Reviewed, UniProtKB/Swiss-Prot Q74J32 (PYRC_LACJO)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	LJ_1278

Organism

Lactobacillus johnsonii [Complete proteome] [HAMAP]

Taxonomic identifier

33959 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Dihydroorotase		PRO_1000024090
Sites
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 1 By similarity
	Metal binding	138	1	Zinc 1; via carbamate group By similarity
	Metal binding	138	1	Zinc 2; via carbamate group By similarity
	Metal binding	175	1	Zinc 2 By similarity
	Metal binding	228	1	Zinc 2 By similarity
	Metal binding	301	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	138	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q74J32-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 99C2F52414311027	FASTA	425	46,053
	10 20 30 40 50 60 MTTVIKNGTV YQNGHLIKAD VLIDGQKIKA IGTDLEGEEI IDASGMIVSP GLVDVHVHYR 70 80 90 100 110 120 DPGQTYKEDI KTGSQAAARG GFTTVGAMPN VTPVPNTAEL MDKMVRENQK KGLVHILQYG 130 140 150 160 170 180 PVTNDETTDI IPDYAALKKA GAFALSNDGH GVQSAQTMYL AMQKAKENNL IIAAHAQDDS 190 200 210 220 230 240 LFNKGIVNEG IAAEKLDLPP VTELAETTQI ARDLLLAQKT GVHYHICHVS TKTSVELVRL 250 260 270 280 290 300 AKARGIKVTC EVAPHHILLT DSDIPEDNAY FKMNPPLRSK EDQVALLVGL LDGTIDLIAT 310 320 330 340 350 360 DHAPHAKAEK QGGMRGAAFG ITGSETAFST LYTRFVKEEK VFTLEQLLSL LTDKPATVFG 370 380 390 400 410 420 IENAGLLAPG ENADIAIFDI EHQREIKEDD FKSKGVNTPF TGHKVYGETV MTFVDGKVVY QRGTK

References

[1]

"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533." Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A. Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed: 14983040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 533.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017198 Genomic DNA. Translation: AAS09099.1.
RefSeq	NP_965133.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2743536.
GenomeReviews	Gene locus LJ_1278 in contig AE017198_GR.
KEGG	ljo:LJ1278.
NMPDR	fig|257314.1.peg.1119.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q74J32.
Enzyme and pathway databases
BioCyc	LJOH257314:LJ_1278-MON.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. False negative. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_LACJO

Accession

Primary (citable) accession number: Q74J32

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	September 2, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)