Q74HC3 (DGK1_LACJO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyadenosine kinase
Short name=DADO kinase
Short name=DAK
EC=2.7.1.76

Alternative name(s):
Deoxynucleoside kinase complex I S-component

Gene names

Ordered Locus Names:

LJ_1824

Organism

Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]

Taxonomic identifier

257314 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus johnsonii

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DGK/DAK plays an essential role in generating the deoxyribonucleotide precursors, dGTP and dATP, for DNA metabolism.
Catalytic activity	ATP + deoxyadenosine = ADP + dAMP.
Subunit structure	Heterodimer of a deoxyadenosine (DAK) and a deoxyguanosine kinase (DGK).
Sequence similarities	Belongs to the DCK/DGK family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	dAMP biosynthetic process Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW deoxyadenosine kinase activity Inferred from electronic annotation. Source: EC phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 215	214	Deoxyadenosine kinase		PRO_0000239236
Regions
	Nucleotide binding	8 – 16	9	ATP By similarity
Sites
	Active site	78	1	Proton acceptor Potential
	Binding site	32	1	Substrate By similarity
	Binding site	44	1	Substrate By similarity
	Binding site	55	1	Substrate By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	84	1	Substrate By similarity
	Binding site	149	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q74HC3 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5D19BF68370C1F96	FASTA	215	24,696
	10 20 30 40 50 60 MTVIVLSGPI GAGKSSLTSL LAEHLGTQAF YEGVDNNPIL PLYYKDMAHY TFLLNTYLLN 70 80 90 100 110 120 HRLAQINQAI RDHNSVSDRS IYEDALFFKM NVDSGIADPT EFKIYDSLLE NMMEQAPGNP 130 140 150 160 170 180 SKKPDLLIYI HVSLDTMLHR IQKRGRKFEQ LSTDPSLKDY YARLLSYYEP WYEKYNASPK 190 200 210 MMIDGDKYDF VANEDARRKV INAIDQKLID IGNLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of the heterodimeric deoxyguanosine kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26." Ma G.T., Hong Y.S., Ives D.H. J. Biol. Chem. 270:6595-6601(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.
[2]	"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533." Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A. Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CNCM I-1225 / La1 / NCC 533.
[3]	"Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26: functional assignment of subunits using limited proteolysis controlled by end-product inhibitors." Ikeda S., Ma G.T., Ives D.H. Biochemistry 33:5328-5334(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-38. Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01881 Genomic DNA. Translation: AAB09750.1. AE017198 Genomic DNA. Translation: AAS09769.1.
PIR	A56168.
RefSeq	NP_965803.1. NC_005362.1.
3D structure databases
ProteinModelPortal	Q74HC3.
ModBase	Search...
Protein-protein interaction databases
STRING	257314.LJ1824.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS09769; AAS09769; LJ_1824.
GeneID	2742213.
KEGG	ljo:LJ1824.
PATRIC	22240444. VBILacJoh1832_1825.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1428.
OMA	TYLLNHR.
ProtClustDB	CLSK925552.
Enzyme and pathway databases
BioCyc	LJOH257314:GJN3-1886-MONOMER.
Family and domain databases
InterPro	IPR002624. Deoxynucleoside_kinase. [Graphical view]
PANTHER	PTHR10513. PTHR10513. 1 hit.
Pfam	PF01712. dNK. 1 hit. [Graphical view]
PIRSF	PIRSF000705. DNK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DGK1_LACJO

Accession

Primary (citable) accession number: Q74HC3
Secondary accession number(s): Q59483, Q5FHT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 59 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program