Q74GA8 (NUOA1_GEOSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit A 1
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit A 1
NDH-1 subunit A 1
NUO1 1

Gene names

Name:	nuoA1
Ordered Locus Names:	GSU0338

Organism

Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)

Taxonomic identifier

243231 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	NADH-quinone oxidoreductase subunit A 1		PRO_0000362693
Regions
	Transmembrane	1 – 21	21	Helical; Potential
	Transmembrane	60 – 80	21	Helical; Potential
	Transmembrane	87 – 107	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q74GA8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0C5B3A291A4E343C	FASTA	118	13,254
	10 20 30 40 50 60 MLGVYLPIIV LVAVAVIFGL ASLTFSSLIG QKKPSAVKLA PYECGCEPVG SARERFSVKF 70 80 90 100 110 YIIAMLFILF DIEAVFMYPW SVLFKRLGIF GVVEMGLFIV ILFVGYIYVW KKGALEWE

References

[1]

"Genome of Geobacter sulfurreducens: metal reduction in subsurface environments." Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C., Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J., Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn M.L., Kolonay J.F. , Sullivan S.A., Haft D.H., Selengut J., Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A., Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., Van Aken S.E., Lovley D.R., Fraser C.M. Science 302:1967-1969(2003) [PubMed: 14671304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51573 / DSM 12127 / PCA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017180 Genomic DNA. Translation: AAR33671.1.
RefSeq	NP_951398.1. NC_002939.4.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2686820.
GenomeReviews	Gene locus GSU0338 in contig AE017180_GR.
KEGG	gsu:GSU0338.
NMPDR	fig|243231.1.peg.336.
PATRIC	22023418. VBIGeoSul17553_0335.
TIGR	GSU0338.
Phylogenomic databases
HOGENOM	HBG605540.
OMA	IGFAYEW.
PhylomeDB	Q74GA8.
ProtClustDB	CLSK827761.
Enzyme and pathway databases
BioCyc	GSUL243231:GSU_0338-MONOMER.
Family and domain databases
InterPro	IPR023043. NAD(P)H_OxRDtase_bac/plastid. IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
KO	K00330.
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA1_GEOSL

Accession

Primary (citable) accession number: Q74GA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program