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Q74HC2 (DGK2_LACJO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyguanosine kinase

Short name=DGK
Short name=DGUO kinase
EC=2.7.1.113
Alternative name(s):
Deoxynucleoside kinase complex I F-component
Gene names
Ordered Locus Names:LJ_1825
OrganismLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) [Complete proteome] [HAMAP]
Taxonomic identifier257314 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DGK/DAK plays an essential role in generating the deoxyribonucleotide precursors, dGTP and dATP, for DNA metabolism.

Catalytic activity

ATP + deoxyguanosine = ADP + dGMP.

Subunit structure

Heterodimer of a deoxyadenosine (DAK) and a deoxyguanosine kinase (DGK).

Sequence similarities

Belongs to the DCK/DGK family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 224223Deoxyguanosine kinase
PRO_0000239237

Regions

Nucleotide binding8 – 169ATP By similarity

Sites

Active site781Proton acceptor Potential
Binding site321Substrate By similarity
Binding site441Substrate By similarity
Binding site551Substrate By similarity
Binding site791Substrate By similarity
Binding site841Substrate By similarity
Binding site1491Substrate By similarity

Experimental info

Sequence conflict1731E → A in AAB09751. Ref.1
Sequence conflict2141F → L in AAB09751. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q74HC2 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8DD02003CF396CDD

FASTA22426,406
        10         20         30         40         50         60 
MTVIVLSGPI GAGKSSLTGI LSKYLGTNPF YESVDDNPVL PLFYENPKKY AFLLQVYFLN 

        70         80         90        100        110        120 
TRFRSIKSAL TDDNNVLDRS IYEDALFFQM NADIGRATPE EVDTYYELLH NMMSELDRMP 

       130        140        150        160        170        180 
KKNPDLLVHI DVSYDTMLKR IQKRGRNYEQ LSYDPTLEDY YKRLLRYYKP WYEKYDYSPK 

       190        200        210        220 
MTIDGDKLDF MASEEDRQEV LNQIVAKLKE MGKFEDDWKP NLVK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of the heterodimeric deoxyguanosine kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26."
Ma G.T., Hong Y.S., Ives D.H.
J. Biol. Chem. 270:6595-6601(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.
[2]"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533."
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CNCM I-1225 / La1 / NCC 533.
[3]"Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26: functional assignment of subunits using limited proteolysis controlled by end-product inhibitors."
Ikeda S., Ma G.T., Ives D.H.
Biochemistry 33:5328-5334(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-18.
Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U01881 Genomic DNA. Translation: AAB09751.1.
AE017198 Genomic DNA. Translation: AAS09770.1.
PIRB56168.
RefSeqNP_965804.1. NC_005362.1.

3D structure databases

ProteinModelPortalQ74HC2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257314.LJ1825.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS09770; AAS09770; LJ_1825.
GeneID2742011.
KEGGljo:LJ1825.
PATRIC22240446. VBILacJoh1832_1826.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1428.
OMAFERPQDN.
OrthoDBEOG628F9B.

Enzyme and pathway databases

BioCycLJOH257314:GJN3-1887-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR002624. Deoxynucleoside_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10513. PTHR10513. 1 hit.
PfamPF01712. dNK. 1 hit.
[Graphical view]
PIRSFPIRSF000705. DNK. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDGK2_LACJO
AccessionPrimary (citable) accession number: Q74HC2
Secondary accession number(s): Q59484, Q5FHS9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 65 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families