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Q74JX6

- SYI_LACJO

UniProt

Q74JX6 - SYI_LACJO

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation
Metal bindingi887 – 8871ZincUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLJOH257314:GJN3-844-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LJ_0980
OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Taxonomic identifieri257314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000581: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 928928Isoleucine--tRNA ligasePRO_0000098402Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi257314.LJ0980.

Structurei

3D structure databases

ProteinModelPortaliQ74JX6.
SMRiQ74JX6. Positions 1-916.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q74JX6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVKDTLNLG KTKFKMRGNL PVREAEWQKE WEENKLYEQR LKLNEGKPRF
60 70 80 90 100
DLHDGPPFAN GNIHMGHSLN KISKDIIVRF KNMNGYYAPY VPGWDTHGLP
110 120 130 140 150
VEQQLAKKGV DRKTMDRAKY RELCRQFAEE QVQKQMADFK RLGVMADWDH
160 170 180 190 200
PYITLQPKFE AEEIRVFGEM FKKGYIYKGK KPVYWSWSSE STLAEAEVEY
210 220 230 240 250
HDIKSPRIYV AFPIKDGKGI LDSDTSLVIW TTTPWTIPSN VGITVNPKFD
260 270 280 290 300
YSVVEVNGKK YVIGSQRLSA VAEDLGWEDY KVVKTLKGTD FDRMTYQHPL
310 320 330 340 350
YDVTGVIMND TYVTADDGTG LVHNATGFGE DDYNVGRRYG LPVFSPMDAQ
360 370 380 390 400
GRFTKEVPDP DLVGMFYDDA NKVVADKLEK AGALLKLSFF THSYPHDWRT
410 420 430 440 450
KKPVIYRATT QWFASIDKFR DQILAEIEKA NFIPAWGKTR LYNMIKDRGD
460 470 480 490 500
WVISRQRAWG VPLPIFYAED DTPIVTPETI EHVAQIFEKE GSNAWYTYTA
510 520 530 540 550
EELLPEGFKS EHSPNGKFRK ETDILDVWFD SGSSWAGVMQ ERDGLGFPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSLITS VAVTGKAPYK QVLSQGFVLD DKGHKMSKSL
610 620 630 640 650
GNVISPNDVI KQMGAEIIRL WVAGADTTSD VAVSQDILRQ SAESYRKIRN
660 670 680 690 700
TMRFMLANTS DFDPKEDAIA YPDMSGVDQY MEIKLNRLIE EAIEAYNKFD
710 720 730 740 750
FNSVYKKVFS FISNDLSAFY LDFAKDILYI DAEDSETRRS MQTVIYDVLV
760 770 780 790 800
KLTKLMTPIL PHTMEEVWGY LKEPEDYVQL ANMPEVDHFA NEDEVLADWN
810 820 830 840 850
AFMKVRSDVL KALEKARNAK VIGKSFEAHV TLYPTEETKA LLDKLNANIR
860 870 880 890 900
QILIVSDLTI SDEEAPENAE KLPTASIVVE HAAGEVCPRC RRTTTDVGSD
910 920
PRFPELCARC AAIVAENFPE AEKEGLEK
Length:928
Mass (Da):105,960
Last modified:December 20, 2005 - v2
Checksum:i4A2477DC031533A6
GO

Sequence cautioni

The sequence AAS08801.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017198 Genomic DNA. Translation: AAS08801.1. Different initiation.
RefSeqiNP_964835.1. NC_005362.1.

Genome annotation databases

EnsemblBacteriaiAAS08801; AAS08801; LJ_0980.
GeneIDi2742127.
KEGGiljo:LJ0980.
PATRICi22238322. VBILacJoh1832_0839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017198 Genomic DNA. Translation: AAS08801.1 . Different initiation.
RefSeqi NP_964835.1. NC_005362.1.

3D structure databases

ProteinModelPortali Q74JX6.
SMRi Q74JX6. Positions 1-916.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 257314.LJ0980.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS08801 ; AAS08801 ; LJ_0980 .
GeneIDi 2742127.
KEGGi ljo:LJ0980.
PATRICi 22238322. VBILacJoh1832_0839.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LJOH257314:GJN3-844-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNCM I-1225 / La1 / NCC 533.

Entry informationi

Entry nameiSYI_LACJO
AccessioniPrimary (citable) accession number: Q74JX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3