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Q74JW2 (DEF_LACJO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide deformylase

Short name=PDF
EC=3.5.1.88
Alternative name(s):
Polypeptide deformylase
Gene names
Name:def
Ordered Locus Names:LJ_0993
OrganismLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]
Taxonomic identifier257314 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length184 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP-Rule MF_00163

Catalytic activity

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP-Rule MF_00163

Cofactor

Binds 1 Fe2+ ion By similarity. HAMAP-Rule MF_00163

Sequence similarities

Belongs to the polypeptide deformylase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandIron
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functioniron ion binding

Inferred from electronic annotation. Source: InterPro

peptide deformylase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 184184Peptide deformylase HAMAP-Rule MF_00163
PRO_0000301047

Sites

Active site1551 By similarity
Metal binding1111Iron By similarity
Metal binding1541Iron By similarity
Metal binding1581Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q74JW2 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 43DDA10729328290

FASTA18420,791
        10         20         30         40         50         60 
MILMHDITRD GNPVLRQVAK PLTFPLSDEY KKLADDMMQY LINSQDPKIA EKHQLRAGVG 

        70         80         90        100        110        120 
LAAPQVGESV QMAALLVPND KGEIIFKEVF VNPKILSESV RRTCLAEGEG CLSVDKDIEG 

       130        140        150        160        170        180 
YVPRPDKLKI RYYTVDGEEK TIRLKDYPAI VASHEIDHLN GHLFYDRINK QNPFELAEDT 


IVIS 

« Hide

References

[1]"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533."
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CNCM I-1225 / La1 / NCC 533.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017198 Genomic DNA. Translation: AAS08815.1.
RefSeqNP_964849.1. NC_005362.1.

3D structure databases

ProteinModelPortalQ74JW2.
SMRQ74JW2. Positions 1-183.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257314.LJ0993.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS08815; AAS08815; LJ_0993.
GeneID2743557.
KEGGljo:LJ0993.
PATRIC22238350. VBILacJoh1832_0853.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0242.
KOK01462.
OMASQDPKIA.
OrthoDBEOG6PZXGQ.

Enzyme and pathway databases

BioCycLJOH257314:GJN3-858-MONOMER.

Family and domain databases

Gene3D3.90.45.10. 1 hit.
HAMAPMF_00163. Pep_deformylase.
InterProIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERPTHR10458. PTHR10458. 1 hit.
PfamPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFPIRSF004749. Pep_def. 1 hit.
PRINTSPR01576. PDEFORMYLASE.
SUPFAMSSF56420. SSF56420. 1 hit.
TIGRFAMsTIGR00079. pept_deformyl. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDEF_LACJO
AccessionPrimary (citable) accession number: Q74JW2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families