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Q74I98

- TRMB_LACJO

UniProt

Q74I98 - TRMB_LACJO

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, LJ_1667
Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461S-adenosyl-L-methionine By similarity
Binding sitei71 – 711S-adenosyl-L-methionine By similarity
Binding sitei98 – 981S-adenosyl-L-methionine By similarity
Active sitei120 – 1201 By similarity
Binding sitei120 – 1201S-adenosyl-L-methionine By similarity
Binding sitei124 – 1241Substrate By similarity
Binding sitei156 – 1561Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLJOH257314:GJN3-1486-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:LJ_1667
OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Taxonomic identifieri257314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000581: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
PRO_0000171337Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi257314.LJ1667.

Structurei

3D structure databases

ProteinModelPortaliQ74I98.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1316Interaction with RNA Reviewed prediction
Regioni196 – 1994Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q74I98-1 [UniParc]FASTAAdd to Basket

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MRLRNKPWAQ KLVAEHPEVI LNEPDPDKKI NWEERFEDFS KPLAIEIGSG    50
KGQFITTLAK EHPEMNFIGV ELQTTAAGMI LRTKLEEKID NLQLMCADAA 100
NIAMYLPENS VDIVYLNFSD PWPKTRHEKR RLTYKSFLDK YRQILKPEGH 150
LEFKTDNRGL FEYSLVSLNN YGMKFDYVSL DLHHADDEIF ERNVETEYEH 200
KFAAKGNPIY CLHAHFVK 218
Length:218
Mass (Da):25,533
Last modified:July 5, 2004 - v1
Checksum:i717A915A1C869AD2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017198 Genomic DNA. Translation: AAS09440.1.
RefSeqiNP_965474.1. NC_005362.1.

Genome annotation databases

EnsemblBacteriaiAAS09440; AAS09440; LJ_1667.
GeneIDi2742184.
KEGGiljo:LJ1667.
PATRICi22239637. VBILacJoh1832_1493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017198 Genomic DNA. Translation: AAS09440.1 .
RefSeqi NP_965474.1. NC_005362.1.

3D structure databases

ProteinModelPortali Q74I98.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 257314.LJ1667.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS09440 ; AAS09440 ; LJ_1667 .
GeneIDi 2742184.
KEGGi ljo:LJ1667.
PATRICi 22239637. VBILacJoh1832_1493.

Phylogenomic databases

eggNOGi COG0220.
KOi K03439.
OMAi DNVMTEY.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci LJOH257314:GJN3-1486-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNCM I-1225 / La1 / NCC 533.

Entry informationi

Entry nameiTRMB_LACJO
AccessioniPrimary (citable) accession number: Q74I98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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