ID   Q74IN2_LACJO            Unreviewed;       662 AA.
AC   Q74IN2;
DT   05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2004, sequence version 1.
DT   27-MAR-2024, entry version 135.
DE   SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:AAS09305.1};
GN   OrderedLocusNames=LJ_1537 {ECO:0000313|EMBL:AAS09305.1};
OS   Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533).
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=257314 {ECO:0000313|EMBL:AAS09305.1, ECO:0000313|Proteomes:UP000000581};
RN   [1] {ECO:0000313|EMBL:AAS09305.1, ECO:0000313|Proteomes:UP000000581}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CNCM I-1225 / La1 / NCC 533
RC   {ECO:0000313|Proteomes:UP000000581};
RX   PubMed=14983040; DOI=10.1073/pnas.0307327101;
RA   Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C.,
RA   Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R.,
RA   Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.;
RT   "The genome sequence of the probiotic intestinal bacterium Lactobacillus
RT   johnsonii NCC 533.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00001433};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1; Evidence={ECO:0000256|ARBA:ARBA00000775};
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DR   EMBL; AE017198; AAS09305.1; -; Genomic_DNA.
DR   RefSeq; WP_011162261.1; NC_005362.1.
DR   AlphaFoldDB; Q74IN2; -.
DR   KEGG; ljo:LJ_1537; -.
DR   PATRIC; fig|257314.6.peg.1355; -.
DR   eggNOG; COG0515; Bacteria.
DR   HOGENOM; CLU_000288_135_2_9; -.
DR   Proteomes; UP000000581; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-EC.
DR   CDD; cd06577; PASTA_pknB; 3.
DR   CDD; cd14014; STKc_PknB_like; 1.
DR   Gene3D; 2.60.40.2560; -; 1.
DR   Gene3D; 3.30.10.20; -; 3.
DR   Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR005543; PASTA_dom.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   NCBIfam; NF033483; PknB_PASTA_kin; 1.
DR   PANTHER; PTHR24345; SERINE/THREONINE-PROTEIN KINASE PLK; 1.
DR   PANTHER; PTHR24345:SF0; SERINE_THREONINE-PROTEIN KINASE PLK1; 1.
DR   Pfam; PF03793; PASTA; 3.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00740; PASTA; 3.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR   PROSITE; PS51178; PASTA; 3.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW   ProRule:PRU10141}; Membrane {ECO:0000256|SAM:Phobius};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW   ProRule:PRU10141}; Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        331..351
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          11..281
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000259|PROSITE:PS50011"
FT   DOMAIN          353..420
FT                   /note="PASTA"
FT                   /evidence="ECO:0000259|PROSITE:PS51178"
FT   DOMAIN          421..487
FT                   /note="PASTA"
FT                   /evidence="ECO:0000259|PROSITE:PS51178"
FT   DOMAIN          498..564
FT                   /note="PASTA"
FT                   /evidence="ECO:0000259|PROSITE:PS51178"
FT   REGION          453..476
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          526..556
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         40
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ   SEQUENCE   662 AA;  73529 MW;  6E3922AFD2440FF3 CRC64;
     MEKGHLLGGR YKIISVLGEG GMANVYLAED IILQRKVAVK VLRLDLQKDP QTIQRFQREA
     LSTSELSHPH IVSILDVGTD GNCHYLVMDY VDGSDLEEYI QHNNPIPLPK VIDIMDQILS
     AVALAHKHNV IHRDLKPQNI LMDKKGNIKI VDFGIAIALN QSTMTQTNTA MGSVHYMSPE
     QARGSMATKQ SDIYSLGIIL YKLLTGTVPF TGENAVAVAL KHFQEKTPSL RDKNPNVPQA
     LENVVFKATA KDPRDRYKSA LEMKADLDTC LDPKRKDEPI FKPEHDPSTD ETIVVPPLEG
     SVHNKDHEVQ AKEEPKEEKK SLFKNLKGHT WWWLGAIIAF CVIMVILFFA LGRKDMVDIP
     NINRMSQTQA KKSLQSSNLK IGKITYEYSD SVPKGEVIRT DPPIGSQIKD GEKVNLVLSR
     GSHLVEMPNV IGESYSLARK QLVKLGFTVT KTEEYSPSPK NQVNAQDIEP GQEVNPNKST
     VTLLVSKGPS SASSTRSRRN TIKLRDIVGY SLKGARDYAR EHHLSLKVEE EDSDGKNDGT
     VLRQSPESGT DVNPGDRLTV VVAKAKRNDS TDTSSSSTTT TTVTKSYTIN YPGSADQSGS
     EKTPDHIQVY VSDDGHSINN IYKDMNITSE QTFTIPFKLS KKNGHIKILR NGETILDEDV
     NK
//