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Q74HC2

- DGK2_LACJO

UniProt

Q74HC2 - DGK2_LACJO

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Protein

Deoxyguanosine kinase

Gene
LJ_1825
Organism
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

DGK/DAK plays an essential role in generating the deoxyribonucleotide precursors, dGTP and dATP, for DNA metabolism.

Catalytic activityi

ATP + deoxyguanosine = ADP + dGMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321Substrate By similarity
Binding sitei44 – 441Substrate By similarity
Binding sitei55 – 551Substrate By similarity
Active sitei78 – 781Proton acceptor Reviewed prediction
Binding sitei79 – 791Substrate By similarity
Binding sitei84 – 841Substrate By similarity
Binding sitei149 – 1491Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 169ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. deoxyguanosine kinase activity Source: UniProtKB-EC
  3. phosphotransferase activity, alcohol group as acceptor Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLJOH257314:GJN3-1887-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyguanosine kinase (EC:2.7.1.113)
Short name:
DGK
Short name:
DGUO kinase
Alternative name(s):
Deoxynucleoside kinase complex I F-component
Gene namesi
Ordered Locus Names:LJ_1825
OrganismiLactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Taxonomic identifieri257314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000581: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 224223Deoxyguanosine kinasePRO_0000239237Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a deoxyadenosine (DAK) and a deoxyguanosine kinase (DGK).

Protein-protein interaction databases

STRINGi257314.LJ1825.

Structurei

3D structure databases

ProteinModelPortaliQ74HC2.

Family & Domainsi

Sequence similaritiesi

Belongs to the DCK/DGK family.

Phylogenomic databases

eggNOGiCOG1428.
OMAiFERPQDN.
OrthoDBiEOG628F9B.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10513. PTHR10513. 1 hit.
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q74HC2-1 [UniParc]FASTAAdd to Basket

« Hide

MTVIVLSGPI GAGKSSLTGI LSKYLGTNPF YESVDDNPVL PLFYENPKKY    50
AFLLQVYFLN TRFRSIKSAL TDDNNVLDRS IYEDALFFQM NADIGRATPE 100
EVDTYYELLH NMMSELDRMP KKNPDLLVHI DVSYDTMLKR IQKRGRNYEQ 150
LSYDPTLEDY YKRLLRYYKP WYEKYDYSPK MTIDGDKLDF MASEEDRQEV 200
LNQIVAKLKE MGKFEDDWKP NLVK 224
Length:224
Mass (Da):26,406
Last modified:January 23, 2007 - v3
Checksum:i8DD02003CF396CDD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731E → A in AAB09751. 1 Publication
Sequence conflicti214 – 2141F → L in AAB09751. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01881 Genomic DNA. Translation: AAB09751.1.
AE017198 Genomic DNA. Translation: AAS09770.1.
PIRiB56168.
RefSeqiNP_965804.1. NC_005362.1.

Genome annotation databases

EnsemblBacteriaiAAS09770; AAS09770; LJ_1825.
GeneIDi2742011.
KEGGiljo:LJ1825.
PATRICi22240446. VBILacJoh1832_1826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01881 Genomic DNA. Translation: AAB09751.1 .
AE017198 Genomic DNA. Translation: AAS09770.1 .
PIRi B56168.
RefSeqi NP_965804.1. NC_005362.1.

3D structure databases

ProteinModelPortali Q74HC2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 257314.LJ1825.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS09770 ; AAS09770 ; LJ_1825 .
GeneIDi 2742011.
KEGGi ljo:LJ1825.
PATRICi 22240446. VBILacJoh1832_1826.

Phylogenomic databases

eggNOGi COG1428.
OMAi FERPQDN.
OrthoDBi EOG628F9B.

Enzyme and pathway databases

BioCyci LJOH257314:GJN3-1887-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10513. PTHR10513. 1 hit.
Pfami PF01712. dNK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000705. DNK. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the heterodimeric deoxyguanosine kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26."
    Ma G.T., Hong Y.S., Ives D.H.
    J. Biol. Chem. 270:6595-6601(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNCM I-1225 / La1 / NCC 533.
  3. "Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26: functional assignment of subunits using limited proteolysis controlled by end-product inhibitors."
    Ikeda S., Ma G.T., Ives D.H.
    Biochemistry 33:5328-5334(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-18.
    Strain: ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088.

Entry informationi

Entry nameiDGK2_LACJO
AccessioniPrimary (citable) accession number: Q74HC2
Secondary accession number(s): Q59484, Q5FHS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 66 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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