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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi484MagnesiumUniRule annotation1
Metal bindingi490MagnesiumUniRule annotation1

GO - Molecular functioni

  • 3'-5'-exoribonuclease activity Source: InterPro
  • magnesium ion binding Source: UniProtKB-HAMAP
  • mRNA binding Source: TIGR
  • polyribonucleotide nucleotidyltransferase activity Source: TIGR

GO - Biological processi

  • mRNA catabolic process Source: TIGR
  • RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:GSU1593
OrganismiGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Taxonomic identifieri243231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000000577 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003296621 – 697Polyribonucleotide nucleotidyltransferaseAdd BLAST697

Interactioni

Protein-protein interaction databases

STRINGi243231.GSU1593.

Structurei

3D structure databases

ProteinModelPortaliQ74CS9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 610KHUniRule annotationAdd BLAST60
Domaini620 – 688S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
InParanoidiQ74CS9.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q74CS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEHKVNVEF GGRTITIATG KWAKQASGAV VVSCGDTVVL VTAVATKSAR
60 70 80 90 100
EGQDFFPLTV NYQEKAYAGG KIPGGFFKRE GRPSDNETLT SRFIDRPIRP
110 120 130 140 150
LFPESFLNDT QIMATVVSAD QDNDPGILAM IGASAALEVS DIPFLGPIAG
160 170 180 190 200
VKVGRVDGQF VCNPTVEQLE KSDLEIVVAA SRDAVIMVEG GAAEASEKDV
210 220 230 240 250
LEAIFFGHAA VQPIIEAQTD LRKLAGVPKR EVAATSVDEA LKTRVKDLAY
260 270 280 290 300
AGIKEAVRIV AKQERHNRIG EITAQTLETL LPEYEGRESE IKGFLGDFEY
310 320 330 340 350
ELVREHIIKD GYRIDGRDTT TIRPISIEVS MLPRAHGSAL FTRGETQALV
360 370 380 390 400
ASTLGTSIDE QRIDSLYGET RKRFLLHYNF PPFSVGETSF RLAPGRREIG
410 420 430 440 450
HGMLAERALE RVVPKHEDFP YTIRIVSDIL ESNGSSSMAT VCGGALAMMD
460 470 480 490 500
AGVPIKAPVA GIAMGLIKEG EGIAILSDIL GDEDHLGDMD FKVAGTEAGV
510 520 530 540 550
TAIQMDIKIT GVTREIMEKA LLQARDGRLH ILGKMNQAIA APRTDLSPYA
560 570 580 590 600
PRITTIWVKT DKIRDVIGSG GKNIRGITEA TGVSIDIEDS GRINIASTSK
610 620 630 640 650
EACDKAIKMI RDLTAEAEEG KLYMGTVKKV MDFGAFVEIF PGTDGLVHIS
660 670 680 690
ELDTERVKNV TDVLNEGDKV LVKCIGIDKQ GKIKLSRKEA LGAVLPE
Length:697
Mass (Da):75,348
Last modified:July 5, 2004 - v1
Checksum:i23D722D1F0335F12
GO

Sequence cautioni

The sequence AAR34967 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR34967.2. Different initiation.
RefSeqiNP_952644.4. NC_002939.5.

Genome annotation databases

EnsemblBacteriaiAAR34967; AAR34967; GSU1593.
GeneIDi2687125.
KEGGigsu:GSU1593.
PATRICi22026031. VBIGeoSul17553_1634.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR34967.2. Different initiation.
RefSeqiNP_952644.4. NC_002939.5.

3D structure databases

ProteinModelPortaliQ74CS9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243231.GSU1593.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAR34967; AAR34967; GSU1593.
GeneIDi2687125.
KEGGigsu:GSU1593.
PATRICi22026031. VBIGeoSul17553_1634.

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
InParanoidiQ74CS9.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_GEOSL
AccessioniPrimary (citable) accession number: Q74CS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.