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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei554Aminoacyl-adenylateUniRule annotation1
Binding sitei598ATPUniRule annotation1
Metal bindingi887ZincUniRule annotation1
Metal bindingi890ZincUniRule annotation1
Metal bindingi907ZincUniRule annotation1
Metal bindingi910ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLJOH257314:G1G0M-846-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LJ_0980
OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Taxonomic identifieri257314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000000581 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984021 – 928Isoleucine--tRNA ligaseAdd BLAST928

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi257314.LJ0980

Structurei

3D structure databases

ProteinModelPortaliQ74JX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi595 – 599"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q74JX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVKDTLNLG KTKFKMRGNL PVREAEWQKE WEENKLYEQR LKLNEGKPRF
60 70 80 90 100
DLHDGPPFAN GNIHMGHSLN KISKDIIVRF KNMNGYYAPY VPGWDTHGLP
110 120 130 140 150
VEQQLAKKGV DRKTMDRAKY RELCRQFAEE QVQKQMADFK RLGVMADWDH
160 170 180 190 200
PYITLQPKFE AEEIRVFGEM FKKGYIYKGK KPVYWSWSSE STLAEAEVEY
210 220 230 240 250
HDIKSPRIYV AFPIKDGKGI LDSDTSLVIW TTTPWTIPSN VGITVNPKFD
260 270 280 290 300
YSVVEVNGKK YVIGSQRLSA VAEDLGWEDY KVVKTLKGTD FDRMTYQHPL
310 320 330 340 350
YDVTGVIMND TYVTADDGTG LVHNATGFGE DDYNVGRRYG LPVFSPMDAQ
360 370 380 390 400
GRFTKEVPDP DLVGMFYDDA NKVVADKLEK AGALLKLSFF THSYPHDWRT
410 420 430 440 450
KKPVIYRATT QWFASIDKFR DQILAEIEKA NFIPAWGKTR LYNMIKDRGD
460 470 480 490 500
WVISRQRAWG VPLPIFYAED DTPIVTPETI EHVAQIFEKE GSNAWYTYTA
510 520 530 540 550
EELLPEGFKS EHSPNGKFRK ETDILDVWFD SGSSWAGVMQ ERDGLGFPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSLITS VAVTGKAPYK QVLSQGFVLD DKGHKMSKSL
610 620 630 640 650
GNVISPNDVI KQMGAEIIRL WVAGADTTSD VAVSQDILRQ SAESYRKIRN
660 670 680 690 700
TMRFMLANTS DFDPKEDAIA YPDMSGVDQY MEIKLNRLIE EAIEAYNKFD
710 720 730 740 750
FNSVYKKVFS FISNDLSAFY LDFAKDILYI DAEDSETRRS MQTVIYDVLV
760 770 780 790 800
KLTKLMTPIL PHTMEEVWGY LKEPEDYVQL ANMPEVDHFA NEDEVLADWN
810 820 830 840 850
AFMKVRSDVL KALEKARNAK VIGKSFEAHV TLYPTEETKA LLDKLNANIR
860 870 880 890 900
QILIVSDLTI SDEEAPENAE KLPTASIVVE HAAGEVCPRC RRTTTDVGSD
910 920
PRFPELCARC AAIVAENFPE AEKEGLEK
Length:928
Mass (Da):105,960
Last modified:December 20, 2005 - v2
Checksum:i4A2477DC031533A6
GO

Sequence cautioni

The sequence AAS08801 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017198 Genomic DNA Translation: AAS08801.1 Different initiation.
RefSeqiWP_044496687.1, NC_005362.1

Genome annotation databases

EnsemblBacteriaiAAS08801; AAS08801; LJ_0980
KEGGiljo:LJ_0980
PATRICifig|257314.6.peg.839

Similar proteinsi

Entry informationi

Entry nameiSYI_LACJO
AccessioniPrimary (citable) accession number: Q74JX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health