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Protein

Thiazole synthase

Gene

thiG

Organism
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102Schiff-base intermediate with DXPUniRule annotation1
Binding sitei163DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

  • catalytic activity Source: TIGR
  • transferase activity Source: UniProtKB-KW

GO - Biological processi

  • thiamine biosynthetic process Source: TIGR
  • thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:GSU0588
OrganismiGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Taxonomic identifieri243231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000000577 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001628231 – 260Thiazole synthaseAdd BLAST260

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi243231.GSU0588.

Structurei

3D structure databases

ProteinModelPortaliQ74FL9.
SMRiQ74FL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni189 – 190DXP bindingUniRule annotation2
Regioni211 – 212DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
HOGENOMiHOG000248049.
InParanoidiQ74FL9.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiPOG091H01K9.

Family and domain databases

CDDicd04728. ThiG. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR033983. Thiazole_synthase_ThiG.
IPR008867. ThiG.
PfamiView protein in Pfam
PF05690. ThiG. 1 hit.

Sequencei

Sequence statusi: Complete.

Q74FL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNAADKLVI AGREFSSRLM VGTGKYASNE QMIKALEVSG AEIITVAVRR
60 70 80 90 100
VNLADRGKGC LLDFIDPKKY TLLPNTAGCY TADDAVRTCR LAREAGMSDL
110 120 130 140 150
VKLEVLGDEK TLFPDNEELL KAAKILVKEG FTVLPYTSDD PIVCKKLEDI
160 170 180 190 200
GCAAVMPLGA PIGSGLGIRN PYNILIIKET VKVPVIVDAG VGTASDAAIA
210 220 230 240 250
MELGIDGVLM NTGIAGARDP IAMAEAMNMA VRAGRLAYLA GRIPKKLYAT
260
ASSPIEGMIG
Length:260
Mass (Da):27,432
Last modified:July 5, 2004 - v1
Checksum:i13188B58DDF4DA4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR33919.1.
RefSeqiNP_951646.1. NC_002939.5.
WP_010941251.1. NC_002939.5.

Genome annotation databases

EnsemblBacteriaiAAR33919; AAR33919; GSU0588.
GeneIDi2685792.
KEGGigsu:GSU0588.
PATRICifig|243231.5.peg.586.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTHIG_GEOSL
AccessioniPrimary (citable) accession number: Q74FL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 5, 2004
Last modified: June 7, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families