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Protein

Deoxyadenosine kinase

Gene

LJ_1824

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DGK/DAK plays an essential role in generating the deoxyribonucleotide precursors, dGTP and dATP, for DNA metabolism.

Catalytic activityi

ATP + deoxyadenosine = ADP + dAMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321SubstrateBy similarity
Binding sitei44 – 441SubstrateBy similarity
Binding sitei55 – 551SubstrateBy similarity
Active sitei78 – 781Proton acceptorSequence Analysis
Binding sitei79 – 791SubstrateBy similarity
Binding sitei84 – 841SubstrateBy similarity
Binding sitei149 – 1491SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 169ATPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLJOH257314:GJN3-1886-MONOMER.
SABIO-RKQ74HC3.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyadenosine kinase (EC:2.7.1.76)
Short name:
DADO kinase
Short name:
DAK
Alternative name(s):
Deoxynucleoside kinase complex I S-component
Gene namesi
Ordered Locus Names:LJ_1824
OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Taxonomic identifieri257314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000581 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 215214Deoxyadenosine kinasePRO_0000239236Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a deoxyadenosine (DAK) and a deoxyguanosine kinase (DGK).

Protein-protein interaction databases

STRINGi257314.LJ1824.

Structurei

3D structure databases

ProteinModelPortaliQ74HC3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DCK/DGK family.Curated

Phylogenomic databases

eggNOGiCOG1428.
KOiK10353.
OMAiYKDMKRY.
OrthoDBiEOG628F9B.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10513. PTHR10513. 1 hit.
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q74HC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVIVLSGPI GAGKSSLTSL LAEHLGTQAF YEGVDNNPIL PLYYKDMAHY
60 70 80 90 100
TFLLNTYLLN HRLAQINQAI RDHNSVSDRS IYEDALFFKM NVDSGIADPT
110 120 130 140 150
EFKIYDSLLE NMMEQAPGNP SKKPDLLIYI HVSLDTMLHR IQKRGRKFEQ
160 170 180 190 200
LSTDPSLKDY YARLLSYYEP WYEKYNASPK MMIDGDKYDF VANEDARRKV
210
INAIDQKLID IGNLN
Length:215
Mass (Da):24,696
Last modified:January 23, 2007 - v3
Checksum:i5D19BF68370C1F96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01881 Genomic DNA. Translation: AAB09750.1.
AE017198 Genomic DNA. Translation: AAS09769.1.
PIRiA56168.
RefSeqiWP_041818540.1. NC_005362.1.

Genome annotation databases

EnsemblBacteriaiAAS09769; AAS09769; LJ_1824.
GeneIDi2742213.
KEGGiljo:LJ1824.
PATRICi22240444. VBILacJoh1832_1825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01881 Genomic DNA. Translation: AAB09750.1.
AE017198 Genomic DNA. Translation: AAS09769.1.
PIRiA56168.
RefSeqiWP_041818540.1. NC_005362.1.

3D structure databases

ProteinModelPortaliQ74HC3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257314.LJ1824.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS09769; AAS09769; LJ_1824.
GeneIDi2742213.
KEGGiljo:LJ1824.
PATRICi22240444. VBILacJoh1832_1825.

Phylogenomic databases

eggNOGiCOG1428.
KOiK10353.
OMAiYKDMKRY.
OrthoDBiEOG628F9B.

Enzyme and pathway databases

BioCyciLJOH257314:GJN3-1886-MONOMER.
SABIO-RKQ74HC3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10513. PTHR10513. 1 hit.
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the heterodimeric deoxyguanosine kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26."
    Ma G.T., Hong Y.S., Ives D.H.
    J. Biol. Chem. 270:6595-6601(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNCM I-1225 / La1 / NCC 533.
  3. "Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26: functional assignment of subunits using limited proteolysis controlled by end-product inhibitors."
    Ikeda S., Ma G.T., Ives D.H.
    Biochemistry 33:5328-5334(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-38.
    Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.

Entry informationi

Entry nameiDGK1_LACJO
AccessioniPrimary (citable) accession number: Q74HC3
Secondary accession number(s): Q59483, Q5FHT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 71 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.