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Q74HC3

- DGK1_LACJO

UniProt

Q74HC3 - DGK1_LACJO

Protein

Deoxyadenosine kinase

Gene

LJ_1824

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    DGK/DAK plays an essential role in generating the deoxyribonucleotide precursors, dGTP and dATP, for DNA metabolism.

    Catalytic activityi

    ATP + deoxyadenosine = ADP + dAMP.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321SubstrateBy similarity
    Binding sitei44 – 441SubstrateBy similarity
    Binding sitei55 – 551SubstrateBy similarity
    Active sitei78 – 781Proton acceptorSequence Analysis
    Binding sitei79 – 791SubstrateBy similarity
    Binding sitei84 – 841SubstrateBy similarity
    Binding sitei149 – 1491SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 169ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. deoxyadenosine kinase activity Source: UniProtKB-EC
    3. phosphotransferase activity, alcohol group as acceptor Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciLJOH257314:GJN3-1886-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyadenosine kinase (EC:2.7.1.76)
    Short name:
    DADO kinase
    Short name:
    DAK
    Alternative name(s):
    Deoxynucleoside kinase complex I S-component
    Gene namesi
    Ordered Locus Names:LJ_1824
    OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
    Taxonomic identifieri257314 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000000581: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 215214Deoxyadenosine kinasePRO_0000239236Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of a deoxyadenosine (DAK) and a deoxyguanosine kinase (DGK).

    Protein-protein interaction databases

    STRINGi257314.LJ1824.

    Structurei

    3D structure databases

    ProteinModelPortaliQ74HC3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DCK/DGK family.Curated

    Phylogenomic databases

    eggNOGiCOG1428.
    OMAiLLIYIHV.
    OrthoDBiEOG628F9B.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10513. PTHR10513. 1 hit.
    PfamiPF01712. dNK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000705. DNK. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q74HC3-1 [UniParc]FASTAAdd to Basket

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    MTVIVLSGPI GAGKSSLTSL LAEHLGTQAF YEGVDNNPIL PLYYKDMAHY    50
    TFLLNTYLLN HRLAQINQAI RDHNSVSDRS IYEDALFFKM NVDSGIADPT 100
    EFKIYDSLLE NMMEQAPGNP SKKPDLLIYI HVSLDTMLHR IQKRGRKFEQ 150
    LSTDPSLKDY YARLLSYYEP WYEKYNASPK MMIDGDKYDF VANEDARRKV 200
    INAIDQKLID IGNLN 215
    Length:215
    Mass (Da):24,696
    Last modified:January 23, 2007 - v3
    Checksum:i5D19BF68370C1F96
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01881 Genomic DNA. Translation: AAB09750.1.
    AE017198 Genomic DNA. Translation: AAS09769.1.
    PIRiA56168.
    RefSeqiNP_965803.1. NC_005362.1.

    Genome annotation databases

    EnsemblBacteriaiAAS09769; AAS09769; LJ_1824.
    GeneIDi2742213.
    KEGGiljo:LJ1824.
    PATRICi22240444. VBILacJoh1832_1825.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01881 Genomic DNA. Translation: AAB09750.1 .
    AE017198 Genomic DNA. Translation: AAS09769.1 .
    PIRi A56168.
    RefSeqi NP_965803.1. NC_005362.1.

    3D structure databases

    ProteinModelPortali Q74HC3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257314.LJ1824.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAS09769 ; AAS09769 ; LJ_1824 .
    GeneIDi 2742213.
    KEGGi ljo:LJ1824.
    PATRICi 22240444. VBILacJoh1832_1825.

    Phylogenomic databases

    eggNOGi COG1428.
    OMAi LLIYIHV.
    OrthoDBi EOG628F9B.

    Enzyme and pathway databases

    BioCyci LJOH257314:GJN3-1886-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10513. PTHR10513. 1 hit.
    Pfami PF01712. dNK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000705. DNK. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the heterodimeric deoxyguanosine kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26."
      Ma G.T., Hong Y.S., Ives D.H.
      J. Biol. Chem. 270:6595-6601(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CNCM I-1225 / La1 / NCC 533.
    3. "Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26: functional assignment of subunits using limited proteolysis controlled by end-product inhibitors."
      Ikeda S., Ma G.T., Ives D.H.
      Biochemistry 33:5328-5334(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-38.
      Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.

    Entry informationi

    Entry nameiDGK1_LACJO
    AccessioniPrimary (citable) accession number: Q74HC3
    Secondary accession number(s): Q59483, Q5FHT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 66 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3