Q74K65 (POTA_LACJO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine/putrescine import ATP-binding protein PotA
EC=3.6.3.31

Gene names

Name:	potA
Ordered Locus Names:	LJ_0888

Organism

Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]

Taxonomic identifier

257314 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus johnsonii

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system By similarity.
Catalytic activity	ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).
Subunit structure	The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD) Potential.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	ATP-binding cassette (ABC) transporter complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW putrescine-importing ATPase activity Inferred from electronic annotation. Source: InterPro spermidine-importing ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Spermidine/putrescine import ATP-binding protein PotA		PRO_0000286232
Regions
	Domain	5 – 236	232	ABC transporter
	Nucleotide binding	38 – 45	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q74K65 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B7B6DBDE6EB9B113	FASTA	363	41,103
	10 20 30 40 50 60 MSDIIKLKHV RKEYDDGFVA LKDINLTIES GKFYSLLGPS GSGKTTILRI IAGFSEPTSG 70 80 90 100 110 120 QVFFDGQDIT NLDAAKRKIN TVFQNYALFP HMNVFENVAF GLQIKKKDKQ EIKLAVKEAL 130 140 150 160 170 180 HMVQLDGFAN REISELSGGQ QQRVAIARAI VNQPKVLLLD ESLSALDKRL RKDMQFELRE 190 200 210 220 230 240 IQKKLGITFI FVTHDQEEAL AMSDEIFVLN EGKIQQSGSP VDIYDEPVND FVARFIGDSN 250 260 270 280 290 300 ILSGRMIKDY EVEFGNHRFE CADAGIKPGE KVEVVLRPED LDITDIEHGK LRVVVESQLF 310 320 330 340 350 360 LGDHFEIKAI DSDENEWLIH STNPTKIGKE VGVYFDPEDI HVMRFGESEA EFDKRLEAYE GEE

References

[1]

"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533." Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A. Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CNCM I-1225 / La1 / NCC 533.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017198 Genomic DNA. Translation: AAS08709.1.
RefSeq	NP_964743.1. NC_005362.1.
3D structure databases
ProteinModelPortal	Q74K65.
ModBase	Search...
Protein-protein interaction databases
STRING	257314.LJ0888.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS08709; AAS08709; LJ_0888.
GeneID	2742720.
KEGG	ljo:LJ0888.
PATRIC	22238130. VBILacJoh1832_0745.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3842.
KO	K11072.
OMA	ERHEENS.
ProtClustDB	CLSK925162.
Enzyme and pathway databases
BioCyc	LJOH257314:GJN3-750-MONOMER.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR017879. ABC_Sperm/Put_ATP-bd_PotA. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR008995. Mo/tungstate-bd_C_term_dom. IPR027417. P-loop_NTPase. IPR005893. Sp_pt_ABC_ATP-bd. IPR013611. Transp-assoc_OB_typ2. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. PF08402. TOBE_2. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF50331. MOP_like. 1 hit. SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR01187. potA. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51305. POTA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POTA_LACJO

Accession

Primary (citable) accession number: Q74K65

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program