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Q74JX6

- SYI_LACJO

UniProt

Q74JX6 - SYI_LACJO

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
    Binding sitei598 – 5981ATPUniRule annotation
    Metal bindingi887 – 8871ZincUniRule annotation
    Metal bindingi890 – 8901ZincUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLJOH257314:GJN3-844-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:LJ_0980
    OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
    Taxonomic identifieri257314 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000000581: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 928928Isoleucine--tRNA ligasePRO_0000098402Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi257314.LJ0980.

    Structurei

    3D structure databases

    ProteinModelPortaliQ74JX6.
    SMRiQ74JX6. Positions 1-916.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi595 – 5995"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q74JX6-1 [UniParc]FASTAAdd to Basket

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    MRVKDTLNLG KTKFKMRGNL PVREAEWQKE WEENKLYEQR LKLNEGKPRF    50
    DLHDGPPFAN GNIHMGHSLN KISKDIIVRF KNMNGYYAPY VPGWDTHGLP 100
    VEQQLAKKGV DRKTMDRAKY RELCRQFAEE QVQKQMADFK RLGVMADWDH 150
    PYITLQPKFE AEEIRVFGEM FKKGYIYKGK KPVYWSWSSE STLAEAEVEY 200
    HDIKSPRIYV AFPIKDGKGI LDSDTSLVIW TTTPWTIPSN VGITVNPKFD 250
    YSVVEVNGKK YVIGSQRLSA VAEDLGWEDY KVVKTLKGTD FDRMTYQHPL 300
    YDVTGVIMND TYVTADDGTG LVHNATGFGE DDYNVGRRYG LPVFSPMDAQ 350
    GRFTKEVPDP DLVGMFYDDA NKVVADKLEK AGALLKLSFF THSYPHDWRT 400
    KKPVIYRATT QWFASIDKFR DQILAEIEKA NFIPAWGKTR LYNMIKDRGD 450
    WVISRQRAWG VPLPIFYAED DTPIVTPETI EHVAQIFEKE GSNAWYTYTA 500
    EELLPEGFKS EHSPNGKFRK ETDILDVWFD SGSSWAGVMQ ERDGLGFPAD 550
    LYLEGSDQYR GWFNSSLITS VAVTGKAPYK QVLSQGFVLD DKGHKMSKSL 600
    GNVISPNDVI KQMGAEIIRL WVAGADTTSD VAVSQDILRQ SAESYRKIRN 650
    TMRFMLANTS DFDPKEDAIA YPDMSGVDQY MEIKLNRLIE EAIEAYNKFD 700
    FNSVYKKVFS FISNDLSAFY LDFAKDILYI DAEDSETRRS MQTVIYDVLV 750
    KLTKLMTPIL PHTMEEVWGY LKEPEDYVQL ANMPEVDHFA NEDEVLADWN 800
    AFMKVRSDVL KALEKARNAK VIGKSFEAHV TLYPTEETKA LLDKLNANIR 850
    QILIVSDLTI SDEEAPENAE KLPTASIVVE HAAGEVCPRC RRTTTDVGSD 900
    PRFPELCARC AAIVAENFPE AEKEGLEK 928
    Length:928
    Mass (Da):105,960
    Last modified:December 20, 2005 - v2
    Checksum:i4A2477DC031533A6
    GO

    Sequence cautioni

    The sequence AAS08801.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017198 Genomic DNA. Translation: AAS08801.1. Different initiation.
    RefSeqiNP_964835.1. NC_005362.1.

    Genome annotation databases

    EnsemblBacteriaiAAS08801; AAS08801; LJ_0980.
    GeneIDi2742127.
    KEGGiljo:LJ0980.
    PATRICi22238322. VBILacJoh1832_0839.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017198 Genomic DNA. Translation: AAS08801.1 . Different initiation.
    RefSeqi NP_964835.1. NC_005362.1.

    3D structure databases

    ProteinModelPortali Q74JX6.
    SMRi Q74JX6. Positions 1-916.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257314.LJ0980.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAS08801 ; AAS08801 ; LJ_0980 .
    GeneIDi 2742127.
    KEGGi ljo:LJ0980.
    PATRICi 22238322. VBILacJoh1832_0839.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LJOH257314:GJN3-844-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CNCM I-1225 / La1 / NCC 533.

    Entry informationi

    Entry nameiSYI_LACJO
    AccessioniPrimary (citable) accession number: Q74JX6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 73 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3