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Q74FT5 (TRMB_GEOSL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:GSU0521
OrganismGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) [Reference proteome] [HAMAP]
Taxonomic identifier243231 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter

Protein attributes

Sequence length209 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 209209tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171331

Sites

Active site1121 By similarity
Binding site371S-adenosyl-L-methionine By similarity
Binding site621S-adenosyl-L-methionine By similarity
Binding site891S-adenosyl-L-methionine By similarity
Binding site1121S-adenosyl-L-methionine By similarity
Binding site1161Substrate By similarity
Binding site1481Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q74FT5 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 553804A6D158D6BA

FASTA20924,130
        10         20         30         40         50         60 
MQRIIPIDSP FFLNPDELGT PADWTAVFGN DNPLALEIGC GIGDFIARTA LDHPTTNFIA 

        70         80         90        100        110        120 
IDYYNKGCLK TCSRLERHGI TNVRVVRAEA RQFIVERIQK GSLAAVYINC PDPWPKKRHR 

       130        140        150        160        170        180 
KRRLVNTQFV RFIRDYLAPG GDFYFATDFD DYGEDVAAFM PQMPGYANML APDIFRHELE 

       190        200 
GYHLSKYMQK FMAEGKRIYF IHYRTTMEG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017180 Genomic DNA. Translation: AAR33852.1.
RefSeqNP_951579.1. NC_002939.5.

3D structure databases

ProteinModelPortalQ74FT5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243231.GSU0521.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAR33852; AAR33852; GSU0521.
GeneID2685959.
KEGGgsu:GSU0521.
PATRIC22023797. VBIGeoSul17553_0522.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000134942.
KOK03439.
OMAAEWIASH.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK827898.

Enzyme and pathway databases

BioCycGSUL243231:GH27-526-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_GEOSL
AccessionPrimary (citable) accession number: Q74FT5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways