNCM I-1225 / La1 / NCC 533.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017198 Genomic DNA. Translation: AAS08704.1.
RefSeq	NP_964738.1. NC_005362.1.
3D structure databases
ProteinModelPortal	Q74K70.
SMR	Q74K70. Positions 1-226.
ModBase	Search...
Protein-protein interaction databases
STRING	257314.LJ0883.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS08704; AAS08704; LJ_0883.
GeneID	2743712.
KEGG	ljo:LJ0883.
PATRIC	22238120. VBILacJoh1832_0740.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0692.
KO	K03648.
OMA	AGKEIYP.
ProtClustDB	PRK05254.
Enzyme and pathway databases
BioCyc	LJOH257314:GJN3-745-MONOMER.
Family and domain databases
Gene3D	3.40.470.10. 1 hit.
HAMAP	MF_00148. UDG.
InterPro	IPR018085. Ura-DNA_Glyclase_AS. IPR002043. Ura_DNA_glycsylse. IPR005122. Uracil-DNA_glycosylase-like. [Graphical view]
PANTHER	PTHR11264. PTHR11264. 1 hit.
Pfam	PF03167. UDG. 1 hit. [Graphical view]
SMART	SM00986. UDG. 1 hit. [Graphical view]
SUPFAM	SSF52141. UDNA_glycsylseSF. 1 hit.
TIGRFAMs	TIGR00628. ung. 1 hit.
PROSITE	PS00130. U_DNA_GLYCOSYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UNG_LACJO

Accession

Primary (citable) accession number: Q74K70

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program