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Q74HC2

- DGK2_LACJO

UniProt

Q74HC2 - DGK2_LACJO

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Protein

Deoxyguanosine kinase

Gene

LJ_1825

Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

DGK/DAK plays an essential role in generating the deoxyribonucleotide precursors, dGTP and dATP, for DNA metabolism.

Catalytic activityi

ATP + deoxyguanosine = ADP + dGMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321SubstrateBy similarity
Binding sitei44 – 441SubstrateBy similarity
Binding sitei55 – 551SubstrateBy similarity
Active sitei78 – 781Proton acceptorSequence Analysis
Binding sitei79 – 791SubstrateBy similarity
Binding sitei84 – 841SubstrateBy similarity
Binding sitei149 – 1491SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 169ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. deoxyguanosine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLJOH257314:GJN3-1887-MONOMER.
SABIO-RKQ74HC2.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyguanosine kinase (EC:2.7.1.113)
Short name:
DGK
Short name:
DGUO kinase
Alternative name(s):
Deoxynucleoside kinase complex I F-component
Gene namesi
Ordered Locus Names:LJ_1825
OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Taxonomic identifieri257314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000581: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 224223Deoxyguanosine kinasePRO_0000239237Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a deoxyadenosine (DAK) and a deoxyguanosine kinase (DGK).

Protein-protein interaction databases

STRINGi257314.LJ1825.

Structurei

3D structure databases

ProteinModelPortaliQ74HC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DCK/DGK family.Curated

Phylogenomic databases

eggNOGiCOG1428.
OMAiFERPQDN.
OrthoDBiEOG628F9B.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10513. PTHR10513. 1 hit.
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q74HC2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVIVLSGPI GAGKSSLTGI LSKYLGTNPF YESVDDNPVL PLFYENPKKY
60 70 80 90 100
AFLLQVYFLN TRFRSIKSAL TDDNNVLDRS IYEDALFFQM NADIGRATPE
110 120 130 140 150
EVDTYYELLH NMMSELDRMP KKNPDLLVHI DVSYDTMLKR IQKRGRNYEQ
160 170 180 190 200
LSYDPTLEDY YKRLLRYYKP WYEKYDYSPK MTIDGDKLDF MASEEDRQEV
210 220
LNQIVAKLKE MGKFEDDWKP NLVK
Length:224
Mass (Da):26,406
Last modified:January 23, 2007 - v3
Checksum:i8DD02003CF396CDD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731E → A in AAB09751. (PubMed:7896798)Curated
Sequence conflicti214 – 2141F → L in AAB09751. (PubMed:7896798)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01881 Genomic DNA. Translation: AAB09751.1.
AE017198 Genomic DNA. Translation: AAS09770.1.
PIRiB56168.
RefSeqiNP_965804.1. NC_005362.1.

Genome annotation databases

EnsemblBacteriaiAAS09770; AAS09770; LJ_1825.
GeneIDi2742011.
KEGGiljo:LJ1825.
PATRICi22240446. VBILacJoh1832_1826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01881 Genomic DNA. Translation: AAB09751.1 .
AE017198 Genomic DNA. Translation: AAS09770.1 .
PIRi B56168.
RefSeqi NP_965804.1. NC_005362.1.

3D structure databases

ProteinModelPortali Q74HC2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 257314.LJ1825.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS09770 ; AAS09770 ; LJ_1825 .
GeneIDi 2742011.
KEGGi ljo:LJ1825.
PATRICi 22240446. VBILacJoh1832_1826.

Phylogenomic databases

eggNOGi COG1428.
OMAi FERPQDN.
OrthoDBi EOG628F9B.

Enzyme and pathway databases

BioCyci LJOH257314:GJN3-1887-MONOMER.
SABIO-RK Q74HC2.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10513. PTHR10513. 1 hit.
Pfami PF01712. dNK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000705. DNK. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the heterodimeric deoxyguanosine kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26."
    Ma G.T., Hong Y.S., Ives D.H.
    J. Biol. Chem. 270:6595-6601(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNCM I-1225 / La1 / NCC 533.
  3. "Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26: functional assignment of subunits using limited proteolysis controlled by end-product inhibitors."
    Ikeda S., Ma G.T., Ives D.H.
    Biochemistry 33:5328-5334(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-18.
    Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.

Entry informationi

Entry nameiDGK2_LACJO
AccessioniPrimary (citable) accession number: Q74HC2
Secondary accession number(s): Q59484, Q5FHS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 68 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3