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Q74JW2

- DEF_LACJO

UniProt

Q74JW2 - DEF_LACJO

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Protein

Peptide deformylase

Gene

def

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

Cofactori

Binds 1 Fe2+ ion.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi111 – 1111IronUniRule annotation
Metal bindingi154 – 1541IronUniRule annotation
Active sitei155 – 1551UniRule annotation
Metal bindingi158 – 1581IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciLJOH257314:GJN3-858-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylaseUniRule annotation (EC:3.5.1.88UniRule annotation)
Short name:
PDFUniRule annotation
Alternative name(s):
Polypeptide deformylaseUniRule annotation
Gene namesi
Name:defUniRule annotation
Ordered Locus Names:LJ_0993
OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Taxonomic identifieri257314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000581: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 184184Peptide deformylasePRO_0000301047Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi257314.LJ0993.

Structurei

3D structure databases

ProteinModelPortaliQ74JW2.
SMRiQ74JW2. Positions 1-183.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0242.
KOiK01462.
OMAiSQDPKIA.
OrthoDBiEOG6PZXGQ.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

Sequencei

Sequence statusi: Complete.

Q74JW2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MILMHDITRD GNPVLRQVAK PLTFPLSDEY KKLADDMMQY LINSQDPKIA
60 70 80 90 100
EKHQLRAGVG LAAPQVGESV QMAALLVPND KGEIIFKEVF VNPKILSESV
110 120 130 140 150
RRTCLAEGEG CLSVDKDIEG YVPRPDKLKI RYYTVDGEEK TIRLKDYPAI
160 170 180
VASHEIDHLN GHLFYDRINK QNPFELAEDT IVIS
Length:184
Mass (Da):20,791
Last modified:July 5, 2004 - v1
Checksum:i43DDA10729328290
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017198 Genomic DNA. Translation: AAS08815.1.
RefSeqiNP_964849.1. NC_005362.1.

Genome annotation databases

EnsemblBacteriaiAAS08815; AAS08815; LJ_0993.
GeneIDi2743557.
KEGGiljo:LJ0993.
PATRICi22238350. VBILacJoh1832_0853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017198 Genomic DNA. Translation: AAS08815.1 .
RefSeqi NP_964849.1. NC_005362.1.

3D structure databases

ProteinModelPortali Q74JW2.
SMRi Q74JW2. Positions 1-183.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 257314.LJ0993.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS08815 ; AAS08815 ; LJ_0993 .
GeneIDi 2743557.
KEGGi ljo:LJ0993.
PATRICi 22238350. VBILacJoh1832_0853.

Phylogenomic databases

eggNOGi COG0242.
KOi K01462.
OMAi SQDPKIA.
OrthoDBi EOG6PZXGQ.

Enzyme and pathway databases

BioCyci LJOH257314:GJN3-858-MONOMER.

Family and domain databases

Gene3Di 3.90.45.10. 1 hit.
HAMAPi MF_00163. Pep_deformylase.
InterProi IPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view ]
PANTHERi PTHR10458. PTHR10458. 1 hit.
Pfami PF01327. Pep_deformylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF004749. Pep_def. 1 hit.
PRINTSi PR01576. PDEFORMYLASE.
SUPFAMi SSF56420. SSF56420. 1 hit.
TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNCM I-1225 / La1 / NCC 533.

Entry informationi

Entry nameiDEF_LACJO
AccessioniPrimary (citable) accession number: Q74JW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 5, 2004
Last modified: October 1, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3