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Q74K65 (POTA_LACJO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Spermidine/putrescine import ATP-binding protein PotA

EC=3.6.3.31
Gene names
Name:potA
Ordered Locus Names:LJ_0888
OrganismLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]
Taxonomic identifier257314 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system By similarity.

Catalytic activity

ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).

Subunit structure

The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD) Potential.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification]

Contains 1 ABC transporter domain.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentATP-binding cassette (ABC) transporter complex

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

putrescine-importing ATPase activity

Inferred from electronic annotation. Source: InterPro

spermidine-importing ATPase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 363363Spermidine/putrescine import ATP-binding protein PotA
PRO_0000286232

Regions

Domain5 – 236232ABC transporter
Nucleotide binding38 – 458ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q74K65 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: B7B6DBDE6EB9B113

FASTA36341,103
        10         20         30         40         50         60 
MSDIIKLKHV RKEYDDGFVA LKDINLTIES GKFYSLLGPS GSGKTTILRI IAGFSEPTSG 

        70         80         90        100        110        120 
QVFFDGQDIT NLDAAKRKIN TVFQNYALFP HMNVFENVAF GLQIKKKDKQ EIKLAVKEAL 

       130        140        150        160        170        180 
HMVQLDGFAN REISELSGGQ QQRVAIARAI VNQPKVLLLD ESLSALDKRL RKDMQFELRE 

       190        200        210        220        230        240 
IQKKLGITFI FVTHDQEEAL AMSDEIFVLN EGKIQQSGSP VDIYDEPVND FVARFIGDSN 

       250        260        270        280        290        300 
ILSGRMIKDY EVEFGNHRFE CADAGIKPGE KVEVVLRPED LDITDIEHGK LRVVVESQLF 

       310        320        330        340        350        360 
LGDHFEIKAI DSDENEWLIH STNPTKIGKE VGVYFDPEDI HVMRFGESEA EFDKRLEAYE 


GEE 

« Hide

References

[1]"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533."
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CNCM I-1225 / La1 / NCC 533.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017198 Genomic DNA. Translation: AAS08709.1.
RefSeqNP_964743.1. NC_005362.1.

3D structure databases

ProteinModelPortalQ74K65.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257314.LJ0888.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS08709; AAS08709; LJ_0888.
GeneID2742720.
KEGGljo:LJ0888.
PATRIC22238130. VBILacJoh1832_0745.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3842.
KOK11072.
OMAYDEPVND.
OrthoDBEOG6T7N3V.

Enzyme and pathway databases

BioCycLJOH257314:GJN3-750-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR017879. ABC_Sperm/Put_ATP-bd_PotA.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR005893. Sp_pt_ABC_ATP-bd.
IPR013611. Transp-assoc_OB_typ2.
[Graphical view]
PfamPF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR01187. potA. 1 hit.
PROSITEPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51305. POTA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePOTA_LACJO
AccessionPrimary (citable) accession number: Q74K65
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families