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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461S-adenosyl-L-methionineUniRule annotation
Binding sitei71 – 711S-adenosyl-L-methionineUniRule annotation
Binding sitei98 – 981S-adenosyl-L-methionineUniRule annotation
Active sitei120 – 1201By similarity
Binding sitei120 – 1201S-adenosyl-L-methionineUniRule annotation
Binding sitei124 – 1241SubstrateUniRule annotation
Binding sitei156 – 1561SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLJOH257314:GJN3-1486-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:LJ_1667
OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Taxonomic identifieri257314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000581 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218tRNA (guanine-N(7)-)-methyltransferasePRO_0000171337Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi257314.LJ1667.

Structurei

3D structure databases

ProteinModelPortaliQ74I98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1316Interaction with RNAUniRule annotation
Regioni196 – 1994Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiAHPEINY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q74I98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRNKPWAQ KLVAEHPEVI LNEPDPDKKI NWEERFEDFS KPLAIEIGSG
60 70 80 90 100
KGQFITTLAK EHPEMNFIGV ELQTTAAGMI LRTKLEEKID NLQLMCADAA
110 120 130 140 150
NIAMYLPENS VDIVYLNFSD PWPKTRHEKR RLTYKSFLDK YRQILKPEGH
160 170 180 190 200
LEFKTDNRGL FEYSLVSLNN YGMKFDYVSL DLHHADDEIF ERNVETEYEH
210
KFAAKGNPIY CLHAHFVK
Length:218
Mass (Da):25,533
Last modified:July 5, 2004 - v1
Checksum:i717A915A1C869AD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017198 Genomic DNA. Translation: AAS09440.1.
RefSeqiWP_011162354.1. NC_005362.1.

Genome annotation databases

EnsemblBacteriaiAAS09440; AAS09440; LJ_1667.
GeneIDi2742184.
KEGGiljo:LJ1667.
PATRICi22239637. VBILacJoh1832_1493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017198 Genomic DNA. Translation: AAS09440.1.
RefSeqiWP_011162354.1. NC_005362.1.

3D structure databases

ProteinModelPortaliQ74I98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257314.LJ1667.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS09440; AAS09440; LJ_1667.
GeneIDi2742184.
KEGGiljo:LJ1667.
PATRICi22239637. VBILacJoh1832_1493.

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiAHPEINY.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciLJOH257314:GJN3-1486-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNCM I-1225 / La1 / NCC 533.

Entry informationi

Entry nameiTRMB_LACJO
AccessioniPrimary (citable) accession number: Q74I98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.