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Q74JC1 (FTHS_LACJO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:LJ_1188
OrganismLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]
Taxonomic identifier257314 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length557 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 557557Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000199353

Regions

Nucleotide binding66 – 738ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q74JC1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 7E615C183F9F3393

FASTA55760,475
        10         20         30         40         50         60 
MKSDIEIAQD TKELPITEIA KKVDLQPDEI ELYGNDKAKI SWKGINRIKQ GKKLGKLILV 

        70         80         90        100        110        120 
TSISPTPAGE GKSTITIGLG DAISNQLHKN TLIALREPSM GPVFGLKGGA TGGGYAQIIT 

       130        140        150        160        170        180 
MEDINLHFTG DMHALTSAID TLAALVDNYI YQDNSLELDP NRILLKRGID VNDRTLRKIT 

       190        200        210        220        230        240 
IGQGSRFNGI EHEASFAITV ANELMAILCL ATDIDDLKKR IGNMLVGFSV KDEPVYVKDL 

       250        260        270        280        290        300 
GFEGAIAALL STALKPNLVQ TLEHTPAIVH GGPFANIAHG ANSVIATNTA LHLSDYVLTE 

       310        320        330        340        350        360 
AGFGADLGGQ KFMDFVSNHL DKRPDAVVVV ATVRALKYQA EETTDHLDEE NIPALEKGFE 

       370        380        390        400        410        420 
NLKRHMENMA HYGVPVIVLI NKFASDTEQE LSKLKELVKA DGFECEVVSY HDEGSKGGIK 

       430        440        450        460        470        480 
AAEKVVELTN KASDFTSVYE PTDSVEEKIS KIAHNIYHAK DIEYSDKAKD QLAEIKKMGK 

       490        500        510        520        530        540 
DNLPVIMAKT QYSFTDKKSI LGAPKDFTLH VKNLALKNGA GFIVVATGSI LDMPGLPKYP 

       550 
AALDIDVDNN GKISGLF 

« Hide

References

[1]"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533."
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CNCM I-1225 / La1 / NCC 533.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017198 Genomic DNA. Translation: AAS09009.1.
RefSeqNP_965043.1. NC_005362.1.

3D structure databases

ProteinModelPortalQ74JC1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257314.LJ1188.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS09009; AAS09009; LJ_1188.
GeneID2742818.
KEGGljo:LJ1188.
PATRIC22238753. VBILacJoh1832_1054.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
KOK01938.
OMADNYIYQD.
OrthoDBEOG6PCPSP.
ProtClustDBPRK13505.

Enzyme and pathway databases

BioCycLJOH257314:GJN3-1052-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_LACJO
AccessionPrimary (citable) accession number: Q74JC1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways