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Q74IU3

- TYSY_LACJO

UniProt

Q74IU3 - TYSY_LACJO

Protein

Thymidylate synthase

Gene

thyA

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei200 – 2001UniRule annotation

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. dTMP biosynthetic process Source: UniProtKB-HAMAP
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciLJOH257314:GJN3-1284-MONOMER.
    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
    Short name:
    TSUniRule annotation
    Short name:
    TSaseUniRule annotation
    Gene namesi
    Name:thyAUniRule annotation
    Ordered Locus Names:LJ_1471
    OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
    Taxonomic identifieri257314 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000000581: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 318318Thymidylate synthasePRO_0000140968Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi257314.LJ1471.

    Structurei

    3D structure databases

    ProteinModelPortaliQ74IU3.
    SMRiQ74IU3. Positions 4-318.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0207.
    KOiK00560.
    OMAiDVHLYSN.
    OrthoDBiEOG6K6V53.

    Family and domain databases

    Gene3Di3.30.572.10. 2 hits.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q74IU3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATLEQPYLD LLSKIMSEGH DKEDRTGTGT RSLFGAQMRF DLNDGFPILT    50
    TKKIPFGLIK SELLWFLRGD TNIRFLLEHN NHIWDEWAFK NWVESNEYQG 100
    PDMTNFGLRS QQDPEFKRIY QEEMKKFDQK VLEDQTFAEK YGNLGDVYGA 150
    QWRHWQKREG GFIDQIQNVI DQIKKTPYSR RLIVSAWNPE DVPTSALPPC 200
    HVLFQFYVND GCLSLQLYQR SGDMFLGVPF NIASYALLVN LVARETGLKP 250
    GEFIHTLGDA HIYKNHFNQV KELLNRSAYD APMLWLNPDK KLVQDFEMKD 300
    IKLINYRHHG TIKAPVAV 318
    Length:318
    Mass (Da):37,037
    Last modified:July 5, 2004 - v1
    Checksum:i6F634B76D9D1569D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017198 Genomic DNA. Translation: AAS09239.1.
    RefSeqiNP_965273.1. NC_005362.1.

    Genome annotation databases

    EnsemblBacteriaiAAS09239; AAS09239; LJ_1471.
    GeneIDi2743407.
    KEGGiljo:LJ1471.
    PATRICi22239225. VBILacJoh1832_1288.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017198 Genomic DNA. Translation: AAS09239.1 .
    RefSeqi NP_965273.1. NC_005362.1.

    3D structure databases

    ProteinModelPortali Q74IU3.
    SMRi Q74IU3. Positions 4-318.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257314.LJ1471.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAS09239 ; AAS09239 ; LJ_1471 .
    GeneIDi 2743407.
    KEGGi ljo:LJ1471.
    PATRICi 22239225. VBILacJoh1832_1288.

    Phylogenomic databases

    eggNOGi COG0207.
    KOi K00560.
    OMAi DVHLYSN.
    OrthoDBi EOG6K6V53.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .
    BioCyci LJOH257314:GJN3-1284-MONOMER.

    Family and domain databases

    Gene3Di 3.30.572.10. 2 hits.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CNCM I-1225 / La1 / NCC 533.

    Entry informationi

    Entry nameiTYSY_LACJO
    AccessioniPrimary (citable) accession number: Q74IU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3